Complotyping and Subtyping of MHC Class I Gene Products in Haplotype Determination for Bone Marrow Transplantation
Complotyping, i.e., definition of the MHC-encoded polymorphic complement components Bf, C2, C4A, and C4B. The C4A and C4B allotypes are as polymorphic as the HLA-A and HLA-B antigens, with 12 C4A and 18 C4B variants. In addition, a duplication of one of the C4 genes is observed in 1% of the cases .
Subtyping of class I gene products by one-dimensional isoelectric focusing (1D-IEF). As we showed recently, the application of 1D-IEF followed by an immunoblotting raises the number of serologically defined antigens by nearly 60% [3, 4].
KeywordsLeukemia Recombination Electrophoresis Dition Diamine
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