Primary Cultures of Adult Mammalian Sensory Neurons and Other in Vitro Systems of Use in Neurotoxicological Studies
There has been a significant trend in recent years, from not only within scientific and medical communities but also from a wide spectrum of social, ethical, political and commercial concerns, to seek reliable alternatives to whole animal experimentation in toxicological research. The impetus for adapting and developing methodologies to reduce, if not replace, the numbers of animals used is as great in studying substances potentially toxic to the complex nervous system as for agents to other target organs. Stringent validation is required before acceptance of in vitro methods in the predictive screening of new compounds, and this is well recognised by workers in the field (e.g. Atterwill & Steele 1987; Shahar & Goldberg 1987; Mehlman et al 1989). In vitro approaches do exist, which are capable of generating data on the mechanisms underlying acute toxicity. These permit precisely defined dosing in controlled environments, which is not possible in most in vivo studies, and are relatively quick and inexpensive to carry out. It is in mechanistic studies that cell and tissue culture can be of most value.
KeywordsDorsal Root Ganglion Dorsal Root Ganglion Neuron Cytosine Arabinoside Neural Cell Line Cytosine Arabinoside
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