Excitation and adaptation of frog olfactory receptor neurones upon stimulation with second messengers and natural odorants

  • D. Schild
  • J. A. DeSimone
  • S. Hellwig
Conference paper
Part of the NATO ASI Series book series (volume 39)


Over the last five years it has become feasible to study the olfactory transduction process by the patch-clamp technique. Valuable contributions have come from Maue and Dionne (1987), Firestein and Werblin (1987,1989), Frings and Lindemann (1988), Zufall et al.(1989), Schild (1989), Schmiedel-Jacob et al.(1989), and Trotier (1986). From these studies we now know more about the ionic channels and the whole-cell voltage-gated currents present in these cells, though their physiological role has not been clarified in detail.


Olfactory Receptor Apical Side Basolateral Side Olfactory Receptor Neuron Olfactory Mucosa 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Firestein S, Werblin F (1987) Gated currents in isolated olfactory receptor neurons of the larval tiger salamander. Proc.Natl.Acad.Sci.USA 84:6292–6296PubMedCrossRefGoogle Scholar
  2. Firestein S, Werblin F (1989) Odor-induced membrane currents in vertebrate olfactory receptor neurons. Science 244:79–82PubMedCrossRefGoogle Scholar
  3. Frings S, Lindemann B (1988) Odorant response of isolated olfactory receptor cells is blocked by amilorid. J.Membrane Biol. 105:233–243CrossRefGoogle Scholar
  4. Hamill OP, Marty A, Neher E, Sakmann B, Sigworth FJ (1981) Improved patch-clamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflügers Arch. 391:85–100PubMedCrossRefGoogle Scholar
  5. Joshi et al.(1987) Spectrophotometric determination of cation concentrations in olfactory mucus. Neuroscience Letters 82:321–326PubMedCrossRefGoogle Scholar
  6. Kuba K, Nishi S (1976) Rhythmic hyperpolarizations and depolarizations of sympathetic ganglion cells induced by caffeine. J.Neurophysiol. 39:547–563PubMedGoogle Scholar
  7. Lancet D (1986) Vertebrate olfactory reception. In: Cowan WM (ed) Annual Reviews of Neuroscience, 9. Annual Reviews, Palo Alto 329–355Google Scholar
  8. Maue RA, Dionne VE (1987) Patch-clamp studies of isolated mouse olfactory receptor neurons. J.Gen.Physiol. 90:95–125PubMedCrossRefGoogle Scholar
  9. Persaud KC, Heck GL, DeSimone SK, Getchell TV, DeSimone JA (1988) Ion transport across the frog olfactory mucosa: the action of cyclic nucleotides on the basal and odorant-stimulated states. Biochim.Biophys.Acta 944:49–62PubMedCrossRefGoogle Scholar
  10. Restrepo D, Bruch RC (1989) Stimulus amino acids elicit rapid increase in intracellular calcium in dissociated olfactory neurons. Achems XI:150Google Scholar
  11. Schild D (1989) Whole-cell currents in olfactory receptor cells of Xenopus laevis. Exp.Brain Res. 78:223–232PubMedCrossRefGoogle Scholar
  12. Schmiedel-Jacob I, Anderson PAV, Ache BW (1989) Whole-cell recordings from lobster olfactory receptor cells: responses to current and odor stimulation. J.Neurophysiol. 61:994–1000Google Scholar
  13. Trotier D (1986) A patch-clamp analysis of membrane currents in salamander olfactory receptor cells. Pflügers Arch. 407:589–595PubMedCrossRefGoogle Scholar
  14. Zufall F, Stengl M, Hildebrand JG, Hatt H (1989) A patch clamp study of cultured olfactory receptor neurons from manduca sexta. Proceedings of the 17-th Göttingen Neurobiology Conference:75Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • D. Schild
    • 1
  • J. A. DeSimone
    • 1
  • S. Hellwig
    • 1
  1. 1.Physiology Dept.University of GöttingenGermany

Personalised recommendations