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Abnormal V(D)J Recombination in Murine Severe Combined Immune Deficiency: Absence of Coding Joints and Formation of Alternative Products

  • Michael R. Lieber
  • Joanne E. Hesse
  • Susanna Lewis
  • Gayle C. Bosma
  • Naomi Rosenberg
  • Kiyoshi Mizuuchi
  • Melvin J. Bosma
  • Martin Gellert
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 152)

Abstract

We have studied the VDJ recombination reaction in early B and T cells from mice homozygous for the severe combined immune deficiency (scid) mutation. Schuler et al. (1986) previously found abnormal deletions by Southern blot analysis at rearranged IgH and TCR β alleles in transformed B and T lymphocytes from seid mice. We have recently completed a study (Lieber et al. 1988a) in which we transfected scid lymphoid cell lines with VDJ recombination substrates that remain extrachromosomal. These substrates allow large numbers of recombinant reaction products to be collected (Hesse et al. 1987; Lieber et al. 1987), thereby permitting detailed analysis of the VDJ recombination reaction in scid lymphoid cells.

Keywords

Scid Mouse Severe Combine Immunodeficiency Signal Joint Severe Combine Immune Deficiency Joint Formation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1989

Authors and Affiliations

  • Michael R. Lieber
    • 1
  • Joanne E. Hesse
    • 1
  • Susanna Lewis
    • 1
  • Gayle C. Bosma
    • 2
  • Naomi Rosenberg
    • 3
  • Kiyoshi Mizuuchi
    • 1
  • Melvin J. Bosma
    • 2
  • Martin Gellert
    • 1
  1. 1.Laboratory of Molecular Biology National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaUSA
  2. 2.Fox Chase Cancer CenterInstitute for Cancer ResearchPhiladelphiaUSA
  3. 3.Department of PathologyTufts University School of MedicineBostonUSA

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