Unusual Features of the Light Response System Regulating Ferredoxin Gene Expression

  • W. F. Thompson
  • R. C. Elliott
  • L. F. Dickey
  • M. Gallo
  • T. J. Pedersen
  • D. A. Sowinski
Conference paper
Part of the NATO ASI Series book series (volume 50)


The ferrdoxin gene system. Recently we identified a cDNA clone encoding ferredoxin I and showed that the level of the corresponding transcript in pea seedlings is increased by white light (Sagar et al., 1988a, b) and by red light acting through the phytochrome system (Dobres et al., 1987; Kaufman et al., 1985, 1986). The light responses of this gene — which we have designated Fed-1 — differ strikingly from those of RbcS and Cab which have so far served as the main models for light responsive genes, providing us with an opportunity to significantly broaden our understanding of light regulatory mechanisms.


Light Response Transgenic Tobacco Seedling Nonhistone Chromosomal Protein Light Responsive Gene Specific mRNA Level 
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  1. Anderson LE (1986) Light/dark modulation of enzyme activity in plants. Advances in Botanical Research 12: 1–46.CrossRefGoogle Scholar
  2. Benfey PN, Chua N-H (1989) Regulated gene expression in transgenic plants. Science 244:174–181.PubMedCrossRefGoogle Scholar
  3. Bently DL, Groudine, M (1988) Sequence requirements for premature termination of transcription in the human c-myc gene. Cell 53:245–256.CrossRefGoogle Scholar
  4. Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12:5711–8721.CrossRefGoogle Scholar
  5. Buchanan BB (1980) Role of light in the regulation of chloroplast enzymes. Ann Rev Plant Physiol 31:371–374.CrossRefGoogle Scholar
  6. Bustos MM, Guiltinan MJ, Jordano J, Begum D, Kalkan FA, Hall, TC (1989) Regulation of ß-glucuronidase expression in transgenic tobacco plants by an A/T-rich, cis-acting sequence found upstream of a French bean ß-phaseolin gene. Plant Cell 1: 839–852.PubMedCrossRefGoogle Scholar
  7. Cammack R, Rao K, Hall DO (1985) Ferredoxins: Structure and function of a ubiquitous group of proteins. Physiol Veg 23:649–658.Google Scholar
  8. Chory J, Peto CA, Ashbaugh M, Saganich R, Pratt L, Ausubel F (1989) Different roles for phytochrome in etiolated and green plants deduced from characterization of Arabidopsh thaliana mutants. Plant Cell 1:867–880.PubMedCrossRefGoogle Scholar
  9. Crawford NA, Droux M, Kosower NS, Buchanan BB (1989) Evidence for function of the ferredoxin/thioredoxin system in the reductive activation of target enzymes of isolated intact chloroplasts. Arch Biochem Biophys 271:223–239.PubMedCrossRefGoogle Scholar
  10. Datta N, Cashmore AR (1989) Binding of a pea nuclear protein to promoters of certain photoregulated genes is modulated by phosphorylation. Plant Cell 1: 1069–1077.PubMedCrossRefGoogle Scholar
  11. Dobres MS, Elliott RC, Watson JC, Thompson WF (1987) A phytochrome-regulated pea transcript encodes ferredoxin I. Plant Mol Biol 8:53–59.CrossRefGoogle Scholar
  12. Dutton JE, Rogers LJ, Haslett BG, Takruri IAH, Gleaves JT, Boulter D (1980) Com parative studies of two ferredoxins from Pisum sativum L. J Exp Bot 31:379–391.CrossRefGoogle Scholar
  13. Elliott RC, Dickey LF, White MJ, Thompson WF (1989a) cis-Acting elements for light regulation of pea ferredoxin I gene expression are located within transcribed sequences. The Plant Cell 1:691–698.PubMedCrossRefGoogle Scholar
  14. Elliott RC, Pedersen TJ, Fristensky B, White MJ, Dickey LF, Thompson WF (1989b) Characterization of a single copy gene encoding ferredoxin I from pea. The Plant Cell 1:681–690.PubMedCrossRefGoogle Scholar
  15. Farnham PJ, Means, AL (1990) Sequences downstream of the transcription initiation site modulate the activity of the murine dihydrofolate reductase promoter. Mol Cell Biol 10:1390–1398.PubMedGoogle Scholar
  16. Fluhr R, Chua N-H (1986) Developmental regulation of two genes encoding ribulose bisphosphate carboxylase small subunit in pea and transgenic petunia plants: Phytochrome response and blue light induction. Proc Natl Acad Sci USA 83:2358–2362.PubMedCrossRefGoogle Scholar
  17. Fluhr R, Kuhlemeier c, Nagy f, Chua N-H (1986) Organ-specific and light-induced expression of plant genes. Science 232:1106–1112.PubMedCrossRefGoogle Scholar
  18. Goodwin G, Bustin M (1988) The HMG proteins and their genes. Architecture of Eukaryotic Genes. Weinheim, VCH Verlagsgesellschaft.Google Scholar
  19. Green PJ, Kay SA, Chua N-H (1987) Sequence-specific interactions of a pea nuclear factor with light-responsive elements upstream of the rbcS-3A gene. EMBO J 6:2543–2549.PubMedGoogle Scholar
  20. Guiltinan MJ, Thomas JC, Nessler CL, Thomas TL (1989) Expression of DNA binding proteins in carrot somatic embryos that specifically interact with a cis regulatory element of the French bean phaseolin gene. Plant Mol Biol 13:605–610.PubMedCrossRefGoogle Scholar
  21. Hoekema A, Hirsch PR, Hooykaas PJJ, Schilperoot RA (1983) A binary vector strategy based on separation of the vir-and T-region of the Agrvbacterium tumefasciens Ti-plasmid. Nature 303:179–180.CrossRefGoogle Scholar
  22. Holdsworth MJ, Laties GG (1989) Site-specific binding of a nuclear factor to the carrot extensin gene is influenced by both ethylene and wounding. Planta 179:17–23.CrossRefGoogle Scholar
  23. Horsch RB, Fry FE, Hoffman NL, Eicholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1231.CrossRefGoogle Scholar
  24. Hurt MM, Pandey NB, Marzluff WF (1989) A region in the coding sequence is required for high level expression of murine histone H3 gene. Proc Natl Acad Sci USA 86:4450–4454.PubMedCrossRefGoogle Scholar
  25. Jacobsen K, Laursen NB, Jensen EO, Marcker A, Poulsen C, Marcker KA (1990) HMG I-like proteins from leaf and nodule nuclei interact with different AT motifs in soybean nodulin promoters. Plant Cell 2:85–94.PubMedCrossRefGoogle Scholar
  26. Jensen EØ, Marker KA, Schell J, de Bruijn FJ (1988) Interaction of a nodule specific, trans-acting factor with distinct DNA elements in the soybean leghaemoglobin Ibc3 5′ upstream region. EMBO J 7:1265–1271.PubMedGoogle Scholar
  27. Jofuku KD, Okamuro JK, Goldberg RB (1987) Interaction of an embryo DNA binding protein with a soybean lectin gene upstream region. Nature 328:734–737.PubMedCrossRefGoogle Scholar
  28. Kaufman LS, Briggs WR, Thompson WF (1985) Phytochrome control of specific mRNA levels in developing pea buds: the presence of both very low fluence and low fluence responses. Plant Physiol 78:388–393.PubMedCrossRefGoogle Scholar
  29. Kaufman LS, Roberts LR, Briggs WR, Thompson WF (1986) Phytochrome control of specific mRNA levels in developing pea buds. Kinetics of accumulation, reciprocity, and escape kinetics of the low fluence response. Plant Physiol 81:1033–1038.PubMedCrossRefGoogle Scholar
  30. Kerppola TK, Kane CM (1988) Intrinsic sites of transcriptional termination and pausing in the c-myc gene. Mol Cell Biol 8:4389–4394.PubMedGoogle Scholar
  31. Kimat Y, Hase T (1989) Localization of ferredoxin isoproteins in mesophyll and bundle sheath cells in maize leaf. Plant Physiol 89:1193–1197.CrossRefGoogle Scholar
  32. Kuhlemeier CP, Green J, Chua N-H (1987) Regulation of gene expression in higher plants. Ann Rev Plant Physiol 38:221–257.CrossRefGoogle Scholar
  33. Marcotte WRJ, Russell SH, Quatrano RS (1989) Abscisic acid-responsive sequences from the Em gene of wheat. Plant Cell 1:969–976PubMedCrossRefGoogle Scholar
  34. Mösinger E, Batschauer A, Vierstra R, Apel K, Schäfer E (1987) Comparison of the effects of exogenous native phytochrome and in vivo irradiation on in vitro transcription in isolated nuclei from barley (Hordeum vulgare) Planta 170:505–514.CrossRefGoogle Scholar
  35. Rougvie AE, Lis JT (1988) The RNA polymerase II molecule at the 5′ end of the uninduced hsp70 gene of D. melanogaster is transcriptionally engaged. Cell 54:795–804.PubMedCrossRefGoogle Scholar
  36. Russell DW, Smith M, Cox D, Williamson VM, Young ET (1983) DNA sequences of two yeast promoter-up mutants. Nature 304:652–654.PubMedCrossRefGoogle Scholar
  37. Sagar AD, Briggs WR, Thompson WF (1988a) Nuclear-cytoplasmic partitioning of phytochrome-regulated transcripts in Pisum sativum. Plant Pbysiol 88:1397–1402.CrossRefGoogle Scholar
  38. Sagar AD, Horwitz BA, Elliott RC, Thompson WF, Briggs WR (1988b) Light effects on several chloroplast components in norfluorazon-treated pea seedlings. Plant Pbysiol 88:340–347.CrossRefGoogle Scholar
  39. Sakihama N, Shin M (1987) Evidence from high pressure liquid chromatography for the existence of two ferredoxins in plants. Arch Biochem Biophys 256:430–434.PubMedCrossRefGoogle Scholar
  40. Simpson J, Herrera-Estrella L (1990) Light-Regulated Gene Expression. Critical Reviews in Plant Sciences 9:95–109.CrossRefGoogle Scholar
  41. Smeekens S, Binsbergen JV, Weisbeek P (1985) The plant ferredoxin precursor: nucleotide sequence of a full length cDNA clone. Nucleic Acids Res 13:3179–3194.PubMedCrossRefGoogle Scholar
  42. Solomon MJ, Strauss F, Varshavsky A (1986) A mammalian high mobility group protein recognizes any stretch of six A·T base pairs in duplex DNA. Proc Natl Acad Sei (USA) 83:1276–1280.CrossRefGoogle Scholar
  43. Somers DE, Caspar T, Quail PH (1990) Isolation and characterization of a ferredoxin gene from Arabidopsis thaliana. Plant Physiol 93:572–577.PubMedCrossRefGoogle Scholar
  44. Spiker S (1984) High mobility group chromosomal proteins of wheat. J Biol Chem 259:12007–12013.PubMedGoogle Scholar
  45. Spiker S (1988a) Histone variants and high mobility group non-histone chromosomal proteins of higher plants: Their potential for forming a chromatin structure that is either poised for transcription or transcriptionally inert. Physiol Plant 75:200–213.CrossRefGoogle Scholar
  46. Spiker S (1988b) Histones and HMG proteins of higher plants. Architecture of Eukaryotic Genes. Weinhein, VCH Verlagsgesellschaft.Google Scholar
  47. Spiker S, Murray MG, Thompson WF (1983) DNase 1 sensitivity of transcriptionally active genes in intact nuclei and isolated chromatin of plants. Proc Natl Acad Sci (USA) 80:815–819.CrossRefGoogle Scholar
  48. Strahl K (1985) Naturally occurring poly(dA-dT) sequences are upstream promoter elements for constituitive transcription on yeast. Proc Natl Acad Sci (USA) 82:8419–8423.CrossRefGoogle Scholar
  49. Takahashi Y, Hase T, Wada K, Matsubara H (1983) Ferredoxins in developing spinach cotyledons: the presence of two molecular species. Plant Cell Physiol 24:189–198.Google Scholar
  50. Thompson WF (1988) Photoregulation: diverse gene responses in greening seedlings. Plant, Cell and Env 11:319–328.CrossRefGoogle Scholar
  51. Thompson WF, Kaufman LS, Watson JC (1985) Induction of plant gene expression by light. Bioessays 3:153–159.CrossRefGoogle Scholar
  52. Vorst O, v Dam F, Oosterhoff-Teertstra R, Smeekens S, Weisbeek P (1990) Tissue specific expression directed by an Arabidopsis thaliana pre-ferredoxin promoter in transgenic tobacco plants. Plant Mol Biol 14:491–499.PubMedCrossRefGoogle Scholar
  53. Wada K, Onda M, Matsubara H (1989) Amino acid sequences of ferredoxin isoproteins from radish roots. J Biochem 105:619–625.PubMedGoogle Scholar
  54. Weiss EA, Michael A, Yuan D (1989) Role of transcriptional termination in the regulation of μ mRNA expression in B lymphocytes. J Immunol 143:1046–1052.PubMedGoogle Scholar
  55. Wright S, Bishop JM (1989) DNA sequences that mediate attenuation of transcription from the mouse protooncogene myc. Proc Natl Acad Sci USA 86:505–509PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • W. F. Thompson
    • 1
  • R. C. Elliott
    • 1
    • 2
  • L. F. Dickey
    • 1
  • M. Gallo
    • 1
  • T. J. Pedersen
    • 1
  • D. A. Sowinski
    • 1
  1. 1.Departments of Botany and GeneticsNorth Carolina State UniversityRaleighUSA
  2. 2.John Innes InstituteNorwichUK

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