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Studies of Secondary Transforming Events in Murine c-myc Retrovirus-Induced Monocyte Tumors

  • Michael R. Eccles
  • William R. Baumbach
  • Gregory D. Schuler
  • Michael D. Cole
Conference paper
Part of the Current Topics in Microbiology and Immunology book series (CT MICROBIOLOGY, volume 149)

Abstract

Rearrangement or deregulation of the c-myc gene has been observed in a variety of tumors and transformed cell lines (reviewed by Cole, 1986). Yet, the normal function or the exact contribution of c-myc in tumorigenesis remains controversial. We have recently described a tumor system in which a c-myc retrovirus induces monocyte/macrophage tumors in pristane treated BALB/c mice with very high frequency (Baumbach et al., 1986). This system has enabled us to begin dissecting the molecular changes during c-myc induced transformation of monocytes in vivo. Expression of c-myc alone is insufficient for full tumorigenicity, and as in the case of ras cooperativity (Land et al., 1983, Ruley et al., 1983), collaboration of a second genetic activation appears to be necessary. We have shown that in at least one monocyte tumor (tumor 7.1.3), a change involving the CSF-1 gene has occurred (Baumbach et al., 1987). In this tumor an endogenous ecotropic virus has integrated just upstream of the CSF-1 gene, thus activating production of CSF-1. This tumor now grows by an autocrine mechanism. Indeed all of the tumors have aquired a degree of autonomy, and so do not require CSF-1 for growth.

Keywords

Macrophage Tumor P388D1 Cell Line Genetic Event Model PDGFR Gene BMM8 Cell 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin · Heidelberg 1989

Authors and Affiliations

  • Michael R. Eccles
    • 1
  • William R. Baumbach
    • 1
  • Gregory D. Schuler
    • 1
  • Michael D. Cole
    • 1
  1. 1.Department of Molecular BiologyPrinceton UniversityPrincetonUSA

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