Abstract
The general theme of the conference, “from biological activity to structure”, has prevailed in the biological research laboratory for decades. Although the methods used are now more sophisticated and the questions asked more detailed, the general approach to solving structural problems is much the same. Typically, biological activity is identified and the source and/or storage site of the material is located. Usually, an assay is developed to follow this activity through purification and isolation procedures. Chemical characterization is accomplished by a wide variety of techniques, including classical procedures as well as modern analytical methods in order to deduce the structure of the molecule responsible for this activity. It is often the case that from structure we return to biological activity in several ways. For example, this may be by chemical synthesis of the molecule in the laboratory in order to produce greater quantities of the compound at decreased cost, or by chemical modification of the original compound in order to increase its potency, to change its physical and chemical properties, or to decrease its side effects.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Barber M, Bordoli R, Elliott GJ, Sedgwick R, Tyler A ( 1982 Anal Chem 54: 645A
Day RJ, Unger SE, Cooks RG (1980) Anal Chem 52: 557A
Macfariane RD (1983) Anal Chem 55: 1247A
Kistmaker PG, vander Pyle GJ, Haverkamp J (1981) In: Morris HR (ed) Soft Ionization Biological Mass Spectrometry. Heyden, London 120
Wood GW (1982) Mass Spectrom Rev 1: 63
Cotter RJ (1980) Anal Chem 52: 1589A
Vestal ML (1983) Mass Spectrom Rev 4: 447
Cook KD (1986) Mass Spectrom Rev 4: 467
Dole M, Mack LL, Hines RL, Mobley RC, Ferguson LD, Alice MD (1968) J Chem Phys 49: 2240
Wong SS, Rollgen FW (1986) Nucl Inst and Methods in Phys Res B14: 436
Grower JL (1985) Biomed Mass Spectrom 12: 191
Lehmann WD, Kessler M, (1983) Chem Phys Lipids 32: 123
Grotjahn L, Frank R, Blocker H (1982) Nucl Acids Res 10: 4671
Lehmann WD, Kessler M, Konig vander (1984) Biomed Mass Spectrom 11: 217
Caprioli RM, Beckner CF, Smith LA (1983) Biomed Mass Spectrom 10: 94
Caprioli RM, Moore WT, Petrie G, Wilson K (1988) Int’l J Mass Spectrom Ion Phys (in press)
Ashcroft AE, Buchanan RA, Elliott GJ, Evans S, Milton DJ, Wright B, Walls FC (1988) Proceedings of the 36th ASMS Conf on Mass Spectrom, June 5–10, San Francisco 1156
Hillenkamp F (1988) presented at the 11th Int’l Conference on Mass Spectrometry, Bordeaux, Aug 29–Sept 2
Johnson JV, Lee MS, Lee MR, Brotherton HO, Yost RA (1986) In: Gaskell SJ (ed) Mass Spectrometry in Biomed Res. Wiley, New York, 459
Fenselau C, Yergey J, Heller D (1983) Int’l J Mass Spectrom Ion Phys 53: 5
Caprioli RM (1983) Anal Chem 55: 2387
Beckner CF, Caprioli RM (1983) Anal Bchm 130: 328
Beckner CF, Caprioli RM (1984) Biomed Mass Spectrom 11: 60
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Caprioli, R.M. (1989). FAB: Basic Concepts and Practical Considerations. In: Schlunegger, U.P. (eds) Biologically Active Molecules. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74582-9_4
Download citation
DOI: https://doi.org/10.1007/978-3-642-74582-9_4
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-74584-3
Online ISBN: 978-3-642-74582-9
eBook Packages: Springer Book Archive