Blood Concentration of Ascorbyl-Free Radical in Children with Acute Lymphoblastic Leukemia:Preliminary Report

  • T. Urasiński
  • B. Gonet
  • K. Gnacińska
  • J. Peregud-Pogorzelski
  • W. Podraza
  • J. Fydryk
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 33)

Abstract

In spite of the introduction of new diagnostic tools in acute lymphoblastic leukemia (ALL), monitoring of the disease is still traditional. It is based on clinical observation and on careful evaluation of peripheral blood and bone marrow smears in search of paralymphoblastic cells. Several investigators reported that measurement of terminal deoxynucleotidyl transferase activity in extracts of isolated mononuclear cells may be a useful adjunct in monitoring therapy, but this method is not applicable to all cases of ALL [2, 5]. In a series of publications, Lohmann et al. suggested that the concentration of ascorbyl-free radical (AFR) plays an indicative and informative role in patients with ALL [10, 11]. Being inspired by his observation, we measured electron spin resonance (ESR) spectra of blood in children with ALL and healthy individuals.

Keywords

Quartz Lymphoma Leukemia Heparin DPPH 

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References

  1. 1.
    Bennett JM, Catovsky D, Daniel M-T etal. (1976) Proposals for the classification of the acute leukemias:French American British cooperative group. Br J Haematol 33:451–458PubMedCrossRefGoogle Scholar
  2. 2.
    Bollum FJ (1979) Terminal deoxynucleotidyl transferase as a hematopoietic cell marker. Blood 54:1203–1215PubMedGoogle Scholar
  3. 3.
    Cimbolaityte J J, Natkinis JJ (1982) On the nature of free radicals in dry animal tissue. Biophysica 27:800–803Google Scholar
  4. 4.
    Dodd NJ, Swartz HM (1984) The nature of the ESR signal in lyophilised tissue and its relevance to malignancy. Br J Cancer 49:65–71PubMedCrossRefGoogle Scholar
  5. 5.
    Froehlich TW, Buchanan GR, Cornet JM et al. (1981) Terminal deoxynucleotidyltrans-ferase-containing cells in peripheral blood:implications for the surveillance of patients with lymphoblastic leukemia or lymphoma in remission. Blood 58:214–220PubMedGoogle Scholar
  6. 6.
    Henze G, Langermann HJ, Fengler R et al. (1982) Therapiestudie BFM 79/81 zur Be- handlung der akuten lymphoblastischen Leukämie bei Kindern und Jugendlichen:Intensivierte Reinduktionstherapie für Patientengruppen mit unterschiedlichem Rezidivrisiko. Klin Pädiatr 194:195–203PubMedCrossRefGoogle Scholar
  7. 7.
    Mueller HW, Tannert S (1986) The significance of electron spin resonance of ascorbic acid radical in freeze dried human brain tumours and oedematous or normal periphery. Br J Cancer 53:385–391PubMedCrossRefGoogle Scholar
  8. 8.
    Neubacher H (1984) ESR investigation on lyophilised blood:mixtures with ascorbic acid. Z Naturforsch 39 C:174–176Google Scholar
  9. 9.
    Lohmann W (1981) Ascorbate oxidase and its possible involvement in cancer. Z Naturforsch 36 C:804–808Google Scholar
  10. 10.
    Lohmann W, Schreiber J, Strobelt W etal. (1979) On the possible involvement of ascorbic acid and copper proteins in leukemia:I. Electron spin resonance ( ESR) investigations on native blood, erythrocytes and leukocytes. Blut 39:317–326PubMedCrossRefGoogle Scholar
  11. 11.
    Lohmann W, Schreiber J, Gerhardt H et al. (1979) Electron spin resonance ( ESR) investigations on blood of patients with leukemia. Blut 39:147–151PubMedCrossRefGoogle Scholar
  12. 12.
    Lohmann W, Bensch KG, Sapper H et al. (1982) Free radicals and cancer. In:Mc Brien DC, Slater TF (eds) Free radicals, lipid peroxidation and cancer. Academic, London pp 55–73Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • T. Urasiński
    • 1
  • B. Gonet
    • 1
  • K. Gnacińska
    • 1
  • J. Peregud-Pogorzelski
    • 1
  • W. Podraza
    • 1
  • J. Fydryk
    • 1
  1. 1.I Pediatric Department and Department of PathophysiologyPomeranian Medical AcademySzczecinPoland

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