Advertisement

Prognosis and DNA Aneuploidy in Children with Acute Lymphoblastic Leukemia

  • M. Tsurusawa
  • N. Katano
  • T. Fujimoto
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 33)

Abstract

Childhood acute lymphoblastic leukemia (ALL) is a heterogeneous disease with respect to immunophenotype and karyotype of the blast cells, and so the biological features of the cells appear to have a strong influence on the prognosis. DNA analysis by flow cytometry (FCM) is an ideal way to detect abnormal DNA content of the leukemic cells (DNA aneuploidy), since it rapidly provides a DNA index (DI) that is independent of the mitotic index and is reliably correlated with the karyotype for DNA aneuploidy [1]. Although a favorable outcome for patients with aneuploidy has been commonly reported from our [2] and other working groups [3–5], there are some apparent differences in the prognostic value among the results of each group. In the present study we report the independent prognostic significance of the cellular DNA content in high-risk children who were stratified by WBC count and age at time of diagnosis.

Keywords

Acute Lymphoblastic Leukemia Childhood Acute Lymphoblastic Leukemia Generalize Wilcoxon Test Diploid Group 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Look AT, Melvin SL, Williams DL, Brodeur GM, Dahl GV, Kalwinsky DK, Murphy SB, Mauer M (1982) Aneuploidy and percentage of S-phase cells determined by flow cytometry correlate with cell phenotype in childhood acute leukemia. Blood 60:959–967PubMedGoogle Scholar
  2. 2.
    Tsurusawa M, Katano N, Kawai S, Fujimoto T, Maeda M (1988) Prognostic implication of cellular DNA content in acute lymphoblastic leukemia. Am J Pediatr Hematol Oncol 10:75–80PubMedCrossRefGoogle Scholar
  3. 3.
    Look AT, Roberson PK, Williams DL, Rivera G, Bowman WP, Pui CH, Ochs J, Abromowitch M, Kalwinsky D, Dahl GV, George S, Murphy SB (1985) Prognostic importance of blast cell DNA content in childhood acute lymphoblastic leukemia. Blood 65:1079–1086PubMedGoogle Scholar
  4. 4.
    Hiddemann W, Worman B, Ritter J, Thiel E, Gohde W, Lahme B, Henze G, Schellong G, Riehm H, Buchner T (1986) Frequency and clinical significance of DNA aneuploidy in acute leukemia. Ann NY Acad Sci 468:227–240PubMedCrossRefGoogle Scholar
  5. 5.
    Smets LA, Slater RM, Behrendt H, Van’t Veer B, Homan-Blok J (1985) Phenotypic and karyotypic properties of hyperdiploid acute lymphoblastic leukemia of childhood. Br J Haematol 61:113–123PubMedCrossRefGoogle Scholar
  6. 6.
    Look AT, Roberson PK, Murphy SB (1987) Prognostic value of cellular DNA content in acute lymphoblastic leukemia in childhood. N Engl J Med 317:1666PubMedGoogle Scholar
  7. 7.
    Wörmann B, Hiddemann W, Ritter J, Henze G, Langermann HJ, Kaufmann U, Schellong G, Riehm H, Buchner T (1985) Incidence and prognostic significance of DNA aneuploidy in childhood acute lymphoblastic leukemia. In:Buchner T, Bloomfield CD, Hiddemann W, Hossfeld DK, Schumann J (eds) Tumor aneuploidy. Springer, Berlin Heidelberg New York, pp 53–61Google Scholar
  8. 8.
    Beck JD, Gromball J, Klingenbiel T, Ritter J, Henze G, Riehm H, Hiddemann W (1987) DNA aneuploidy in children with relapsed acute lymphoblastic leukemia as measured by flow cytometry. Haematol Bluttransfusion 30:509–512CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • M. Tsurusawa
    • 1
  • N. Katano
    • 1
  • T. Fujimoto
    • 1
  1. 1.Japanese Children’s Cancer and Leukemia Study Group, Department of PediatricsAichi Medical UniversityAichi-gun, Aichi-kenJapan

Personalised recommendations