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DNA — Information and Aging: The Balance Between Alteration and Repair

  • Z. A. Medvedev

Abstract

The unique role of DNA in transfering genetic information from cell to cell and from generation to generation in evolution means that it is necessary to preserve the integrity of DNA molecules and their nucleotide sequences throughout millions of years and billions of replications. The complementarity of nucleotide base pairs in a double helix provides the main mechanism of the high accuracy of replication, but the potential of the hydrogen-bonded base pair complementarity alone would never be enough for this “thread of life” to preserve life on Earth in conditions where numerous environmental hazards, UV radiation, ionising radiation, free radicals, chemicals, and variations in temperature permanently produce changes in functional DNA molecules and errors during their replication. The highly efficient correction of DNA sequence errors is, therefore, absolutely essential and very many biochemical systems exist in cells and their nuclei for this purpose. The DNA repair system in prokaryotes and in germ cells of eukaryotes needs to preserve the integrity of genetic information over long evolutionary periods. However, somatic cells which function for only days, months or years and leave no progeny after the death of individual organisms do not need the same level of efficiency of correction of DNA sequence errors. Kirkwood and Holliday (1979) have suggested that a lower level of accuracy in synthesis and repair of macromolecules in somatic cells is (as compared with germ cells) an adaptation which saves energy; they postulate that aging occurs as a consequence (Kirkwood 1977; Kirkwood and Holliday 1979).

Keywords

Excision Repair Proliferate Tissue Peromyscus Leucopus Postreplication Repair Premature Aging Syndrome 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Adams RLP, Burdon RH (1985) The molecular biology of DNA methylation. Springer, New York BerlinCrossRefGoogle Scholar
  2. Adelman RC (1971) Age-dependent effects in enzyme induction - a biochemical expression of aging. Exp Gerontol 6: 75–87PubMedCrossRefGoogle Scholar
  3. Agarwal SS, Tuffner M, Loeb LA (1978) DNA replication in human lymphocytes during aging. J Cell Physiol 96: 235–243PubMedCrossRefGoogle Scholar
  4. Alexander P (1967) The role of DNA lesions in the process leading to ageing in mice. In: Woolhouse HW (ed) Aspects of the biology of ageing. Cambridge University Press, Cambridge, pp 29–50Google Scholar
  5. Barton RW, Yank WK (1975) Low molecular weight DNA polymerase: decreased activity in spleens of old mice. Mech Ageing Dev 4: 123–136PubMedCrossRefGoogle Scholar
  6. Bradley MO, Erickson LC, Kohn KW (1976) Normal DNA strand rejoining and absence of DNA cross-linking in progeroid and aging human cells. Mutat Res 37: 279–292PubMedCrossRefGoogle Scholar
  7. Brosius S, Grosse F, Krauss G (1983) Subspecies of DNA polymerase-a from calf thymus with different fidelity in copying synthetic template primers. Nucleic Acids Res II: 193–202Google Scholar
  8. Brown WT, Ford JP, Gershey EL (1980) Variations of DNA repair capacity in progeria cells unrelated to growth conditions. Biochem Biophys Res Commun 97: 347–353PubMedCrossRefGoogle Scholar
  9. Chen S, Srivastava BIS (1986) Fragmentation of DNA and chromatin during senescence in barley leaves. Mech Ageing Dev 34: 57–61PubMedCrossRefGoogle Scholar
  10. Chestanga CJ, Boyd V, Peterson L, Rusholow K (1975) Single-stranded regions in DNA of old mice. Nature 253: 130–131CrossRefGoogle Scholar
  11. Chestanga CJ, Tuttle M, Jacoboni A (1976) Changes in structural integrity of heart DNA from aging mice. Life Sci 18: 1405–1412CrossRefGoogle Scholar
  12. Chestanga CJ, Tuttle M, Jacoboni A, Johnson C (1977) Age-associated structural alterations in senescent mouse brain DNA. Biochim Biophys Acta 474: 180–187Google Scholar
  13. Cleaver JE (1967) Thymidine metabolism and cell kinetics. North Holland, AmsterdamGoogle Scholar
  14. Cleaver JE (1984) DNA repair deficiencies and cellular senescence are unrelated in Xerodermia pigmentosum cell lines. Mech Ageing Dev 27: 189–196PubMedCrossRefGoogle Scholar
  15. Collier JE, Popp DM, Lee WH, Regan JD (1982) DNA repair in a congeneic pair of mice with different longevities. Mech Ageing Dev 19: 141–156PubMedCrossRefGoogle Scholar
  16. Collins JM, Chu AK (1985) Reduction of DNA synthesis in aging but still proliferating cells. J Cell Physiol 124: 165–173PubMedCrossRefGoogle Scholar
  17. Cutler RG (1976) Cross-linkage hypothesis of aging; DNA adducts in chromatin as a primary aging process. In: Smith KC (ed) Proteins and other adducts to DNA. Their significance to ageing, carcinogenesis and radiation biology. Plenum, New York, pp 443–493Google Scholar
  18. Cutler RG (1979) Evolution of human longevity: a critical overview. Mech Ageing Dev 9: 337–354PubMedCrossRefGoogle Scholar
  19. Dean RG, Cutler RG (1978) Absence of significant age-dependent increase of single-stranded DNA extracted from mouse liver nuclei. Exp Gerontol 13: 287–292PubMedCrossRefGoogle Scholar
  20. Dell’Orco RT, Anderson LE (1981) Unscheduled DNA synthesis in human diploid cells of different donor ages. Cell Biol Intern Rep 5: 359–364CrossRefGoogle Scholar
  21. Drake JW (1969) Comparative rates of spontaneous mutations. Nature 221: 1132PubMedCrossRefGoogle Scholar
  22. Eichorn GL (1979) Aging, genetics and the environment.Potential of errors introduced into genetic information transfer by metal ions. Mech Ageing Dev 9: 291–301CrossRefGoogle Scholar
  23. Fairweather DS, Fox M, Margison GP (1987) The in vitro lifespan of MRC-5 cells is shortened by 5-azacytidine-induced demethylation. Exp Cell Res 168: 153–159PubMedCrossRefGoogle Scholar
  24. Falzone JA, Samids HV, Wulff VJ (1967) Cellular compensations and controls in the aging process. J Gerontol 22: 4 (part II) 42–51Google Scholar
  25. Fersht AR, Knill-Jones JW (1983) Fidelity of replication of bacteriophage 0X174 DNA in vitro and in vivo. J Mol Biol 165: 633–654PubMedCrossRefGoogle Scholar
  26. Finch CE (1979) Susceptibility of mouse liver DNA to digestion by SI nuclease: absence of age-related change. Age 2: 45–46CrossRefGoogle Scholar
  27. Francis AA, Lee WH, Regan JD (1981) The relationship of DNA excision repair of ultraviolet- induced lesions to the maximum lifespan of mammals. Mech Ageing Dev 16: 181–189PubMedCrossRefGoogle Scholar
  28. Fry M, Loeb LA, Martin GM (1981) On the activity and fidelity of chromatin-associated hepatic DNA polymerase-a in aging murine species of different life span. J Cell Physiol 106: 435–444PubMedCrossRefGoogle Scholar
  29. Fujiwara Y, Higashikawa T, Tatsumi M (1977) A retarded rate of DNA replication and normal level of DNA repair in Werner’s syndrome fibroblasts in culture. J Cell Physiol 92: 365–374PubMedCrossRefGoogle Scholar
  30. Gensler HL (1981) The effect of hamster age on UV-induced unscheduled DNA synthesis in freshly isolated lung and kidney cells. Exp Gerontol 16: 59–68PubMedCrossRefGoogle Scholar
  31. Gensler HL, Bernstein H (1981) DNA damage as the primary cause of aging. Q Rev Biol 56: 279–303PubMedCrossRefGoogle Scholar
  32. Glaser VM, Luchnik AN (1982) Decrease in the density of DNA topological turns during in vitro ageing of Syrian hamster cells. Exp Cell Res 139: 249–255PubMedCrossRefGoogle Scholar
  33. Goldstein BI, Rud SG, Polyakova LL (1966) Age changes of iron content in tissues and DNA of white rats. Vopr Med Khim 12: 618–621Google Scholar
  34. Grossman L (1981) Enzymes involved in the repair of damaged DNA. Arch Biochem Biophys 211: 511–522PubMedCrossRefGoogle Scholar
  35. Hall JD, Almy RE, Scherer KL (1982) DNA repair in cultured human fibroblasts does not decline with donor age. Exp Cell res 139: 351–359PubMedCrossRefGoogle Scholar
  36. Hall K, Hart RW, Benirschke AK, Walford RL (1984) Correlation between ultraviolet-induced DNA repair in primate lymphocytes and fibroblasts and species’ maximum achievable life span. Mech Ageing Dev 24: 163–173PubMedCrossRefGoogle Scholar
  37. Hanaoka F, Sayato J, Arai H, Hasegawa N, Inui N, Mitsui Y, Yamada MA (1983) Changes in DNA polymerase-α, ß, δ in mouse liver as a function of age. Mech Ageing Dev 23: 315–327PubMedCrossRefGoogle Scholar
  38. Hart RW, Setlow RB (1974) Correlation between deoxyribonucleic acid excision repair and lifespan in a number of mammalian species. Proc Natl Acad Sci USA 71: 2169–2173PubMedCrossRefGoogle Scholar
  39. Hart RW, Trosko JE (1976) DNA repair processes in mammals. Interdiscipl Topics Gerontol 9: 134–167 ( Karger, Basel )Google Scholar
  40. Hart RW, Turturro A (1981) Evolution and longevity-assurance processes. Naturwissenschaften 68: 552–557PubMedCrossRefGoogle Scholar
  41. Hart RW, D’Ambrosio SM, Ng KJ, Modak SP (1979a) Longevity, stability and DNA repair. Mech Ageing Dev 9: 203–223PubMedCrossRefGoogle Scholar
  42. Hart RW, Sacher GA, Hoskins TL (1979b) DNA repair in short- and long-lived rodent species. J Gerontol 34: 808–817PubMedGoogle Scholar
  43. Hasegawa N, Hanaoka F, Yamada M (1985) Does capacity of DNA replication change during in vitro ageing? Exp Cell Res 156: 478–486PubMedCrossRefGoogle Scholar
  44. Hennis HL III, Braid HL, Vincent RA Jr (1981) Unscheduled DNA synthesis in cells of different shape in fibroblast cultures from donors of various ages. Mech Ageing Dev 16: 355–361PubMedCrossRefGoogle Scholar
  45. Henson P (1978) The presence of single stranded regions in mammalian DNA. J Mol Biol 119: 487–506PubMedCrossRefGoogle Scholar
  46. Holliday R (1985) The significance of DNA methylation in cellular aging. In: Woodhead AO, Blackett AD, Hollaender A (eds) Molecular biology of aging. Plenum, New York, pp 269–283Google Scholar
  47. Holliday R, Pugh JE (1975) DNA modification mechanisms and gene activity during development. Science 187: 226–232PubMedCrossRefGoogle Scholar
  48. Hübscher U (1983) DNA polymerases in prokaryotes and eukaryotes: mode of action and biological implications. Experientia 39: 1–25PubMedCrossRefGoogle Scholar
  49. Ishikawa T, Tadayama S, Kitagawa T (1978) Autoradiographic demonstration of DNA repair synthesis in ganglion cells of aquarium fish at various age in vivo. Virchows Arch [Cell Pathol] 28: 235–242Google Scholar
  50. Johnson R, Chrisp C, Strehler BL (1972) Selective loss of ribosomal RNA genes during the aging of post mitotic tissues. Mech Ageing Dev 1: 183–198CrossRefGoogle Scholar
  51. Karron P, Ormerod MG (1973) Is the ability to repair damage to DNA related to the proliferative capacity of the cell? Biochim Biophys Acta 299: 54–64Google Scholar
  52. Kato H, Harada M, Tsuchiya K, Moriwaki K (1980) Absence of correlation between DNA repair in ultraviolet irradiated mammalian cells and the life span of the donor species. Jpn J Genet 55: 99–108CrossRefGoogle Scholar
  53. Kempf CH, Schmitt M, Danse JM, Kempf J (1984) Correlation of DNA repair synthesis with ageing in mice, evidenced by quantitative autoradiography. Mech Ageing Dev 26: 183–194PubMedCrossRefGoogle Scholar
  54. Kennah HE, Coetzee ML, Ove P (1985) A comparison of DNA repair synthesis in primary hepatocytes from young and old rats. Mech Ageing Dev 29: 283–298PubMedCrossRefGoogle Scholar
  55. Kirkwood TBL (1977) Evolution of ageing. Nature 270: 301–304PubMedCrossRefGoogle Scholar
  56. Kirkwood TBL (1981) Repair and its evolution: survival versus reproduction. In: Townsend CR, Calow P (eds) Physiological ecology: an evolutionary approach to resource use. Blackwell, Oxford, pp 165–194Google Scholar
  57. Kirkwood TBL, Holliday R (1979) The evolution of ageing and longevity. Proc R Soc Lond [Biol] 205: 531–546CrossRefGoogle Scholar
  58. Klass M, Nguyen PN, Dechavingny A (1983) Age-correlated changes in the DNA template in the nematode Caenorhabatis elegans. Mech Ageing Dev 22: 253–263PubMedCrossRefGoogle Scholar
  59. Kolata C (1985) Fitting methylation into development. Science 228: 1183–1184PubMedCrossRefGoogle Scholar
  60. Kondrashov AS (1988) Deleterious mutations and the evolution of reproduction. Nature, 336: 435–440PubMedCrossRefGoogle Scholar
  61. Lawson T, Stohs S (1985) Changes in endogenous DNA damage in aging mice in response to butylated hydroxyanisole and oltipraz. Mech Ageing Dev 30: 179–185PubMedCrossRefGoogle Scholar
  62. Lee MYWT, Tan CK, So AG, Downey KM (1980) Purification of deoxyribonucleic acid polymerase-ö from calf thymus: partical characterization of physical properties. Biochemistry 19: 2096–2101PubMedCrossRefGoogle Scholar
  63. Lindahl T (1976) New class of enzymes acting on damaged DNA. Nature 259: 64–66PubMedCrossRefGoogle Scholar
  64. Lindahl T (1987) Regulation and deficiencies in DNA repair. Br J Cancer 56: 91–95PubMedCrossRefGoogle Scholar
  65. Lindahl T, Nyberg B (1972) Rate of depurination of native deoxyribonucleic acid. Biochemistry 11: 3610–3618PubMedCrossRefGoogle Scholar
  66. Linn S, Kairis M, Holliday R (1976) Decreased fidelity of DNA polymerase activity isolated from human fibroblasts. Proc Natl Acad Sci USA 73: 2818–2822PubMedCrossRefGoogle Scholar
  67. Loeb LA, Kunkel TA (1982) Fidelity of DNA synthesis. Ann Rev Biochem 52: 429–457CrossRefGoogle Scholar
  68. Loeb LA, Reyland ME (1987) Fidelity of DNA synthesis. In: Eckstein F, Lilley DMJ (eds) Nucleic acids and molecular biology, vol. I. Springer, Berlin Heidelberg New York, pp 156–173Google Scholar
  69. Makinodan T, Peterson WJ, Baumgartner W, Kay MM (1977) Accumulation of DNA strand breaks with age in the liver and related organs. In: Platt D (ed) Liver and ageing. Schattauer, Stuttgart, pp 153–159Google Scholar
  70. Maslansky CJ, Williams GM (1985) Ultraviolet light-induced DNA repair synthesis in hepatocytes from species of different longevities. Mech Ageing Dev 29: 191–203PubMedCrossRefGoogle Scholar
  71. Massie HR, Baird MB, Nicolosi BJ, Samis HV (1972) Changes in the structure of rat liver DNA in relation to age. Arch Biochem Biophys 153: 733–741CrossRefGoogle Scholar
  72. Massie HR, Baird MB, McMahon MM (1975) Changes in the structure of the DNA of Drosophila melanogaster during development and aging. Mech Ageing Dev 4: 113–122PubMedCrossRefGoogle Scholar
  73. Medvedev ZA (1984) Age changes of chromatin. A review. Mech Ageing Dev 28: 139–154PubMedCrossRefGoogle Scholar
  74. Mitchinson JM (1971) The biology of the cell cycle. Cambridge University Press, CambridgeGoogle Scholar
  75. Modrich P (1987) DNA mismatch correction. Ann Rev Biochem 56: 435–466PubMedCrossRefGoogle Scholar
  76. Mori N, Goto S (1982) Estimation of the single-stranded region in the nuclear DNA of mouse tissues during aging with special reference to the brain. Arch Geront Geriatr I: 143–150Google Scholar
  77. Müller WEG, Zahn RK, Geurtsen W, Munsch N (1980) Age dependent aterations of DNA synthesis. Terminal deoxynucleotidyl transferase and DNA polymerase activities in bone marrow subpopulations from mice. Mech Ageing Dev 13: 119–126Google Scholar
  78. Murray V (1981) Properties of DNA polymerases from young and ageing human fibroblasts. Mech Ageing Dev 16: 327–343PubMedCrossRefGoogle Scholar
  79. Murray V, Holliday R (1981) Increased error frequency of DNA polymerases from senescent human fibroblasts. J Mol Biol 146: 55–76PubMedCrossRefGoogle Scholar
  80. Nakanishi K, Shima A, Fukuda M, Fujita S (1979) Age-associated increase of single-stranded regions in the DNA of mouse brain and liver cells. Mech Ageing Dev 10: 273–281PubMedCrossRefGoogle Scholar
  81. Niedermüller H, Hoffecker G, Skalicky M (1985) Changes of DNA repair mechanisms during the aging of the rat. Mech Ageing Dev 29: 221–238PubMedCrossRefGoogle Scholar
  82. Oesch F, Aulmann W, Platt KL, Doerjer G (1987) Individual differences in DNA repair capacities in man. Arch Toxicol [Suppl] 10: 172–179CrossRefGoogle Scholar
  83. Ono T, Okada S (1976) Comparative studies of DNA size in various tissues of mice during the aging process. Exp Gerantol II: 127–132Google Scholar
  84. Ono T, Tawa R, Shinya K (1986) Methylation of the C-myc gene changes during aging process of mice. Biochem Biophys Res Comm 139: 1299–1304PubMedCrossRefGoogle Scholar
  85. Ove P, Goetzee ML (1978) A difference in bleomycin-induced DNA synthesis between liver nuclei from mature and old rats. Mech Ageing Dev 8: 363–375PubMedCrossRefGoogle Scholar
  86. Paffenholz V (1978) Correlation between DNA repair of embryonic fibroblasts and different life span of 3 inbred mouse strains. Mech Ageing Dev 7: 131–150PubMedCrossRefGoogle Scholar
  87. Piantanelli L, Brogli R, Bevilacqua P, Fabris N (1978) Age-dependance of isoproterenol-induced DNA synthesis in sumandibular glands of BALB/c mice. Mech Ageing Dev 7: 162–169Google Scholar
  88. Plesko M, Richardson A (1984) Age-related changes in unscheduled DNA synthesis by rat hepatocytes. Biochem Biophys Res Comm 118: 730–735PubMedCrossRefGoogle Scholar
  89. Poison CDA, Webster GC (1982) Age-related DNA fragmentation in two varieties of Drosophila melanogaster, Phaseolus (coteledons) and three tissues of the mouse. Exp Gerontol 17: 11–17Google Scholar
  90. Price GB, Makinodan T (1973) Aging: alteration of DNA-protein information. Gerontologia 19: 58–70CrossRefGoogle Scholar
  91. Price GB, Modak SP, Makinodan T (1971) Age-associated changes in the DNA of mouse tissues. Science 171: 917–920PubMedCrossRefGoogle Scholar
  92. Razin A, Cedar H (1984) DNA methylation in eukayotic cells. Int Rev Cytol 92: 159–187PubMedCrossRefGoogle Scholar
  93. Regan JD, Cook JS, Lee WH (1968) Photoreactivation of amphibian cells in culture. J Cell Physiol 71: 173–176PubMedCrossRefGoogle Scholar
  94. Rothstein M (1982) DNA. In: Biochemical Approaches to Aging, pp 132–173. Academic Press. New YorkGoogle Scholar
  95. Robbins JH (1978) Significance of repair of human DNA: evidence from studies Xeroderma pigmentosum. J Natl Cancer Inst 61: 645–655PubMedGoogle Scholar
  96. Romanov GA, Vanyushin BF (1981) Methylation of reiterated sequences in mammalian DNAs. Effect of the tissue type, age, malignancy and hormonal induction. Biochim Biophys Acta 653: 204–218Google Scholar
  97. Samis HV, Falzone JA, Wulff VJ (1966) H3-Thymidine incorporation and mitotic activity in liver of rats various ages. Gerontologia 12: 79–88PubMedCrossRefGoogle Scholar
  98. Shearman CW, Loeb LA (1977) Depurination decreases fidelity of DNA synthesis in vitro. Nature 270: 337–338CrossRefGoogle Scholar
  99. Singhal RP, Mays-Hoopes LL, Eichhorn GL (1987) DNA methylation in aging of mice. Mech Ageing Dev 41: 199–210PubMedCrossRefGoogle Scholar
  100. Smith-Sonneborn J, Klass M (1974) Changes in the DNA synthesis pattern of Paramecium with increased clonal age and interfission time. J Cell Biol 61: 591–598PubMedCrossRefGoogle Scholar
  101. So AG, Downey KM (1988) Mammalian DNA polymerases a and d: current status in DNA replication. Biochemistry 27: 4991–4995CrossRefGoogle Scholar
  102. Strauss BS (1985) Cellular aspects of DNA repair. Adv Cancer Res 45: 45–101PubMedCrossRefGoogle Scholar
  103. Strehler BL, Chang MP, Johnson LK (1979) Loss of hybridizable ribosomal DNA from human post-mitotic tissues during aging: I. Age-dependent loss in human myocardium. Mech Ageing Dev 11: 371–378Google Scholar
  104. Struchkov VA (1962) On nature of superpolymer DNA. Biophysica (Moscow) 7: 538–550Google Scholar
  105. Su CHM, Brash DE, Turturo A, Hart RW (1984) Longevity-dependent organ-specific accumu-lation of DNA damage in two closely related murine species. Mech Ageing Dev 27: 239–247PubMedCrossRefGoogle Scholar
  106. Swenberg SA, Fennell TR (1987) DNA damage and repair in mouse liver. Arch Toxicol [Suppl] 10: 162–171CrossRefGoogle Scholar
  107. Targovnik HS, Locher SE, Hart TF, Hariharan PV (1984) Age-related changes in the excision repair capacity of Turbatrix aceti. Mech Ageing Dev 27: 73–81PubMedCrossRefGoogle Scholar
  108. Tice RR (1978) Aging and DNA repair capability. In: Schneider EL (ed) The genetics of aging. Plenum, New York, pp 53–89Google Scholar
  109. Treton JA, Courtois Y (1982) Correlation between DNA excision repair and mammalian lifespan in lens epithelial cells. Cell Biol Int Rep 6: 253–260PubMedCrossRefGoogle Scholar
  110. Turner DR, Morley AA, Seshadri RS, Sorrell JR (1981) Age-related variations in human lymphocyte DNA. Mech Ageing Dev 17: 305–309PubMedCrossRefGoogle Scholar
  111. Turner DR, Griffith VC, Morley AA (1982) Ageing in vivo does not alter the kinetics of DNA strand break repair. Mech Ageing Dev 19: 325–331PubMedCrossRefGoogle Scholar
  112. Vanyushin BF, Nemirovsky LE, Belozersky AN (1973) The 5-methylcytosine in DNA of rats: tissue and age specificity. Gerontologia 19: 138–152PubMedCrossRefGoogle Scholar
  113. von Hahn HP (1970) Structural and functional changes in nucleoprotein during ageing of the cell. Gerontologia 16: 116–128CrossRefGoogle Scholar
  114. Wegener K, Hollweg S, Maurer W (1964) Autoradiographische Bestimmung der DNA-Verdopp- lungszeit und anderer Teil-phasen des Zell-zyklus bei fetalen Zellarten der Ratte. Z Zellforsch Mikrosk Anat 63: 309–326PubMedCrossRefGoogle Scholar
  115. Wheeler KT, Lett JT (1972) Formation and rejoining of DNA strand breaks in irradiated neurons in vivo. Radiat Res 52: 59–67PubMedCrossRefGoogle Scholar
  116. Wheeler KT, Lett JT (1974) On the possibility that DNA repair is related to age in non-dividing cells. Proc Natl Acad Sci USA 71: 1862–1865PubMedCrossRefGoogle Scholar
  117. Williams JR, Dearfield KL (1981) DNA damage and repair in aging mammals. In: Florini JR (ed) CRC handbook of biochemistry in aging. CRC, Boca Raton, Florida, pp 25–48Google Scholar
  118. Wilson VL, Jones PA (1983) DNA methylation decreases in ageing but not in immortal cells. Science 220: 1055–1057PubMedCrossRefGoogle Scholar
  119. Woodhead AD, Setlow RB, Grist E (1980) DNA repair and longevity in three species of cold blooded vertebrates. Exp Gerontol 15: 301–304PubMedCrossRefGoogle Scholar
  120. Wright JR, Stephens RE, Ford KC, Thibert P, Yates AJ (1986) DNA excision repair in spontaneously diabetic BB Wistar rats. Mech Ageing Dev 36: 109–116PubMedCrossRefGoogle Scholar
  121. Zahn RK (1983) Measurement of the molecular weight distributions in human muscular deoxyribonucleic acid. Mech Ageing Dev 22: 355–379PubMedCrossRefGoogle Scholar
  122. Zahn RK, Reinmtiller J, Beyer R, Poneljak V (1987) Age-correlated DNA damage in human muscle tissue. Mech Ageing Dev 41: 73–114PubMedCrossRefGoogle Scholar
  123. Zweidler A (1984) Core histone variants of the mouse; primary structure and differential ex-pression. In: Stein GS (ed) Histone genes and histone gene expression. Wiley, New York, pp 373–395Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Z. A. Medvedev
    • 1
  1. 1.Genetics DivisionNational Institute for Medical ResearchMill Hill, LondonUK

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