Abnormal V(D)J Recombination in Murine Severe Combined Immune Deficiency: Absence of Coding Joints and Formation of Alternative Products
We have studied the VDJ recombination reaction in early B and T cells from mice homozygous for the severe combined immune deficiency (scid) mutation. Schuler et al. (1986) previously found abnormal deletions by Southern blot analysis at rearranged IgH and TCR β alleles in transformed B and T lymphocytes from seid mice. We have recently completed a study (Lieber et al. 1988a) in which we transfected scid lymphoid cell lines with VDJ recombination substrates that remain extrachromosomal. These substrates allow large numbers of recombinant reaction products to be collected (Hesse et al. 1987; Lieber et al. 1987), thereby permitting detailed analysis of the VDJ recombination reaction in scid lymphoid cells.
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