Investigation of Liver Pathology with Magnetite-Dextran Superparamagnetic Nanoparticles as New MRI Contrast Agent
In the last ten years, many authors have described the preparation of magnetic iron-dextran particles by precipitating an aqueous solution containing ferric chloride, ferrous chloride, and dextran by adding an alkaline base while stirring and heating the solution. Magnetite-Dextran (MD) nanoparticles were obtained by modifying the following aspects of the Molday procedure (Molday and Mackenzie 1982): dextran concentration, Fe3+/FE2+ molar ratio, titration, and heating parameters. The size distribution of the nanoparticles was analyzed with an N4M nanosizer (Coultronics, France) and the distribution of iron core dimensions was examined by transmission electron microscopy (TEM, JEOL 100 B). Dextran and iron concentrations were measured by spectrophotometric methods (phenol/sulphuric acid (Dubois et al. 1956) and HC1 30% (Pouliquen 1988), at 490 nm and 340 nm respectively. Rx and R2 relaxivities were determined on a PC 20 spectrometer (Bruker, France) at 0.47 T, 20 MHz, 4° C, and 37° C. T1 was measured by a nonlinear least squares fit calculation to 8 data points, generated with an inversion-recovery pulse sequence. T2 was obtained from 10 data points, generated with a Meiboom-Gill-Carr-Purcell sequence.
KeywordsNormal Liver Tissue Iron Oxide Core Dextran Concentration Iron Iron High Magnetic Field Gradient
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