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Biosynthesis and Genetics of C3

  • Conference paper
The Third Component of Complement

Part of the book series: Current Topics in Microbiology and Immunology ((CT MICROBIOLOGY,volume 153))

Abstract

The biosynthesis, regulation of synthesis, and genetics of C3 have been extensively studied (for reviews see COLTEN 1976; COLE and COLTEN 1988; FEY et al. 1983). In fact, perhaps more is known about these aspects of C3 biology than about any other complement component. This extensive characterization is due primarily to the central role of C3 in complement activation and regulation as well as in the host immune response. Certain practical aspects have also contributed to the diverse background on C3, including its early characterization relative to many other complement proteins and its ready availability due to its high serum concentration.

This research was supported by NIH grants AI 19222 (to B.F.T.) and AI 19551 (to G.H.F.).

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References

  • Alper CA (1973) Genetics and the C3 molecule. Vox Sang 25: 1–8

    PubMed  CAS  Google Scholar 

  • Alper CA, Propp RP (1968) Genetic polymorphism of the third component of human complement (C3). J Clin Invest 47: 2181–2191

    PubMed  CAS  Google Scholar 

  • Alper CA, Rosen FS (1976) Genetics of the human complement system. Adv Hum Genet 7: 141–188

    PubMed  CAS  Google Scholar 

  • Alper CA, Rosen FS (1981) Complement deficiencies in humans. In: Clinical immunology update. Elsevier, New York, pp 59–75

    Google Scholar 

  • Alper CA, Rosen FS (1984) Inherited deficiencies of complement proteins in man. Springer Semin Immunopath 7: 251–261

    CAS  Google Scholar 

  • Alper CA, Johnson AM, Birtch AM, Moore FD (1969) Human C3: evidence for the liver as the primary site of synthesis. Science 163: 286–288

    PubMed  CAS  Google Scholar 

  • Alper CA, Colten HR, Rosen FS, Rabsonn AR, Macnab GM, Gear JSS (1972) Homozygous deficiency of C3 in a patient with repeated infections. Lancet II: 1179–1181

    Google Scholar 

  • Ballow M, Shira JE, Harden L, Yang SY, Day NK (1975) Complete absence of the third component of complement in man. J Clin Invest 56: 703–710

    PubMed  CAS  Google Scholar 

  • Barnum SR, Volanakis JE (1985) Biosynthesis of complement protein D by HepG2 cells: a comparison of D produced by HepG2 cells, U937 cells and blood monocytes. Eur J Immunol 15: 1148–1151

    PubMed  CAS  Google Scholar 

  • Barnum SR, Amiguet P, Amiguet-Barras F, Fey G, Tack BF (1989) Complete intron/exon organization of DNA encoding the α chain of human C3. J Biol Chem 264: 8471–8474

    PubMed  CAS  Google Scholar 

  • Becherer JD, Lambris JD (1988) Identification of the C3b-receptor binding domain in third component of complement. J Biol Chem 263: 14586–14591

    PubMed  CAS  Google Scholar 

  • Bednarczyk JL, Capra JD (1988) Posttranslational processing of the murine third component of complement. Scand J Immunol 27: 83–95

    PubMed  CAS  Google Scholar 

  • Behrendt N (1985) Human complement component C3: characterization of active C3 S and C3 F, the two common genetic variants. Mol Immunol 22: 1005–1008

    PubMed  CAS  Google Scholar 

  • Belt KT, Carroll MC, Porter RR (1984) The structural basis of the multiple forms of human complement C4. Cell 36: 907–914

    PubMed  CAS  Google Scholar 

  • Breathnach R, Benoist C, O’Hare K, Gannon F, Chambon P (1978) Ovalbumin gene: evidence for a leader sequence in rnRNA and DNA sequences at the exon-intron boundaries. Proc Natl Sci USA 75: 4853–4857

    CAS  Google Scholar 

  • Carroll MC, Capra JD (1979) Molecular weight variations in the polypeptide subunits of the third and fourth components of complement derived from various mammalian species. Fed Proc 38: 1291

    Google Scholar 

  • Carroll MC, Campbell RD, Bentley Dr, Porter RR (1984) A molecular map of the human major histocompatability complex class III region linking complement genes C4, C2 and factor B. Nature 307: 237–241

    PubMed  CAS  Google Scholar 

  • Churchill WH, Weintraub RM, Borsos T, Rapp HJ (1967) Mouse complement: the effect of sex hormones and castration on two of the late acting components. J Exp Med 125:657–673

    PubMed  CAS  Google Scholar 

  • Cole FS, Colten HR (1988) Complement biosynthesis. In: Till GO, Rother KO (eds) The complement system. Springer, Berlin Heidelberg New York, pp 44–70

    Google Scholar 

  • Colten HR (1972) Ontogeny of the human complement system: in vitro biosynthesis of individual complement components by fetal tissues. J Clin Invest 51: 725–750

    PubMed  CAS  Google Scholar 

  • Colten HR (1976) Biosynthesis of complement. Adv Immunol 22: 67–118

    PubMed  CAS  Google Scholar 

  • Colten HR (1985) Biology of disease. Molecular basis of complement deficiency syndromes. Lab Invest 52: 468–474

    PubMed  CAS  Google Scholar 

  • Daoudaki ME, Becherer JD, Lambris JD (1988) A 34-amino acid peptide of the third component of complement mediates properdin binding. J Immunol 140: 1577–1580

    PubMed  CAS  Google Scholar 

  • Darlington GJ, Wilson Dr, Lachmann LB (1986) Monocyte-conditioned medium, inter-leukin 1, and tumor necrosis factor stimulate the acute phase response in human hepatoma cells in vitro. J Cell Biol 103: 787–793

    PubMed  CAS  Google Scholar 

  • Davies KE, Jackson J, Williamson R, Harper PS, Sarfarazi M, Meredith L, Fey GH (1983) Linkage analysis of myotonic dystrophy and sequences on chromosome 19 using a cloned complement 3 gene probe. J Med Genet 20: 259–263

    PubMed  CAS  Google Scholar 

  • de Bruijn MHL, Fey GH (1985) Human complement component C3: cDNA coding sequence and derived primary structure. Proc Natl Acad Sci USA 82: 708–712

    PubMed  Google Scholar 

  • Dodgson JB, Engel JD (1983) The nucleotide sequence of the adult chicken α-globin genes. J Biol Chem 258: 4623–4629

    PubMed  CAS  Google Scholar 

  • Domdey H, Wierbauer K, Kazmaier M, Müller V, Odink K, Fey G (1982) Characterization of the mRNA and cloned cDNA specifying the third component of mouse complement. Proc Natl Acad Sci USA 79: 7619–7623

    PubMed  CAS  Google Scholar 

  • Eggertsen G, Lundwall A, Hellman U, Sjoquist J (1983) Antigenic relationships between uman and cobra complement factors C3 and cobra venom factor (CVF) from the Indian cobra (Naja naja). J Immunol 131: 1920–1923

    PubMed  CAS  Google Scholar 

  • Einstein LP. Hansen P, Ballow M, Davis E, Davis JS, Alper CA, Rosen FS, Gölten HR (1977) Biosynthesis of the third component of complement (C3) in vitro by monocyte from both normal and homozygous C3-deficient humans. J Clin Invest 60: 963–969

    PubMed  CAS  Google Scholar 

  • Felber BK, Orkin SH, Hamer DH (1982) Abnormal RNA splicing causes one form of a thalassemia. Cell 29: 895–902

    PubMed  CAS  Google Scholar 

  • Fey G, Gauldie J (1989) The acute phase response of the liver in inflammation. In: Popper H, Schaffner F (eds) Progress in liver disease. Saunders, Philadelphia (to be published)

    Google Scholar 

  • Fey G, Odink K, Chapuis RM (1980) Synthesis of the mouse complement component C4 (Ss-protein) by peritoneal macrophages: kinetics of secretion and glycosylation of the subunits. Eur J Immunol 10: 75–82

    PubMed  CAS  Google Scholar 

  • Fey G, Domdey H, Wiebauer K, Whitehead AS, Odink K (1983) Structure and expression of the C3 gene. Springer Semin Immunopathol 6: 119–147

    Google Scholar 

  • Fey G, Wiebauer K, Domdey H, Kazmaier M, Southgate C, Müller V (1984) Structural analysis of cloned mouse and human DNA sequences specifying C3, the third component of complement. In: Muftuoglu AU, Barlas N (eds) Recent advances in immunology. Plenum, New York, pp 101–106

    Google Scholar 

  • Goldberger G, Thomas ML, Tack BF, Williams J, Colten HR, Abraham GN (1981) NH2-terminal structure and cleavage of guinea pig pro-C3, the precursor of the third complement component. J Biol Chem 256: 12617–12619

    PubMed  CAS  Google Scholar 

  • Goldberger G, Arnaout MA, Aden D, Kay R, Rits M, Colten HR (1984) Biosynthesis and postsynthetic processing of human C3b/C4b inactivator (factor I) in three hepatoma cell lines. J Biol Chem 259: 6492–6497

    PubMed  CAS  Google Scholar 

  • Green MR (1986) Pre-mRNA splicing. Annu Rev Genet 20: 671–708

    PubMed  CAS  Google Scholar 

  • Guiguet M, Exilie Frigere M-F, Dethieux M-C, Bidan Y, Mack G (1987) Biosynthesis of the third component of complement in rat liver epithelial cell lines and its stimulation by effector molecules from cultured human mononuclear cells. In Vitro Cell Dev Biol 23: 821–829

    PubMed  CAS  Google Scholar 

  • Hamilton AO, Jones L, Morrison L, Whaley K (1987) Modulation of monocyte complement synthesis by interferons. Biochem J 242: 809–815

    PubMed  CAS  Google Scholar 

  • Hase S, Kikuchi N, Ikenaka T, Inoue K (1985) Structure of sugar chains of the third component of human complement. J Biochem 98: 963–874

    Google Scholar 

  • Hirani S, Lambris JD, Müller-Eberhard HJ (1986) Structural analysis of the asparagine-linked oligosaccharides of human complement component C3. Biochem J 233: 613–616

    PubMed  CAS  Google Scholar 

  • Hostetter MK, Krueger RA, Schmeling DJ (1984) The biochemistry of opsonization: central role of the reactive thiol ester of the third component of complement. J Infect Dis 150:653–661

    PubMed  CAS  Google Scholar 

  • Hubbard SC, Ivatt RJ (1981) Synthesis and processing of asparagine-linked oligosaccharides. Annu Rev Biochem 50: 555–583

    PubMed  CAS  Google Scholar 

  • Iijima M, Tobe T, Sakamoto T, Tomita M (1984) Biosynthesis of the internal thiolester bond of the third component of complement. J Biochem 96: 1539–1546

    PubMed  CAS  Google Scholar 

  • Johnson E, Hetland G (1988) Mononuclear phagocytes have the potential to synthesize the complete functional complement system. Scand J Immunol 27: 489–493

    PubMed  CAS  Google Scholar 

  • Kai C, Yoshikawa Y, Yamamuchi K, Okada H (1983) Isolation and identification of the third component of complement of Japanese quails. J Immunol 130: 2814–2820

    PubMed  CAS  Google Scholar 

  • Kan CC, Solomon E, Belt KT, Chain AC, Hiorns LR, Fey G (1985) Nucleotide sequence of cDNA encoding human a2-macroglobulin and assignment of the chromosomal locus. Proc Natl Acad Sci USA 82: 2282–2286

    PubMed  CAS  Google Scholar 

  • King CR, Piatigorsky J (1983) Alternative RNA splicing of the murine a A-crystallin gene: protein-coding information within an intron. Cell 32: 707–712

    PubMed  CAS  Google Scholar 

  • Knowles BB, Howe CC, Aden DP (1980) Human hepatocellular carcinoma cell lines secrete the major plasma proteins and hepatitis B surface antigen. Science 209: 497–499

    PubMed  CAS  Google Scholar 

  • Kohler PF, Müller-Eberhard HJ (1967) Immunochemical quantitation of the third, fourth and fifth components of human complement: concentrations in the serum of healthy adults. J Immunol 99: 1211–1216

    PubMed  CAS  Google Scholar 

  • Koj A (1985) Definition and classification of acute phase proteins. In: Gordon AH, Koj A (eds) The acute phase response to injury and infection. Elsevier, Amsterdam, pp 139–144

    Google Scholar 

  • Koj A (1986) Biological functions of acute-phase proteins and the cytokines involved in their induced synthesis. In: Reutter W, Popper H, Arias IM, Heinrich PC, Keppler D, Landmann L (eds) Modulation of liver cell expression. MTP Press, Lancaster, pp 331–341

    Google Scholar 

  • Kusano M, Choi N, Tomita M, Yamamoto K, Migita S, Sekiya T, Nishimura S (1986) Nucleotide sequence of cDNA and derived amino acid sequence of rabbit complement component C3 α chain. Immunol Invest 15: 365–378

    PubMed  CAS  Google Scholar 

  • Kushner I (1982) The phenomenon of the acute phase response. Ann NY Acad Sci 389:39–48

    PubMed  CAS  Google Scholar 

  • Lambris JD (1988) The multifunctional role of C3, the third component of complement. Immunol Today 9: 387–393

    PubMed  CAS  Google Scholar 

  • Lambrid JD, Müller-Eberhard HJ (1986) The multifunctional role of C3: structural analysis of its interactions with physiological ligands. Mol Immunol 23: 1237–1242

    Google Scholar 

  • Lambris JD, Ganu VS, Hirani S, Müller-Eberhardt HJ (1985) Mapping of the C3d receptor (CR2)-binding site and a neoantigenic site in the C3d of the third component of complement. Proc Natl Acad Sci USA 82: 4235–239

    PubMed  CAS  Google Scholar 

  • Lambris JD, Avila D, Becherer JD, Müller-Eberhard HJ (1988) A discontinuous factor H binding site in the third component of complement as delineated by synthetic peptides. J Biol Chem 263: 2147–2150

    Google Scholar 

  • Lappin D, Hamilton AD, Morrison L, Aref M, Whaley K (1986) Synthesis of complement components (C3, C2, B and Cl-inhibitor) and lysozyme by human monocytes and macrophages. J Clin Lab Immunol 20: 101–105

    PubMed  CAS  Google Scholar 

  • Lernhardt W, Rascke Wc, Melchers F (1986) Alpha-type B cell growth factor and complement component C3: their possible structural relationship. In: Melchers F, Potter M (eds) Mechanisms in B-cell neoplasia. Springer, Berlin Heidelberg New York, pp 98–104 (Current topics in microbiology and immunology, vol 132)

    Google Scholar 

  • Levi-Strauss M, Mallat M (1987) Primary cultures of murine astrocytes produce C3 and factor B, two components of the alternative pathway of complement activation. J Immunol 139: 2361–2366

    PubMed  CAS  Google Scholar 

  • Lundwall A, Wetsel RA, Domdey H, Tack BF, Fey GH (1984) Structure of murine complement component C3. 1. Nucleotide sequence of cloned complementary and genomic DNA coding for the β chain. J Biol Chem 259: 13851–13856

    PubMed  CAS  Google Scholar 

  • Lundwall AB, Wetsel RA, Kristensen TK, Whitehead AS, Woods E, Ogden RC, Colten HR, Tack BF (1985) Isolation and sequence of a cDNA clone encoding the fifth complement component. J Biol Chem 260: 2108–2112

    PubMed  CAS  Google Scholar 

  • Lusis AJ, Heinzmann C, Sparkes RS, Scott J, Knott TJ, Geller R, Sparkes MC, Mohandas T (1986) Regional mapping of human chromosome 19: organization of the genes for plasma lipid transport (APOC1,-C2, and-E and LDLR) and the genes C3, PEPD, and GPL Proc Natl Acad Sci USA 83: 3929–3933

    PubMed  CAS  Google Scholar 

  • Maniatis T, Goodbourn S, Fischer JA (1987) Regulation of inducible and tissue-specific gene expression. Science 236: 1237–1245

    PubMed  CAS  Google Scholar 

  • Minta JO, Isenman DE (1987) Biosynthesis of the third component of complement by the human monocyte-like cell line, U937. Mol Immunol 24: 1105–1111

    PubMed  CAS  Google Scholar 

  • Morris KM, Aden DP, Knowles BB, Colten HR (1982b) Complement biosynthesis by the human hepatoma-derived cell line HepG2. J Clin Invest 70: 906–913

    PubMed  CAS  Google Scholar 

  • Morris KM, Goldberger G, Colten HR, Aden DP, Knowles BB (1982a) Biosynthesis and processing of a human precursor complement, pro-C3, in a hepatoma-derived cell line. Science 215: 399–400

    PubMed  CAS  Google Scholar 

  • Mount SM (1982) A catalogue of splice junction sequences Nucleic Acids Res 10: 459–472

    PubMed  CAS  Google Scholar 

  • Müller-Eberhard HJ, Schreiber RD (1980) Molecular biology and chemistry of the alternative pathway of complement. Adv Immunol 29: 1–53

    PubMed  Google Scholar 

  • Natsumme-Sakai S, Moriwaki K, Amano S, Hayakawa JI, Kaidoh T, Takahashi M (1979) Genetic mapping of the locus controlling structural variations of murine C3 in the chromosome 17. J Immunol 123: 216–221

    Google Scholar 

  • Nichols WK (1984) LPS stimulation of complement (C3) synthesis by a human monocyte cell line. Complement 1: 108–115

    PubMed  CAS  Google Scholar 

  • Nonaka M, Iwaki M, Nakai C, Nozaki M, Kaidoh T, Nonaka M, Natsuume-Sakai S, Takahashi M (1984a) Purification of a major serum protein of rainbow trout (Salmo gairdner.) homologous to the third component of mammalian complement. J Biol Chem 259:6327–6333

    PubMed  CAS  Google Scholar 

  • Nonaka M, Fujii T, Kaidoh T, Natsumme-Sakai S, Nonaka M, Yamaguchi N, Takahashi M (1984b) Purification of a lamprey complement protein homologous to the third component of the mammalian complement system. J Immunol 133: 3242–3249

    PubMed  CAS  Google Scholar 

  • Ogata RT, Schreffler DC, Sepich DS, Lilly SP (1983) cDNA clone spanning the oc-y subunit in the precursor of the fourth complement component. Proc Natl Acad Sci USA 80:5061–5065

    PubMed  CAS  Google Scholar 

  • Padgett RA, Grabowski PJ, Konarska MM, Sciler S, Sharp PA (1986) Splicing of messenger RNA precursors. Annu Rev Biochem 55: 119–1150

    Google Scholar 

  • Penalva DaSilva F, Hoecker GF, Day NK, Vienne K, Rubinstein P (1978) Murine complement component 3: genetic variation and linkage to H-2. Proc Natl Acad Sci USA 75: 963–965

    Google Scholar 

  • Propp RP, Alper CA (1968) C’3 synthesis in the human fetus and the lack of transplacental passage. Science 162: 612–614

    Google Scholar 

  • Pussell BA, Bourke E, Nayef M, Morris S, Peters K (1980) Complement deficiency and nephritis: a report of a family. Lancet 1: 675–677

    PubMed  CAS  Google Scholar 

  • Rittner C, Schneider PM (1988) Genetics and polymorphism of the complement components. In: Rother K, Till GO (eds) The complement system. Springer, Berlin Heidelberg New York, pp 80–135

    Google Scholar 

  • Ross SC, Denson P (1984) Complement deficiency states and infection: epidemiology, pathogene-sis and consequences of neisserial and other infections in an immune deficiency. Medicine 63: 243–273

    PubMed  CAS  Google Scholar 

  • Ruddy S, Carpenter CB, Chin KW, Knostman JN, Soter NA, Götze O, Müller-Eberhard HJ, Austen KF (1975) Human complement metabolism: an analysis of 144 studies. Medicine 54: 165–178

    Google Scholar 

  • St John Sutton M, Strunk RC, Cole FS (1986) Regulation of the synthesis of the third component of complement and factor B in cord blood monocytes by lipopolysaccharide. J Immunol 136: 1366–1372

    Google Scholar 

  • Sekizawa A, Fujii T, Katagiri C (1984) Isolation and characterization of the third component of complement of the serum of the clawed frog, Xenopus laevis. J Immunol 133:1436–1443

    PubMed  CAS  Google Scholar 

  • Shaw DJ, Meredith AL, Brook JD, Sarfarazi M, Harley HG, Huson SM, Bell GI, Harper PS (1986) Linkage relationships of the insulin receptor gene with the complement component 3, LDL receptor, apolipoprotein C2 and myotonic dystrophy loci on chromosome 19. Hum Genet 74: 267–269

    PubMed  CAS  Google Scholar 

  • Sottrup-Jensen L (1987) Alpha2-macroglobulin and related thiol ester plasma proteins. In: Putman FW (ed) The plasma proteins: structure, function, and genetic control, vol 5. Academic, Orlando, pp 192–291

    Google Scholar 

  • Steiner DF, Docherty K, Carroll R (1984) Golgi/Granule processing of peptide hormone and neuropeptide precursors: a mini review. J Cell Biochem 24: 121–130

    PubMed  CAS  Google Scholar 

  • Strunk RC, Tashjian AH, Colten HR (1975) Complement biosynthesis in vitro by rat hepatoma cell strains. J Immunol 114: 331–335

    PubMed  CAS  Google Scholar 

  • Strunk RC, Kunke KS, Giclas PC (1983) Human peripheral blood monocyte-derived macrophages produced haemolytically active C3 in vitro. Immunology 49: 483–488

    Google Scholar 

  • Strunk RC, Whitehead AS, Cole FS (1985) Pretranslational regulation of the synthesis of the third component of complement in human mononuclear phagocytes by the lipid A portion of lipopolysaccaride. J Clin Invest 76: 985–990

    PubMed  CAS  Google Scholar 

  • Strunk RC, Eilden DM, Mason RJ (1988) Pulmonary aveolar type II epithelial cells synthesize and secrete proteins of the classical and alternative complement pathways. J Clin Invest 81: 1419–1426

    PubMed  CAS  Google Scholar 

  • Sundstrom C, Nilsson K (1976) Establishment and characterization of a human histiocytic lymphoma cell line (U937). Int J Cancer 17: 565–577

    PubMed  CAS  Google Scholar 

  • Szpirer J, Islam MQ, Cooke NE, Szpirer C, Evan G (1988) Assignment of three rat genes coding for plasma proteins, transferrin, third component of complement, and beta-fibrinogen to rat chromosomes 8, 9, and 2. Cytogenet Cell Genet 47: 42–45

    PubMed  CAS  Google Scholar 

  • Tack BF (1983) The β-Cys-γ-Glu thiolester bond in human C3, C4 and α2macroglobulin. Springer Semin Immunopathol 6: 259–282

    Google Scholar 

  • Tack BF, Prahl JW (1976) Third component of human complement: purification from plasma and physicochemical characterization. Biochemistry 15: 4513–4521

    PubMed  CAS  Google Scholar 

  • Tartakoff AM (1983) Perturbation of vesicular traffic with the carboxylic iohophore monensin. Cell 32: 1026–1028

    PubMed  CAS  Google Scholar 

  • Thomas ML, Tack BF (1983) Identication and alignment of a thiol ester site in the third component of guinea pig complement. Biochemistry 22: 942–947

    PubMed  CAS  Google Scholar 

  • Tomana M, Niemann M, Gardner C, Volanakis JE (1985) Carbohydrate composition of the second, third and fifth components and factors B and D of human complement. Mol Immunol 22: 107–111

    PubMed  CAS  Google Scholar 

  • Traut TW (1988) Do exons code for structural or functional units in proteins? Proc Natl Acad Sci USA 85: 2944–2948

    PubMed  CAS  Google Scholar 

  • Treisman R, Proundfoot NJ, Shander M, Maniatis T (1982) A single-base change at a splice site in a β-thalassemic gene causes abnormal RNA splicing. Cell 29: 903–911

    PubMed  CAS  Google Scholar 

  • Ueki A, Sai T, Oka H, Tabata M, Hosokawa K, Mochizuki Y (1987) Biosynthesis and secretion of the third component of complement by human endothelial cells in vitro. Immunology 61: 11–14

    PubMed  CAS  Google Scholar 

  • Warren HB, Pantazis P, Davies PF (1987) The third component of complement is transcribed; and secreted by cultured endothelial cells. Am J Pathol 129: 9–13

    PubMed  CAS  Google Scholar 

  • Wetsel RA, Lundwall A, Davidson F, Gibson T, Tack BF, Fey GH (1984) Structure of murine complement component C3. II. Nucleotide sequence of cloned complementary DNA coding for the α chain. J Biol Chem 259: 13857–13862

    PubMed  CAS  Google Scholar 

  • Wetsel RA, Ogata RT, Tack BF (1987) Primary structure of the fifth component of murine complement. Biochemistry 26: 737–743

    PubMed  CAS  Google Scholar 

  • Wetsel RA, Lemons RS, LeBeau MM, Barnum SR, Noack D, Tack BF (1988) Molecular analysis of human complement component C5: localization of the structural gene to chromosome 9. Biochemistry 27: 1474–1482

    PubMed  CAS  Google Scholar 

  • Whaley K (1980) Biosynthesis of the complement components and the regulatory proteins of the alternative complement pathway by human peripheral blood monocytes. J Exp Med 151:501–516

    PubMed  CAS  Google Scholar 

  • Wheat WH, Wetsel RA, Falus A, Tack BF, Strunk RC (1987) The fifth component of complement (C5) in the mouse. Analysis of the molecular basis for deficiency. J Exp Med 165: 1442–1447

    PubMed  CAS  Google Scholar 

  • Whitehead AS, Goldberger G, Woods DE, Markham AF, Gölten HR (1983) Use of a cDNA clone for the fourth component of human complement (C4) for analysis of a genetic deficiency of C4 in guinea pig. Proc Natl Acad Sci USA 80: 5387–5391

    PubMed  CAS  Google Scholar 

  • Whitehead AS, Sim RB, Bodner WF (1981) A monoclonal antibody against human complement component C3: the production of C3 by human cells in vitro. Eur J Immunol 11: 140–146

    PubMed  CAS  Google Scholar 

  • Whitehead AS, Solomon E, Chambers S, Bodmer WF, Povey S, Fey G (1982) Assignment of the structural gene for the third component of human complement to chromosome 19. Proc Natl Acad Sci USA 79: 5021–5025

    PubMed  CAS  Google Scholar 

  • Wieacker P, Fey G, Vorculescu I, Roper HH (1983) Exclusion of the C3 gene from the 19ql33 to 19qter region by Southern analysis of human rodent somatic cell hybrids employing a cloned genomic C3 fragment. Acta Anthropogenet 7: 107–112

    PubMed  CAS  Google Scholar 

  • Wiebauer K, Domdey H, Diggelmann H, Fey G (1982) Isolation and analysis of genomic DNA clones encoding the third component of mouse complement. Proc Natl Acad Sci USA 79: 7077–7081

    PubMed  CAS  Google Scholar 

  • Wieme RJ, Demulenaare B (1967) Genetically determined electrophoretic variant of the human complement component C’3. Nature 214: 1042–1043

    PubMed  CAS  Google Scholar 

  • Wieringa B, Meyer F, Reiser J, Weissman C (1983) Unusual splice sites revealed by mutagenic inactivation of an authentic splice site of the rabbit β-globulin gene. Nature 301: 38–43

    PubMed  CAS  Google Scholar 

  • Wieringa B, Hofer E, Weissman C (1984) A minimal intron length but no specific internal sequence is required for splicing the large rabbit β-globin intron. Cell 37: 915–925

    PubMed  CAS  Google Scholar 

  • Wright SS, Reddy PA, Jong MTC, Erickson BW (1987) C3bi receptor (complement receptor type 3) recognizes a region of complement protein C3 containing the sequence Arg-Gly-Asp. Proc Natl Acad Sci USA 84: 1965–1968

    PubMed  CAS  Google Scholar 

  • Yu CY, Belt TK, Giles CM, Campbell DR, Porter RR (1986) Structural basis of the polymorphism of human complement components C4A and C4B: gene size, reactivity and antigenicty. EMBO J 5: 2873–2881

    PubMed  CAS  Google Scholar 

  • Zimmer B, Hartung HP, Scharfenberger G, Bitter-Suermann D, Hadding U (1982) Quantitative studies of the secretion of complement component C3 by resident, elicited and activated macrophages. Comparison with C2, C4 and lysosomal enzyme release. Eur J Immunol 12: 426–430

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Dept. of Microbiology University of Alabama at Birmingham, UAB Station THT/444, Birmingham, AL, 35294, USA

    S. R. Barnum

  2. Department of Immunology, Research Institute of Scripps Clinic, La Jolla, CA, 92037, USA

    G. Fey & B. F. Tack

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  1. S. R. Barnum
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  2. G. Fey
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  3. B. F. Tack
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Editors and Affiliations

  1. Basel Institute for Immunology, Grenzacherstr. 487, CH-4005, Basel, Switzerland

    John D. Lambris Ph. D.

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Barnum, S.R., Fey, G., Tack, B.F. (1990). Biosynthesis and Genetics of C3. In: Lambris, J.D. (eds) The Third Component of Complement. Current Topics in Microbiology and Immunology, vol 153. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74977-3_2

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