Abstract
The kinetics of azide binding to chloroperoxidase (ClP) in aqueous solution and reversed micelles of sodium dodecy1sulphate (SDS), hexanol and water has been studied over a wide pH range using the Iodine Laser Temperature Jump (ILTJ) method. The pH dependence of the forward rate constant (kon) in aqueous solution eliminates several possible mechanisms and suggests that the transfer of azide to the enzyme occurs via HN3. The pH dependence of kon in the reversed micellar system suggests there is a change in the environment around the active site of the enzyme, which results in a lowering of both the limiting rate for the reaction (found at low pH’s) and the pH sensed by the enzyme. The kinetic results could be fitted to a model where ClP is located near the surface of the micelle, resulting in both a lowering of the pH around the enzyme (ca. 2 units) and a decrease in the amount of azide able to bind to the enzyme.
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Jobe, D.J., Dunford, H.B., Pickard, M., Holzwarth, J.F. (1989). Kinetics of Azide Binding to Chloroperoxidase in Water and Reversed Micelles of Sodium Dodecylsulphate, Hexanol and Water. In: Knoche, W., Schomäcker, R. (eds) Reactions in Compartmentalized Liquids. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74787-8_5
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DOI: https://doi.org/10.1007/978-3-642-74787-8_5
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