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Sperm-Activating Peptides from Sea Urchin Egg Jelly

  • Norio Suzuki
Part of the Bioorganic Marine Chemistry book series (BMC, volume 3)

Abstract

Speract (Gly-Phe-Asp-Leu-Asn-Gly-Gly-Gly-Val-Gly) and ten of its derivatives obtained from the egg jelly of sea urchins, Order Echinoida, stimulate the respiration and motility of spermatozoa of sea urchins belonging to the same order. Resact (Cys-Val-Thr-Gly-Ala-Pro-Gly-Cys-Val-Gly-Gly-Gly-Arg-LeuNH2) and mosact (Asp-Ser-Asp-Ser-Ala-Gln-Asn-Leu-Ile-Gly) and two of its derivatives obtained from Arbacia punctulata eggs, Order Arbacioida, and Clypeaster japonicus eggs, Order Clypeasteroida, also stimulate the metabolism and motility of respective sea urchin spermatozoa. Two other peptides (Gly-(X)-Pro-(X)-Gly-Gly-Ala-Val- and Open image in new window ) were isolated from the egg jelly of the sea urchin Diadema setosum, Order Diadematoida. These are specific for Diadema setosum spermatozoa. Early biochemical responses of the spermatozoa to the egg jelly peptides include at a net hydrogen ion efflux and elevations of cyclic AMP and cyclic GMP concentrations. In additoin, a major sperm plasma protein is modified in response to the peptides such that its apparent molecular weight is lowered with exception of the response of Glyptocidaris crenularis spermatozoa to alloresact (Lys-Leu-Cys-Pro-Gly-Gly-Asn-Cys-Val). The plasma membrane protein appears to be the enzyme guanylate cyclase. Resact acts as a chemoattractant for Arbacia punctulata and speract promotes the acrosome reaction of Hemicentrotus pulcherrimus spermatozoa as a cofactor.

Keywords

Guanylate Cyclase Acrosome Reaction Jelly Layer Respiratory Stimulation Hemicentrotus Pulcherrimus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Norio Suzuki
    • 1
  1. 1.Noto Marine LaboratoryKanazawa UniversityOgi, Uchiura, IshikawaJapan

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