Development of Genetic and Analytical Systems for Studies of Auxin Metabolism

  • J. D. Cohen
  • K. Bialek
  • J. P. Slovin
  • B. G. Baldi
  • K.-H. Chen
Conference paper


Early investigations of auxin conjugates concerned the general “release” of auxin in vivo or in situ [e.g., 8]. More recent studies have examined either IAA released by hydrolysis of extracts of plant tissue, or have studied specific conjugates formed by the covalent attachment of IAA to other molecules. Conjugated forms of IAA can be classified by size, type of covalent linkage, or the molecule to which the IAA is attached. Low molecular weight conjugates include esters such as IAA-glucose and IAA-myo-inositol and amides such as IAA-aspartate and IAA-glutamate. The higher molecular weight conjugates include esters where the IAA is linked to the carbohydrate portion of a glycoprotein [17], or is linked to a glucan [18]. Higher molecular weight amide conjugates are also known, where IAA is linked directly to a peptide or protein [3]. These higher molecular weight conjugates have been difficult to study due to the lack of suitable methods for macromolecular separations and structure determination. Improvements in available methods for studies of macromolecules now make it practical to examine these types of compounds in more detail and begin to ask questions as to their role in the hormonal relationships within the plant.


Bean Seed Isotope Dilution Technique Auxin Metabolism Wheat Caryopsis Amide Conjugate 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • J. D. Cohen
    • 1
  • K. Bialek
    • 1
  • J. P. Slovin
    • 1
    • 2
  • B. G. Baldi
    • 1
  • K.-H. Chen
    • 2
  1. 1.USDA-ARS Plant Hormone LaboratoryBeltsville Agricultural Research CenterBeltsvilleUSA
  2. 2.Department of BotanyUniversity of MarylandCollege ParkUSA

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