Signal Transduction in Proliferating Normal and Transformed Cells

  • M. J. O. Wakelam
Part of the Handbook of Experimental Pharmacology book series (HEP, volume 94 / 2)


The understanding of the early events induced in cells by growth factors has been facilitated by the use of cloned cell lines that can be made quiescent by either withdrawing serum growth factors from the culture medium or by allowing the cells to grow until the available growth factors are depleted and the cells are contact-inhibited. Removal of growth factors from an exponentially growing culture does not, however, stop proliferation immediately. Those cells in or beyond late G 1, and thus committed to division, complete the cell cycle (Zetterberg and Larsson 1985). When the resulting progeny cells and the other cells in the culture enter early G1, they progress no further. The production of mRNA in these arrested cells then falls, whilst the rate of degeneration of mRNA is unchanged (Rudland et al. 1975). The cultured cells are then said to be quiescent or G0 cells; for further discussion of this transition see Whitfield et al. (1987). Following the addition of serum or defined growth factors, the quiescent cells are stimulated to enter the cell cycle. Using this experimental system, the early biochemical events occurring in the cell in response to growth stimulation can be investigated. Such studies have demonstrated several types of early change including alterations in intracellular ion concentrations, protein phosphorylation and production of second messengers.


Inositol Phosphate Inositol Trisphosphate Oncogene Product Inositol Phospholipid Type Beta Transform Growth Factor 
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© Springer-Verlag Berlin Heidelberg 1990

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  • M. J. O. Wakelam

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