Initiation and Characterization of Photoautotrophic Suspension Cultures

  • J. M. Widholm


A review of the literature shows that photoautotrophic cultures of the following 16 different species have been established: Nicotiana tabacum (Bergmann 1967, 1968), Ruta graveolens (Corduan 1970), Chenopodium rubrum (Husemann and Barz 1977), Cytisus scoparius (Yamada and Sato 1978), Peganum harmala (Barz et al. 1980), Spinacia oleracea (Dalton 1980), Datura stramonium (Yasuda et al. 1980), Hyoscyamus niger (Yasuda et al. 1980), Arachis hypogea (Bender et al. 1981), Asparagus officinalis (Peel 1982), Marchantia polymorpha (Katoh 1983), Glycine max (Horn et al. 1983b), Solanum tuberosum (LaRosa et al. 1984), Morinda lucida (Igbavboa et al. 1985), Catharanthus roseus (Tyler et al. 1986), and Euphorbia characias (Hardy et al. 1987). While this number (16) is not small it should be pointed out that the establishment of photoautotrophic cultures which are convincingly photoautotrophic, i.e., cultures which can be grown for many subcultures in medium without sugar or other energy sources, is usually a time-consuming and often difficult task. Several of the cultures listed above are grown on agar-containing medium which can complicate the definition since McHale (1985) has shown that agar or agarose-containing medium without sucrose can support the growth of green Nicotiana tabacum cells incubated in light in CO2-free air.


Suspension Culture Dark Respiration Soybean Line Dark Respiration Rate Photoautotrophic Growth 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • J. M. Widholm
    • 1
  1. 1.Department of AgronomyUniversity of IllinoisUrbanaUSA

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