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Signal Transduction in Proliferating Normal and Transformed Cells

  • Chapter
Chemical Carcinogenesis and Mutagenesis II

Part of the book series: Handbook of Experimental Pharmacology ((HEP,volume 94 / 2))

  • 113 Accesses

Abstract

The understanding of the early events induced in cells by growth factors has been facilitated by the use of cloned cell lines that can be made quiescent by either withdrawing serum growth factors from the culture medium or by allowing the cells to grow until the available growth factors are depleted and the cells are contact-inhibited. Removal of growth factors from an exponentially growing culture does not, however, stop proliferation immediately. Those cells in or beyond late G 1, and thus committed to division, complete the cell cycle (Zetterberg and Larsson 1985). When the resulting progeny cells and the other cells in the culture enter early G1, they progress no further. The production of mRNA in these arrested cells then falls, whilst the rate of degeneration of mRNA is unchanged (Rudland et al. 1975). The cultured cells are then said to be quiescent or G0 cells; for further discussion of this transition see Whitfield et al. (1987). Following the addition of serum or defined growth factors, the quiescent cells are stimulated to enter the cell cycle. Using this experimental system, the early biochemical events occurring in the cell in response to growth stimulation can be investigated. Such studies have demonstrated several types of early change including alterations in intracellular ion concentrations, protein phosphorylation and production of second messengers.

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  1. Chester Beatty Laboratories, The Institute of Cancer Research: Royal Cancer Hospital, Fulham Road, SW3 6JB, London, Great Britain

    Colin S. Cooper Ph. D.  & Philip L. Grover D. Sc.  & 

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Wakelam, M.J.O. (1990). Signal Transduction in Proliferating Normal and Transformed Cells. In: Cooper, C.S., Grover, P.L. (eds) Chemical Carcinogenesis and Mutagenesis II. Handbook of Experimental Pharmacology, vol 94 / 2. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74778-6_14

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  • DOI: https://doi.org/10.1007/978-3-642-74778-6_14

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74780-9

  • Online ISBN: 978-3-642-74778-6

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