Genetics of Capsular Polysaccharide Production in Bacteria

  • G. J. Boulnois
  • I. S. Roberts
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 150)


A feature of many bacteria of diverse genera is the production of extracellular acidic polysaccharides. These polysaccharides may be organised into distinct structures termed capsules, or may be excreted as an extracellular slime. However, this distinction is arbitrary and in practice may be of no functional significance.


Capsular Polysaccharide Neisseria Meningitidis Capsule Production Sialyl Transferase Activity Enterobacterial Common Antigen 
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  1. Aaronson W, Van W, Kotsatos M, Silver RP (1984) Identification of the gene encoding CMP-N- Acetyl neuraminic acid synthetase from the cloned K1 capsule genes of E. coli (Abstr D99). Annual Meeting of the American Society for Microbiology, WashingtonGoogle Scholar
  2. Achtman MA, Mercer A, Kusecek B, Pohl A, Heuzenroeder M, Aaronson W, Sutton A, Silver RP (1983) Six widespread bacterial clones among Escherichia coli K1 isolates. Infect Immun 39: 315–335PubMedGoogle Scholar
  3. Alexander HE, Leidy G, Hahn E (1954) Studies on the nature of Hemophilus influenzae cells susceptible to heritable changes by deoxyribonucleic acids. J Exp Med 99: 505–533PubMedCrossRefGoogle Scholar
  4. Allen PA, Roberts I, Boulnois GJ, Saunders JR, Hart CA (1987) Contribution of capsular polysaccharide and surface properties to virulence of Escherichia coli Kl. Infect Immun 55: 2662–2668PubMedGoogle Scholar
  5. Barr KK, Rick PD (1987) Biosynthesis of enterobacterial common antigen in Escherichia coli. In vitro synthesis of lipid-linked intermediates. J Biol Chem 262: 7142–7150PubMedGoogle Scholar
  6. Bayer ME (1979) The fusion sites between outer membrane and inner membrane of bacteria : their role in membrane assembly and virus infcction· In : Inouye M (Ed) Bacterial outer membranes. Wiley, New YorkGoogle Scholar
  7. Bhattacharjee AK, Jennings HJ, Kenny CP (1974) Characterisation of 3-deoxy-D-manno octulosonic acid on a component of the capsular polysaccharide antigen from Neisseria meningitidis serogroup 29E. Biochem Biophys Res Commun 61: 489–493PubMedCrossRefGoogle Scholar
  8. Bhattacharjee AK, Jennings HJ, Kenny CP, Martin A, Smith ICP (1975) Structural determination of the sialic acid polysaccharide antigens of Neisseria meningitidis serogroups B and C with carbon 13 nuclear magnetic resonance. J Biol Chem 250: 1926–1932PubMedGoogle Scholar
  9. Bhattacharjee AK, Jennings HJ, Kenny CP, Martin A, Smith ICP (1976) Structural determination of the polysaccharide antigens of Neisseria meningitidis serogroups Y, W-135 and BO. Can J Biochem 54: 1–8PubMedGoogle Scholar
  10. Boulnois GJ, Roberts IS, Hodge R, Hardy K., Jann K, Timmis KN (1987) Analysis of the K1 capsule biosynthesis genes of Escherichia coli : Definition of three functional regions for capsule production. Mol. Gen. Genet. 208: 242–246PubMedCrossRefGoogle Scholar
  11. Bundle DR, Smith ICP, Jenning HJ (1974) Determination of the structure and conformation of bacterial polysaccharides by C13 nuclear magnetic resonance : studies on the group-specific antigens of Neisseria meningitis serogroups A and X. J. Biol. Chem. 249: 2275–2281PubMedGoogle Scholar
  12. Catlin BW, Bendler JW, Goodgall SH (1972) The type b capsulation locus of Haemophilus influenzae: map location and size. J Gen Microbiol 70: 411–422PubMedGoogle Scholar
  13. Correia FF, Inouye S, Inouye M (1986) A 26 base pair repetitive sequence specific for Neisseria gonorrhoeae and Neisseria meningitidis genomic DNA. J Bacteriol 168: 1009–1015Google Scholar
  14. Echarti CE, Hirschel B, Boulnois GJ, Varley JM, Waldvogel F, Timmis KN (1983) Cloning and analysis of the K1 capsule biosynthesis genes of Escherichia coli : lack of homology with Neisseria meningitidis group B DNA sequences. Infect Immun 41: 54–60PubMedGoogle Scholar
  15. Egan W, Liu TY, Dorow D, Cohen JS, Robbins JD, Gotschlich EC, Robbins JB (1977) Structural studies on the sialic acid polysaccharide antigen of Escherichia coli strain BOS-12. Biochemistry 16: 3687–3692PubMedCrossRefGoogle Scholar
  16. Ely S, Tippett J, Kroll JS, Moxon ER (1986) Mutations affecting expression and maintenance of genes encoding the serotype b capsule of Haemophilus influenzae. J Bacteriol 167: 44–48PubMedGoogle Scholar
  17. Foulds J, Aaronson W (1984) Escherichia coli porin proteins have a role in expression of K1 capsular polysaccharide (Abstr D21). Annual Meeting of the American Society for Microbiology, Washington 1Google Scholar
  18. Frosch M, Gorgen I, Boulnois GJ, Timmis KN, Bitter-Suermann D (1985) NZB mouse system for production of monoclonal antibodies to weak bacterial antigens : Isolation of an IgG antibody to the polysaccharide capsules of Escherichia coli K1 and group B meningococci. Proc. Natl. Acad Sci USA 82: 1194–1198PubMedCrossRefGoogle Scholar
  19. Frosch M, Roberts I, Gorgen I, Metzger S, Boulnois GJ, Bitter-Suermann D (1987) Serotyping and genotyping of encapsulated Escherichia coli K1 sepsis isolates with a monoclonal IgG anti K1 antibody and K1 gene probes. Microbial Pathogen 2: 319–326CrossRefGoogle Scholar
  20. Gotschlich EC (1984) Meningococcal meningitis. In: Germanier R (ed) Bacterial vaccines. Academic, New York, pp 237–255Google Scholar
  21. Gotschlich EC, Frazer BA, Nishimura O, Robbins JB, Liu TY (1981) Lipid on capsular polysaccharides of Gram-negative bacteria. J Biol Chem 256: 8915–8921PubMedGoogle Scholar
  22. Gross RJ, Cheasty T, Rowe EJ (1977) Isolation of bacteriophages specific for the K1 polysaccharide antigen of Escherichia coli. J Clin Microbiol 6: 548–550PubMedGoogle Scholar
  23. Hoiseth SK, Connelly CJ, Moxon ER (1985) Genetics of spontaneous, high frequency loss of b capsule expression in Haemophilus influenzae. Infect. Immun. 49: 389–395Google Scholar
  24. Hoiseth SK, Moxon ER, Silver RP (1986) Genes involved in Haemophilus influenzae type b capsule expression are part of an 18-kilobase tandem duplication. Proc. Natl. Acad. Sci. USA. 83: 1106–1110PubMedCrossRefGoogle Scholar
  25. Jann K, Jann B (1982) The K antigens of Escherichia coli. Prog. Allergy 33: 53–79Google Scholar
  26. Jann K, Jann B (1984) Structure and biosynthesis of O antigens. In: Rietschel ET (ed) Handbook of endotoxin, Vol 1. Elsevier, Amsterdam, pp 138–186Google Scholar
  27. Jann K, Jann B (1985) Cell surface components and virulence : Escherichia coli O and K antigens in relation to virulence and pathogenicity. In : Sussman M (ed) The virulence of Escherichia coli. Society for General Microbiology, pp 157–176Google Scholar
  28. Kroll JS, Moxon ER (1988) Capsulation and gene Copy-number at the cap locus of Haemophilus influenzae type b. J. Bacteriol. 170: 859–864PubMedGoogle Scholar
  29. Kroll JS, Hopkins S, Moxon ER (1988) Capsule loss in H influenzae type b occurs by recombination-mediated disruption of a gene essential for polysaccharide export. Cell 53: 347–356PubMedCrossRefGoogle Scholar
  30. Kuo J S-C, Doelling VW, Graveline JF, McCoy DW (1985) Evidence for covalent attachment of phospholipid to the capsular polysaccharide of Haemophilus influenzae type b. J Bacteriol 163: 769–773PubMedGoogle Scholar
  31. Lee, C-J (1987) Bacterial capsular polysaccharides biochemistry, immunity and vaccine. Mol. Immunol. 24: 1005–1019PubMedCrossRefGoogle Scholar
  32. Lew HC, Nikaido H, Makela PH (1978) Biosynthesis of uridine diphosphate N-acetyl mannosaminuronic acid in rff mutants of Salmonella typhimurium. J Bacteriol 136: 227–233PubMedGoogle Scholar
  33. Lifely MR, Gilbert AS, Moreno C (1981) Sialic acid polysaccharide antigens of Neisseria meningitidis and Escherichia coli : esterification between adjacent residues. Carbohydr Res 94: 193–203PubMedCrossRefGoogle Scholar
  34. Liu TY, Gotschlick EC, Jonssen EK, Wysocki JR (1971 a) Studies on the meningococcal polysaccharides. I. Composition and chemical properties of the group A polysaccharide. J Biol Chem 246:2849–2858PubMedGoogle Scholar
  35. Liu T-Y, Gotschlich EC, Dunne FT, Jonssen EK (1971 b) Studies on the meningococcal polysaccharides. Composition and chemical properties of the group B and group C polysaccharides. J Biol Chem 254: 4703–4721Google Scholar
  36. Masson L, Holbein BE (1983) Physiology of sialic acid capsular polysaccharide synthesis in serogroup B. Neisseria meningitidis. J Bacteriol 154: 728–736PubMedGoogle Scholar
  37. Masson L, Holbein BE (1985) Role of lipid intermediate(s) in the synthesis of serogroup B Neisseria meningitidis capsular polysaccharide. J Bacteriol 161: 861–867PubMedGoogle Scholar
  38. Mayer H, Schmidt M (1979) Chemistry and biology of the enterobacterial common antigen (ECA). Curr Top Microbiol Immunol 85: 99–153PubMedCrossRefGoogle Scholar
  39. McGuire EJ, Binkley SB (1964) The structure and chemistry of colominic acid. Biochemistry 3: 247–251PubMedCrossRefGoogle Scholar
  40. Meier U, Mayer H (1985) Genetic location of genes encoding enterobacterial common antigen. J Bacteriol 163: 756–762PubMedGoogle Scholar
  41. Moxon ER, Deich RA, Connelly C (1984) Cloning of chromosomal DNA from Haemophilus influenzae. Its use for studying the expression of type b capsue and virulence. J Clin Invest 73: 298–306PubMedCrossRefGoogle Scholar
  42. O’Callaghan D, Maskell D, Beesley JE, Lifely MR, Roberts I, Boulnois GJ, Dougan G (1988) Characterisation and in vivo behaviour of a Salmonella typhimurium aroA strain expressing Escherichia coli K1 polysaccharide. FEMS Microbiol. Lett. 52; 269–272CrossRefGoogle Scholar
  43. Orskov F, Orskov I (1962) Behaviour of E coli antigens in sexual recombination. Acta Pathol Microbiol Scand 55:99–109CrossRefGoogle Scholar
  44. Orskov F, Orskov I, Sutton A, Schneerson R, Lind W, Egan W, Hoff GE, Robins JB (1979) Form variation in Escherichia coli K1: determined by O-acetylation of the capsular polysaccharide. J Exp Med 149: 669–685PubMedCrossRefGoogle Scholar
  45. Orskov I, Nyman K (1974) Genetic mapping of the antigenic determinants of two polysaccharide K antigens, K10 and K54, in Escherichia coli. J Bacteriol 120: 43–51PubMedGoogle Scholar
  46. Orskov I, Sharma V, Orskov F (1976) Genetic mapping of the K1 and F4 antigens (L) of Escherichia coli. Non-allelism of K(L) antigens with K antigens of 08: K27 (A), 08: K8 (L) and 09: K57 (B). Acta Pathol Microbiol Scand [B] 84: 125–131Google Scholar
  47. Orskov I, Orskov F, Jann B, Jann K (1977) Serology, chemistry and genetics of O and K antigens of Escherichia coli Bacteriol Rev 41: 667–710PubMedGoogle Scholar
  48. Paakhanen J, Gotschlick EC, Makela PH (1979) Protein K : a new major outer membrane protein found in encapsulated Escherichia coli. J Bacteriol 139: 835–841Google Scholar
  49. Rick PD, Osborn MJ (1977) Lipid A mutants of Salmonella typhimurium. J Biol Chem 252: 4895 to 4903PubMedGoogle Scholar
  50. Robbins JB, Schneerson E, Egan WB, Vann W, Liu DT (1980) Virulence properties of bacterial capsules polysaccharides — unanswered questions In: Smith H, Skehel JJ, Turner MJ (eds) The molecular basis of microbial pathogenicity. Verlag Chemie, Weinheim, pp 115–132Google Scholar
  51. Robbins JB, Schneerson R, Pittman M (1984) Haemophilus influenzae type b infection. In: Germanier R (ed) Bacterial vaccines. Academic, New York, pp 289–316Google Scholar
  52. Roberts IS, Mountford R, High N, Bitter-Suermann D, Jann K, Timmis KN, Boulnois GJ (1986) Molecular cloning and analysis of genes for production of K5, K7, K12 and K92 capsular polysaccharides in Escherichia coli. J Bacteriol 168: 1228–1233PubMedGoogle Scholar
  53. Roberts IS, Mountford R, Hodge R, Jann K, Boulnois GJ (1988) Common organisation of gene clusters for production of different capsular polysaccharides (K antigens) in Escherichia coli. J Bacteriol 170: 1305–1310PubMedGoogle Scholar
  54. Roberts M, Roberts I, Korhonen TK, Jann K, Bitter-Suermann D, Boulnois GJ, Williams PH (1988) DNA probes for K-antigen (capsule) typing of Escherichia coli. J Clin Microbiol 26 (in press)Google Scholar
  55. Rodriguez M-L, Jann B, Jann K (1988) Comparative structural elucidation of K18, K22, and K100 antigens of Escherichia coli as related ribosyl-ribitol phosphates. Carbohydr Res 173: 243–253PubMedCrossRefGoogle Scholar
  56. Schmidt MA, Jann K (1982) Phospholipid on capsular (K) polysaccharides from Escherichia coli causing extraintestinal infections. FEMS Microbiol Lett 14: 69–74CrossRefGoogle Scholar
  57. Schmidt MA, Jann K (1983) Structure of the 2-keto-3-deoxy-manno-octonic acid containing capsular polysaccharide (K12 antigen) of the urinary tract infective Escherichia coli 04: K12: H-. Eur. J. Bochem 131: 509–517CrossRefGoogle Scholar
  58. Silver RP, Finn CW, Vann WF, Aaronson W, Schneerson R, Kretschner PJ, Garon CF (1981) Molecular cloning of the K1 capsular polysaccharide genes of E. coli. Nature 289: 696–698PubMedCrossRefGoogle Scholar
  59. Silver RP, Foulds J, Todd WJ, Vann W, Aaronson W (1984 a) E. coli K1 capsule biosynthesis : studies with cloned K1 genes defective in N-acetylneuraminic acid synthesis (Abstr D100) Annual Meeting of American Society for Microbiology, WashingtonGoogle Scholar
  60. Silver RP, Vann WF, Aaronson W (1984 b) Genetic and molecular analyses of Escherichia coli K1 antigen genes. J Bacteriol 157: 568–575PubMedGoogle Scholar
  61. Silver RP, Aaronson W, Vann WF (1987) Translocation of capsular polysaccharides in pathogenic strains of Escherichia coli requires a 60-kilodalton periplasmic protein. J Bacteriol 169: 5489 to 5495PubMedGoogle Scholar
  62. Sutcliffe J, Blumenthal R, Walter A, Foulds J (1983) Escherichia coli outer membrane protein K is a porin. J Bacteriol 156: 867–872PubMedGoogle Scholar
  63. Sutherland IW (1985) Biosynthesis and composition of gram negative bacterial extracellular and wall polysaccharides. Annu. Rev. Microbiol. 39: 243–270PubMedCrossRefGoogle Scholar
  64. Timmis KN, Boulnois GJ, Bitter-Suermann D, Cabello FC (1985) Surface components of Escherichia coli that mediate resistance to the bactericidal activities of serum and phagocytes. Curr. Top Microbiol Immunol 118: 197–218PubMedCrossRefGoogle Scholar
  65. Tonn SJ, Gander JE (1979) Biosynthesis of polysaccharides by prokaryotes. Annu Rev Microbiol 33: 169–199PubMedCrossRefGoogle Scholar
  66. Troy FA (1979) The chemistry and biosynthesis of selected bacterial capsular polymers. Annu Rev Microbiol 33: 519–560PubMedCrossRefGoogle Scholar
  67. Troy FA, McCluskey MA (1979) Role of a membranous sialyltransferase complex in the synthesis of surface polymers containing polysialic acid in Escherichia coli. Temperature-induced alteration in the assembly process. J Biol Chem 254: 7377–7387PubMedGoogle Scholar
  68. Tsui F-P, Bogkin RA, Egan W (1982) Structural and immunological studies of the Escherichia coli K7 (K56) capsular polysaccharide. Carbohydr Res 102: 263–272PubMedCrossRefGoogle Scholar
  69. Tsui F-P, Egan W, Summers MF, Byrd RA (1988) Determination of the structure of the Escherichia coli K100 capsular polysaccharide, cross reactive with the capsule from type B Haemophilus influenzae. Carbohydr Res 173: 65–74PubMedCrossRefGoogle Scholar
  70. Van Alphen L, van Kampen-de Troge F, Zanen HC (1983) Characterisation of cell envelopes and lipopolysaccharides of Escherichia coli isolated from patients with neonatal meningitis. FEMS Microbiol Lett 16: 261–267CrossRefGoogle Scholar
  71. Vann WF, Schmidt MA, Jann B, Jann K (1981) The structure of the capsular polysaccharide (K5 antigen) or urinary tract infective Escherichia coli 010: K5: H4. A polymer similar to desulfo heparin. Eur J Biochem 116: 359–364PubMedCrossRefGoogle Scholar
  72. Whitfield C, Hancock REW, Costorton JW (1983) Outer membrane protein K of Escherichia coli: purification and pore-forming properties in lipid bilayer membranes. J Bacteriol 156: 873–879PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • G. J. Boulnois
    • 1
  • I. S. Roberts
    • 1
  1. 1.Department of Microbiology, Medical Sciences BuildingUniversity of LeicesterLeicesterUK

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