Growth Requirements of B Lineage Lymphocytes From Scid and Normal Mice
Long term culture techniques provide unique approaches to dissecting components of the bone marrow microenvironment. These procedures are particularly effective for studies utilizing mice, where it is possible to selectively propagate either myeloid, or B lineage lymphoid cells for many months (Whitlock et al. 1984). Several laboratories have established and cloned “stromal” cell lines which support myelopoiesis and/or lymphopoiesis, as well as lymphocyte clones which depend on them for growth. Progress is also being made in learning which regulatory cytokines are made in these circumstances, and conditions favoring their production. However, there are indications that these model systems do not fully reflect the cellular and molecular heterogeneity that exists in vivo (Kincade et al. 1988). This has become particularly noteworthy from culture studies of genetically determined abnormalities, including SCID. Further comparison of the behavior of such defective cells in vivo and in vitro should be informative about mechanisms involved in normal lymphopoiesis.
KeywordsStromal Cell Bone Marrow Stromal Cell Severe Combine Immunodeficiency Bone Marrow Microenvironment Stromal Cell Line
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