Reconstitution of Scid Mice by Injection of Varying Numbers of Normal Fetal Liver Cells into Scid Neonates
Part of the
Current Topics in Microbiology and Immunology
book series (CT MICROBIOLOGY, volume 152)
In our original report (Bosma et al. 1983) we showed that young adult C.B-17scid/scid mice (scid mice) could be successfully engrafted with bone marrow cells of normal BALB/c donors. The reconstituted recipients readily produced serum immunoglobulin heavy (Igh) chain allotype of the donor cells, indicating that they could support the differentiation of normal lymphocytes. However, the extent of reconstitution in these and other similarly reconstituted scid mice was variable and often incomplete (Custer et al. 1985). Furthermore, the extent of reconstitution did not correlate with the number of donor cells injected (1−5 × 106 bone marrow cells) nor with the elapsed time between injection and analysis. In agreement with these findings, Fulop and Phillips (1986) reported that injection of variable numbers of normal bone marrow cells into young adult scid mice seldom resulted in normal numbers of lymphoid cells, surface Ig+ cells or colony-forming B cells. To ensure complete reconstitution, they found it necessary to irradiate scid recipients prior to cell transfer (Fulop and Phillips 1986).
KeywordsScid Mouse Severe Combine Immunodeficiency Cell Dose Fetal Liver Cell Myeloma Protein
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Bosma GC, Custer RP, Bosnia MJ (1983) A severe combined immunodeficiency mutation in the mouse. Nature (Lond) 301: 527–530CrossRefGoogle Scholar
Bosma GC, Fried M, Custer RP, Carroll A, Gibson DM, Bosnia MJ (1988) Evidence of functional lymphocytes in some (leaky) scid mice. J Exp Med 167: 1016–1033PubMedCrossRefGoogle Scholar
Custer RP, Bosma GC, Bosma MJ (1985) Severe combined immunodeficiency in the mouse. Am J Pathol 120: 464–477PubMedGoogle Scholar
Fulop GM, Phillips RA (1986) Full reconstitution of the immune deficiency in scid mice with normal stem cells requires low-dose irradiation of the recipients. J Immunol 136: 4438–4443PubMedGoogle Scholar
Gibson DM (1984) Evidence for 65 electrophoretically distinct groups of light chains in BALB/c and NZB myelomas. Mol Immunol 21: 421–432PubMedCrossRefGoogle Scholar
Litwin W (1988) Biostatistics in basic cancer research. Cancer Investigation 6 (5): 533–540PubMedCrossRefGoogle Scholar
Potter M (1972) Immunoglobulin producing tumors and myeloma proteins in mice. Physiol Rev 52: 631–719PubMedGoogle Scholar
Potter M (1977) Antigen binding proteins of mice. Adv in Immunol 25: 141–211CrossRefGoogle Scholar
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