Abstract
The existence of a system transporting pyruvic acid through the inner mitochondrial membrane has been for a long time a matter of debate. In 1971, when such a system was described for the first time (Paradies et al, 1971) and since then it has been characterized in terms of its substrate specificity. Both other 2-oxo acids and their derivatives substituted in position 2 with halogens turned out to be good and competitive substrates in the reactions of either net pyruvate uptake according to the transmembrane pH difference or in the exchange reaction (Halestrap, 1975; Paradies & Papa, 1977). Halestrap described a series of chemical compounds which exhibited an inhibitory effect on the pyruvate transport measured in intact mitochondria, among them the most widely applied being α-cyanocinnamate and its 4-hydroxy derivative (Halestrap, 1975).
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© 1989 Springer-Verlag Berlin Heidelberg
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Nałçcz, K.A., Bolli, R., Wojtczak, L., Azzi, A. (1989). Purification of the Monocarboxylate Carrier by Affinity Chromatography. In: Azzi, A., Nałęz, K.A., Nałęcz, M.J., Wojtczak, L. (eds) Anion Carriers of Mitochondrial Membranes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74539-3_4
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DOI: https://doi.org/10.1007/978-3-642-74539-3_4
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