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A Molecular Biology Study of the Uncoupling Protein of Brown Fat Mitochondria. A Contribution to the Analysis of Genes of Mitochondrial Carriers

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Anion Carriers of Mitochondrial Membranes

Abstract

The uncoupling protein (UCP) is a specialized mitochondrial carrier unique to brown adipose tissue mitochondria. It acts as a proton carrier able to dissipate the proton gradient, to bypass ATP synthesis and to dissipate energy as heat (Nicholls & Locke, 1984). The proton translocating activity of UCP is inhibited by di- and triphosphate purine nucleotides and activated by free fatty acids (Nicholls et al., 1986). Moreover, UCP can be used as a marker to identify thermogenic adipocytes.

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References

  • Aquila H, Link TA, Klingenberg M. (1985) The uncoupling protein from brown fat mitochondria is related to the mitochondrial ADP/ATP carrier. Analysis of sequence homologies and of folding of the protein in the membrane. EMBO J 4:2369–2376

    PubMed  CAS  Google Scholar 

  • Aquila H, Link TA, Klingenberg M (1987) Solute carriers involved in energy transfer of mitochondria form a homologous protein family. FEBS Lett 212:1–9

    Article  PubMed  CAS  Google Scholar 

  • Aquila H, Misra D, Eulitz M, Klingenberg M (1982) Complete amino-acid sequence of the ADP/ATP carrier from beef heart mitochondria. Hoppe-Seyler’s Z Physiol Chem 363:345–349

    Article  PubMed  CAS  Google Scholar 

  • Battini R, Ferrari S, Kaczmarek L, Calabretta B, Sing-Tsueng C, Baserga R (1987) Molecular cloning of a cDNA from a human ADP/ATP carrier which is growth-regulated. J Biol Chem 262:4355–4359

    PubMed  CAS  Google Scholar 

  • Bouillaud F, Raimbault S, Ricquier D (1988b) Complete sequence of the gene of rat uncoupling protein (in preparation)

    Google Scholar 

  • Bouillaud F, Ricquier D, Thibault J, Weissenbach J (1985) Molecular approach to thermogenesis in brown adipose tissue: cDNA cloning of the mitochondrial uncoupling protein. Proc Natl Acad Sci (USA) 82:445–448

    Article  CAS  Google Scholar 

  • Bouillaud F, Villarroya F, Hentz E, Raimbault S, Cassard AM, Ricquier D (1988a) Detection of brown adipose tissue uncoupling protein mRNA in adult patients by a human genomic probe. Clin Sci 74 (in press)

    Google Scholar 

  • Bouillaud F, Weissenbach J, Ricquier D (1986) Complete cDNA-derived amino-acid sequence of rat brown fat uncoupling protein. J Biol Chem 261:1487–1490

    PubMed  CAS  Google Scholar 

  • Casteilla L, Bouillaud F, Forest C, Ricquier D (1988a) Molecular cloning of bovine and ovine brown fat uncoupling protein. Structural and evolutionary relationship to bovine adenine nucleotide translocator and mitochondrial phosphate carrier (submitted)

    Google Scholar 

  • Casteilla L, Champigny O, Bouillaud F, Robelin J, Ricquier D (1988b) Sequential changes in the expression of mitochondrial protein mRNA during the development of brown adipose tissue in bovine and ovine species. Biochem J (in press)

    Google Scholar 

  • Dalbon P, Brandolin G, Boulay F, Höppe J, Vignais PV (1988) Mapping of the nucleotide binding sites in the ADP/ATP carrier of beef heart mitochondria by photolabeling with 2-azido [-32P] adenosine diphosphate. Biochemistry 27:5141–5149

    Article  PubMed  CAS  Google Scholar 

  • Freeman KB, Chien SM, Litchfield D, Patel HV (1983) Synthesis in vitro of rat brown adipose tissue 32,000 Mr protein. FEBS Lett 158:325–330

    Article  PubMed  CAS  Google Scholar 

  • Houldsworth J, Attardi G (1988) Two distinct genes for ADP/ATP translocase are expressed at the mRNA level in adult human liver. Proc Natl Acad Sci (USA) 85:377–381

    Article  CAS  Google Scholar 

  • Jacobsson A, Stadler U, Glotzer MA, Kozak LP (1985) Mitochondrial uncoupling from mouse brown fat. Molecular cloning, genetic mapping & mRNA expression. J Biol Chem 260:16250–16254

    PubMed  CAS  Google Scholar 

  • Kolbe HVJ, Wohlrab H (1985) Sequence of N-terminal formic acid fragment and location of the N-ethylmaleimide-binding site of the phosphate transport protein from beef heart mitochondria. J Biol Chem 29:15899–15906

    Google Scholar 

  • Mory G, Bouillaud F, Combes-George M, Ricquier D (1984) Noradrenaline controls the concentration of the uncoupling protein in brown adipose tissue. FEBS Lett 166:393–396

    Article  PubMed  CAS  Google Scholar 

  • Neckelman N, Kang L, Wade RP, Shuster R, Wallace DL (1987) cDNA sequence of a human skeletal muscle ADP/ATP translocator: lack of a leader peptide, divergence from a fibroblast translocator cDNA, and co-evolution with mitochondrial DNA genes. Proc Natl Acad Sci (USA) 84:7580–7584

    Article  Google Scholar 

  • Nicholls DG, Cunningham S, Rial E (1986) The bioenergetic mechanisms of brown adipose tissue. In: Trayhurn P, Nicholls DG (eds) Brown Adipose Tissue. Edward Arnold, London, p 52

    Google Scholar 

  • Nicholls DG, Locke R (1984) Thermogenic mechanisms in brown fat. Physiol Rev 64:1–64

    PubMed  CAS  Google Scholar 

  • Rasmussen UB, Wohlrab H (1986) Bovine cardiac ADP/ATP carrier: two distinct mRNAs and on unusually short 3′-non coding sequence. Biochem Biophys Res Commun 138:850–857

    Article  PubMed  CAS  Google Scholar 

  • Ricquier D, Bouillaud F, Toumelin P, Mory G, Bazin R, Arch J, Pénicaud L (1986) Expression of uncoupling protein mRNA in thermogenic or weakly thermogenic brown adipose tissue. J Biol Chem 261:13905–13910

    PubMed  CAS  Google Scholar 

  • Ricquier D, Mory G (1984) Factors affecting brown adipose tissue activity in animals and man. Clin Endocrinol Metab 13:503–519

    Article  Google Scholar 

  • Ricquier D, Mory G, Bouillaud F, Thibault J, Weissenbach J (1984) Rapid increase mitochondrial uncoupling protein and its mRNA in stimulated brown adipose tissue. Use of a cDNA probe. FEBS Lett 178:240–244

    Article  CAS  Google Scholar 

  • Ricquier D, Thibault J, Bouillaud F, Kuster Y (1983) Molecular approach to thermogenesis in brown adipose tissue. Cell-free translation of mRNA and characterization of the mitochondrial uncoupling protein. J Biol Chem 258:6675–6677

    PubMed  CAS  Google Scholar 

  • Ridley RG, Patel HV, Gerber GE, Morton RC, Freeman KB (1986) Complete nucleotide sequence and derived amino-acid sequence of cDNA encoding the mitochondrial uncoupling protein of rat brown adipose tissue: lack of mitochondrial targeting pre-sequence. Nucl Ac Res 14:4025–4035

    Article  CAS  Google Scholar 

  • Runswick RJ, Powell SJ, Nyren P, Walker JE (1987) Sequence of the bovine mitochondrial phosphate carrier protein: structural relationship to ADP/ATP translocase and the brown fat mitochondrial uncoupling protein. EMBO J 6:1367–1373

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Centre National de la Recherche Scientifique, Centre de Recherche sur la Nutrition, 9 rue Jules Hetzel, F-92190, Meudon-Bellevue, France

    Frederic Bouillaud, Serge Raimbault, Louis Casteilla, Anne-Marie Cassard & Daniel Ricquier

Authors
  1. Frederic Bouillaud
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  2. Serge Raimbault
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  3. Louis Casteilla
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  4. Anne-Marie Cassard
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  5. Daniel Ricquier
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Editor information

Editors and Affiliations

  1. Institut für Biochemie und Molekularbiologie, Universität Bern, Bühlstraße 28, CH-3012, Bern, Switzerland

    Angelo Azzi

  2. Department of Cellular Biochemistry, Nencki Institute of Experimental Biology, Pasteurstreet 3, PL-02-093, Warsaw, Poland

    Katarzyna A. Nałęz , Maciej J. Nałęcz  & Lech Wojtczak ,  & 

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© 1989 Springer-Verlag Berlin Heidelberg

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Bouillaud, F., Raimbault, S., Casteilla, L., Cassard, AM., Ricquier, D. (1989). A Molecular Biology Study of the Uncoupling Protein of Brown Fat Mitochondria. A Contribution to the Analysis of Genes of Mitochondrial Carriers. In: Azzi, A., Nałęz, K.A., Nałęcz, M.J., Wojtczak, L. (eds) Anion Carriers of Mitochondrial Membranes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74539-3_20

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  • DOI: https://doi.org/10.1007/978-3-642-74539-3_20

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74541-6

  • Online ISBN: 978-3-642-74539-3

  • eBook Packages: Springer Book Archive

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