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Purification of the Monocarboxylate Carrier by Affinity Chromatography

  • Katarzyna A. Nałçcz
  • Reinhard Bolli
  • Lech Wojtczak
  • Angelo Azzi
Conference paper

Abstract

The existence of a system transporting pyruvic acid through the inner mitochondrial membrane has been for a long time a matter of debate. In 1971, when such a system was described for the first time (Paradies et al, 1971) and since then it has been characterized in terms of its substrate specificity. Both other 2-oxo acids and their derivatives substituted in position 2 with halogens turned out to be good and competitive substrates in the reactions of either net pyruvate uptake according to the transmembrane pH difference or in the exchange reaction (Halestrap, 1975; Paradies & Papa, 1977). Halestrap described a series of chemical compounds which exhibited an inhibitory effect on the pyruvate transport measured in intact mitochondria, among them the most widely applied being α-cyanocinnamate and its 4-hydroxy derivative (Halestrap, 1975).

Keywords

Affinity Chromatography Heart Mitochondrion Affinity Resin Adenine Nucleotide Transporter Triton Extract 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Katarzyna A. Nałçcz
    • 1
    • 2
  • Reinhard Bolli
    • 1
    • 2
  • Lech Wojtczak
    • 1
    • 2
  • Angelo Azzi
    • 1
    • 2
  1. 1.Department of Cellular BiochemistryNencki Institute of Experimental BiologyWarsawPoland
  2. 2.Institut für Biochemie und MolekularbiologieUniversität BernBernSwitzerland

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