1,25-Dihydroxyvitamin D3 induziert Carboanhydrase in Knochenmarkmakrophagen

Dietsch, P.; Rossi, G.

doi:10.1007/978-3-642-74770-0_40

P. Dietsch³ &
G. Rossi³

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Summary

Besides other bone cells only osteoclasts (OC) contain the enzyme carbonic anhydrase (CA) which is needed for acid production. OC may be derived from monocytes and/or macrophages which tend to fuse under the influence of 1,25-dihydroxyvitamin D₃ (1,25(OH)₂D₃). To examine whether these multinucleated cells are OC, bone marrow macrophages were incubated in the presence of 10^-8 to 10^-6 moles/l 1,25(OH)₂D3 and CA and acid phosphatase activity were determined. Macrophages already contain some phosphatase but no CA. After 96 hours of incubation a two to three fold increase in acid phosphatase activity and CA activity of 5·10^-4 U/cell were found. These cells therefore developed a property typical for OC.

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Institut für Molekularbiologie und Biochemie, Freie Universität Berlin, Arnimallee 22, 1000, Berlin 33, Germany
P. Dietsch & G. Rossi

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P. Dietsch
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G. Rossi
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Orthopädische Klinik und Poliklinik, Georg-August-Universität, Robert-Koch-Straße 40, D-3400, Göttingen, Deutschland
Hans-Georg Willert
Radiologisches Institut, Zentrum Radiologie Katharinen-Hospital, Akad. Lehrkrankenhaus Universität Tübingen, Kriegsbergstraße 60, D-7000, Stuttgart 1, Deutschland
Friedrich H. W. Heuck (Ärztl. Direktor i.R.) (Ärztl. Direktor i.R.)

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Dietsch, P., Rossi, G. (1989). 1,25-Dihydroxyvitamin D₃ induziert Carboanhydrase in Knochenmarkmakrophagen. In: Willert, HG., Heuck, F.H.W. (eds) Neuere Ergebnisse in der Osteologie. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74770-0_40

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DOI: https://doi.org/10.1007/978-3-642-74770-0_40
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-540-51175-5
Online ISBN: 978-3-642-74770-0
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