Abstract
The pathways and cofactor requirements for the conversion of GA12-aldehyde to C20- and C19-GAs in cell-free systems from different objects are now well known and the purification of the enzymes contained in these systems has begun. In this part of the pathway, the C-20 carbon atom is oxidized step by step and finally lost as CO2 to yield the γ-lactone typical for C19-GAs. The GA molecule may further become modified by hydroxylations, which profoundly influence its physiological activity. In general, 3β-hydroxylation increases the activity strongly — it may even be essential for activity — whereas 2β-hydroxylation inactivates the GA. The relative rates of C19-GA biosynthesis, 3β-hydroxylation and 2β-hydroxylation determine the amounts of physiologically active GA available to the plant, which explains the interest in the corresponding enzymes and their regulation. The GA biosynthesis pathways have recently been reviewed [4, 5, 9].
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Abbreviations
- BSA:
-
bovine serum albumin
- DTE:
-
dithioerythritol
- DTT:
-
dithiothreitol
- EDTA:
-
ethylenediaminetetraacetic acid
- GA(s):
-
gibberellins(s)
- GAn :
-
gibberellin An
- HPLC:
-
high performance liquid chromatography
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Graebe, J.E., Lange, T. (1990). The Dioxygenases in Gibberellin Biosynthesis after Gibberellin A12-Aldehyde. In: Pharis, R.P., Rood, S.B. (eds) Plant Growth Substances 1988. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74545-4_37
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DOI: https://doi.org/10.1007/978-3-642-74545-4_37
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