Purified Bovine NF-κB Recognizes Regulatory Sequences in Multiple Genes Expressed During Activation of T- and B-Lymphocytes

  • M. J. Lenardo
  • A. Kuang
  • A. Gifford
  • D. Baltimor
Conference paper
Part of the Haematology and Blood Transfusion / Hämatologie und Bluttransfusion book series (HAEMATOLOGY, volume 32)


A crucial event in the differentiation of B-lymphocytes is the transcription of the immunoglobulin light-chain gene which leads to expression of immunoglobulin antigen receptor on the surface of the cell. In an apparently separately regulated arm of the immune response, antigenic stimulation causes proliferation of T-lymphocytes by transcriptional activation of the IL-2 gene and the IL-2 receptor gene. Previous studies have shown that a lymphoid-specific enhancer element plays an important role in achieving high-level transcription of the kappa light-chain gene [1-4]. Molecular genetic dissection of this enhancer has revealed that a DNA sequence which binds to a nuclear factor, NF-κB, is essential for its function [5-8]. NF-KB binding activity is constitutively present only in mature B-lymphocytes and exceptional T-lymphocyte lines [5, 13]. Its binding may be induced in cells early in the B-lymphoid lineage, T-lymphocytes, and in nonlymphoid cells by various treatments such as bacterial lipopolysaccharide, cycloheximide, lectins, and phorbol esters [9]. Previously we have shown that NF-κB binding is critical for the kappa enhancer activity that is constitutively present in mature B-lymphocytes and inducible by lipopolysaccharide or phorbol esters in pre-B cells [7].


Phorbol Ester Mobility Shift Electrophoresis Assay Bovine Spleen Downstream Sequence Element Virus Enhancer Factor 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • M. J. Lenardo
    • 1
    • 2
  • A. Kuang
    • 1
    • 2
  • A. Gifford
    • 1
    • 2
  • D. Baltimor
    • 1
    • 2
  1. 1.Whitehead Institute for Biomedical ResearchCambridgeUSA
  2. 2.Department of BiologyMassachusetts Institute of TechnologyCambridgeUSA

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