Phytochrome Regulation of Transcription: Biochemical and Genetic Approaches
Phytochrome action has been demonstrated to affect the transcription of a number of different genes in many different species (see reviews by Tobin and Silverthorne, 1985; Kuhlemeier et al., 1987). The effect may be either a positive or negative one. Other light receptors, circadian rhythms, and tissue type have also been shown to have effects on the transcription of phytochrome regulated genes. Particular short “light responsive elements” that can interact with protein factors have been identified upstream of a number of rbcS and cab genes encoding, respectively, the small subunit (SSU) of ribulose 1,5-bisphosphate carboxylase/oxygenase (Rubisco) and the major apoproteins of the photosystem II light-harvesting chlorophyll a/b-protein complex (LHCII). The evidence suggests these sequences play an important role in the overall response to light/dark conditions (reviewed in Silverthorne and Tobin, 1987; Benfey and Chua, 1989). There is also evidence that phytochrome action can alter RNA levels by effects on additional, post-transcriptional processes (Colbert, 1988; Thompson, 1988; Elliott et al., 1989), as well as influence many other processes, such as membrane permeability, that may not involve altered gene expression (Kendrick and Kronenberg, 1986). Although the phytochrome chromoprotein has itself been the subject of biochemical studies for many years, to date there is no clear understanding of the chain of events by which the phototransformation of phytochrome leads to specific transcriptional changes.
KeywordsSucrose Maize Chlorophyll Chrome Recombination
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- Green PJ, Yong M-H, Cuozzo M, Kano-Murakami Y, Silverstein P, Chua N-H (1988) Binding site rerequirements for pea nuclear protein factor GT-1 correlate with sequences required for light-dependent transcriptional activation of the rbcS-3A gene. EMBO J 6:2543–2549.Google Scholar
- Karlin-Neumann GA, Brusslan J, Tobin EM (1990) Manuscript in preparation.Google Scholar
- Kendrick RE, Kronenberg GHM (eds) (1986) Photomorphogenesis in Plants, Martinus Nijhoff, Boston.Google Scholar
- Koornneef M, Rolff E, Spruit CJP (1980) Genetic control of light-inhibited hypocotyl elongation in Ambidopsis thaliana (L.) Heynh. Z Pflanzenphysiol 100:147–160.Google Scholar
- Okubara PA, Tobin EM (1990) Manuscript in preparation.Google Scholar
- Tobin EM, Wimpee CF, Karlin-Neumann GA, Silverthorne J, Kohorn BD (1985) In: Arntzen CJ, Bogorad L, Bonitz S, Steinback KE (eds) The Molecular Biology of the Photosynthetic Apparatus Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, pp373–380.Google Scholar
- Tobin EM, Brusslan JA, Buzby JS, Karlin-Neumann, GA, Kehoe DM, Okubara PA, Rolfe SA, Sun L (1990) In: Herrmann R (ed) Plant Molecular Biology, NATO/ASI Series Plenum, New York, In press.Google Scholar