Abstract
The adenovirus terminal protein (TP) is found covalently attached to the 5’-ends of viral DNA molecules (Robinson et al. 1973; Robinson and Bellett 1974; Rekosh et al. 1977). The linkage is through a phosphodiester bond formed between the ß-hydroxyl group of a serine residue within the C-terminal domain of the TP and the 5’-hydroxyl of the terminal deoxycytosine residue on the viral DNA (DesI-derIo and Kelly 1981; Smart and Stillman 1982). The protein initially attached to the viral chromosome is termed the preterminal protein (pTP), and it is cleaved to form the TP during virion maturation (Challberg and Kelly 1981; Stillmann et al. 1981). The functional significance of the cleavage is unclear.
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References
Challberg MD, Kella TJ (1981) Processing of the adenovirus terminal protein. J Virol 38: 272–277
Cook PR, Lang J, Hayday A, Lania L, Fried M, Chiswell DJ, Wyke JA (1982) Active viral genes in transformed cells lie close to the nuclear cage. EMBO J 4: 447–452
Desiderio SV, Kelly TJ (1981) The structure of the linkage between adenovirus DNA and the 55,000 dalton terminal protein. J Mol Biol 145: 319–337
Freimuth PI, Ginsberg HS (1986) Codon insertion mutants of the adenovirus terminal protein. Proc Natl Acad Sci USA 83: 7816–7820
Hearing P, Shenk T (1985) Sequence-independent autoregulation of the adenovirus type 5 ElA transcription unit. Mol Cell Biol 5: 3214–3221
Jackson DA, McCready SJ, Cook PR (1981) RNA is synthesized at the nuclear cage. Nature 292: 552–555
Jones N, Shenk T (1979) Isolation of Ad5 host range deletion mutants defective for transformation of rat embryo cells. Cell 17: 683–689
Kelly TJ (1984) Adenovirus DNA replication. In: Ginsberg H (ed) The Adenoviruses. Plenum, New York, pp 271–307
Melton DA, Krieg PA, Rebagliati MR, Maniatis T, Zinn K, Green MR (1984) Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. Nucleic Acids Res 12: 7035–7056
Mirkovitch J, Mirault ME, Laemmli UK (1984) Organization of the higher-order chromatin loop: specific DNA attachment sites on nuclear scaffold. Cell 39: 223–232
Nelkin BD, Pardoll DM, Vogelstein B (1980) Mapping sequences in loops of nuclear DNA by their progressive detachment from the nuclear cage. Nucleic Acids Res 8: 2895–2908
Nelson WG, Plenta KJ, Barrack ER, Coffey DS (1986) The role of the nuclear matrix in the organization and function of DNA. Annu Rev Biophys Biophys Chem 15: 457–475
Pardoll DM, Vogelstein B (1980) Sequence analysis of nuclear matrix associated DNA from rat liver. Exp Cell Res 128: 466 470
Rekosh DMK, Russell WC, Bellet AJD, Robinson AJ (1977) Identification of a protein linked to the ends of adenovirus DNA. Cell 11: 283–295
Robinson AJ, Bellett AJD (1974) A circular DNA-protein complex from adenoviruses and its possible role in DNA replication. Cold Spring Harbor Symp Quant Biol 39: 523–531
Robinson AJ, Younghusband HB, Bellett AJD (1973) A circular DNA-protein complex from adenoviruses. Virology 56: 54–69
Robinson SI, Small D, Idzerda R, McKnight GS, Vogelstein B (1983) The association of transcriptonally active genes with the nuclear matrix of the chicken oviduct. Nucleic Acids Res 11:5113–5130
Smart JE, Stilman BW (1982) Adenovirus terminal protein precursor: partial amino acid sequence and the site of covalent linkage to virus DNA. J Biol Chem 257: 13499–13506
Stillman B (1983) The replication of adenovirus DNA with purified proteins. Cell 35: 7–9
Stillman BW, Lewis JB, Chow LT, Mathews MB, Smart JE (1981) Identification of the Gene and mRNA for the adenovirus terminal protein precursor. Cell 23: 497–508
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© 1989 Springer-Verlag Berlin · Heidelberg
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Schaack, J., Shenk, T. (1989). Adenovirus Terminal Protein Mediates Efficient and Timely Activation of Viral Transcription. In: Knippers, R., Levine, A.J. (eds) Transforming Proteins of DNA Tumor Viruses. Current Topics in Microbiology and Immunology, vol 144. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74578-2_23
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DOI: https://doi.org/10.1007/978-3-642-74578-2_23
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