Development of Genetic and Analytical Systems for Studies of Auxin Metabolism
Early investigations of auxin conjugates concerned the general “release” of auxin in vivo or in situ [e.g., 8]. More recent studies have examined either IAA released by hydrolysis of extracts of plant tissue, or have studied specific conjugates formed by the covalent attachment of IAA to other molecules. Conjugated forms of IAA can be classified by size, type of covalent linkage, or the molecule to which the IAA is attached. Low molecular weight conjugates include esters such as IAA-glucose and IAA-myo-inositol and amides such as IAA-aspartate and IAA-glutamate. The higher molecular weight conjugates include esters where the IAA is linked to the carbohydrate portion of a glycoprotein , or is linked to a glucan . Higher molecular weight amide conjugates are also known, where IAA is linked directly to a peptide or protein . These higher molecular weight conjugates have been difficult to study due to the lack of suitable methods for macromolecular separations and structure determination. Improvements in available methods for studies of macromolecules now make it practical to examine these types of compounds in more detail and begin to ask questions as to their role in the hormonal relationships within the plant.
KeywordsHydrolysis HPLC Acetonitrile Amide Carboxyl
Unable to display preview. Download preview PDF.
- 5.Chang W-C, Chiu P-L (1978) Z Pflanzenphysiol 89:91Google Scholar
- 6.Chen K-H (1987) Analysis of indole-3-acetic acid in tobacco genetic tumors and in wheat GA3 insensitive mutant “Tom Thumb”, Ph D Thesis. University of Maryland, College Park, pp 130Google Scholar
- 13.Cohen JD, Slovin JP, Bialek K (1985) Kienph In: Randall DD, Blevins DG, Larson RL (eds) Current topics in plant biochemistry and physiology 1985, vol 4. University of Missouri-Columbia, p 75Google Scholar
- 19.Rivier L (1986) Kienph In: Linskens HF, Jackson JF (eds) Modern methods of plant analysis, new series. Vol 3 Gas Chromatography/Mass Spectrometry, Springer, Berlin Heidelberg New York Tokyo, p 146Google Scholar
- 20.Sandberg G, Crozier A, Ernstsen A (1987) Kienph In: Rivier L, Crozier A (eds) Principles and practice of plant hormone analysis. Academic Press, London, p 169Google Scholar
- 21.Schoken V (1949) Arch Biochem 23:198Google Scholar
- 22.Slovin JP, Cohen JD (1985) (Abstract) Plant Physiol 77:S-11Google Scholar
- 23.Slovin JP, Cohen JD (1986) (Abstract) Plant Physiol 80:S-33Google Scholar