Abstract
The inner mitochondrial membrane contains a specific 2-oxoglutarate transporter which catalyzes the uptake of 2-oxoglutarate by an electroneutral counter-exchange with 2-oxoglutarate or L-malate. This antiport system accepts also other dicarboxylates as oxaloacetate, succinate and malonate (for review see LaNoue & Schoolwerth, 1979).
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References
Bisaccia F, Palmieri F (1984) Specific elution from hydroxylapatite of the mitochondrial phosphate carrier by cardiolipin. Biochim Biophys Acta 766:386–394
Bisaccia F, Indiveri C, Palmieri F (1985) Purification of reconstitutively active 2-oxoglutarate carrier from pig heart mitochondria. Biochim Biophys Acta 810:362–369
Bisaccia F, Indiveri C, Palmieri F (1988) Purification and reconstitution of two anion carriers from rat liver mitochondria: the dicarboxylate and the 2-oxoglutarate carrier. Biochim Biophys Acta 933:229–240
Cheetham PSJ (1979) Removal of Triton X-100 from aqueous solution using Amberlite XAD-2. Anal Biochem 92:447–452
de Pinto V, Prezioso G, Palmieri F (1987) A simple and rapid method for the purification of the mitochondrial porin from mammalian tissues. Biochim Biophys Acta 905:499–502
Halestrap AP (1975) The mitochondrial pyruvate carrier. Kinetics and specificity for substrates and inhibitors. Biochem J 148:85–96
Houštek J, Pedersen PL (1985) Adenine nucleotide and phosphate transport systems of mitochondria. Relative location of sulfhydryl groups based on the use of the novel fluorescent probe eosin-5-maleimide. J Biol Chem 260:6288–6295
Indiveri C, Krämer R, Palmieri F (1987) Reconstitution of the malate/aspartate shuttle from mitochondria. J Biol Chem 262:15979–15983
Indiveri C, Palmieri F, Bisaccia F, Krämer R (1987) Kinetics of the reconstituted 2-oxoglutarate carrier from bovine heart mitochondria. Biochim Biophys Acta 890:310–318
Johnson D, Lardy H (1967) Isolation of liver or kidney mitochondria. Methods Enzymol 10:94–96
Krämer R (1982) Cholesterol as activator of ADP-ATP exchange in reconstituted liposomes and in mitochondria. Biochim Biophys Acta 693:296–304
Krämer R, Heberger C (1986) Functional reconstitution of carrier proteins by removal of detergent with a hydrophobic ion exchange column. Biochim Biophys Acta 863:289–296
LaNoue KF, Schoolwerth AC (1979) Metabolite transport in mitochondria. Ann Rev Biochem 48:871–922
Lowry OH, Rosenbrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin Phenol reagent. J Biol Chem 193:265–275
Meijer AJ, Van Woerkom GM, Eggelte TA (1976) Phthalonic acid; an inhibitor of 2-oxoglutarate transport in mitochondria. Biochim Biophys Acta 430:53–61
Meijer AJ, Van Dam K (1981) Mitochondrial transport: In: Boting S, De Pont J (eds) Membrane Transport. Elseyier Amsterdam, pp 235-256
Müller M (1983) Lipid-protein and protein-protein interactions in the inner mitochondrial membrane. PhD thesis ETH nr 7392
Müller M, Cheneval D, Carafoli E (1984) Doxorubicin inhibits the phosphate-transport protein reconstituted in liposomes. A study on the mechanism of the inhibition. Eur J Biochem 140:447–452
Müller M, Krebs JJR, Cherry RJ, Kawato S (1984) Rotational diffusion of the ADP/ATP translocator in the inner membrane of mitochondria and in proteoliposomes. J Biol Chem 259:3037–3043
Nałęcz MJ (1986) Metabolite transport systems of inner mitochondrial membrane: characteristics and isolation attempts. In: Kuczera J, Przestalski S (eds) Biophysics of Membrane Transport, vol.2. Publ. Dept. of the Agricultural University of Wroclaw, pp 13-45
Palmieri F, Quagliariello E, Klingenberg M (1972) Kinetics and specificity of the oxoglutarate carrier in rat-liver mitochondria. Eur J Biochem 29:408–416
Quagliariello E, Palmieri F (1972) Kinetics of substrate uptake by mitochondria. Identification of carrier sites for substrates and inhibitors. In: Azzone GF, Carafoli E, Lehninger AL, Quagliariello E, Siliprandi N (eds) Biochemistry and Biophysics of Mitochondrial Membranes. Academic Press New York and London, pp 659-680
Rigaud J, Paternostre M, Bluzat A (1988) Mechanisms of membrane protein insertion into liposomes during reconstitution procedures involving the use of detergents. 2. Incorporation of the light-driven proton pump bacteriorhodopsin. Biochemistry 27:2677–2688
Sluse FE, Ranson M, Liebecq C (1972) Mechanism of the exchanges catalysed by the oxo-glutarate translocator of rat-heart mitochondria. Eur J Biochem 25:207–217
Sluse FE, Goffart G, Liebecq C (1973) Mechanism of the exchanges catalysed by the oxoglutarate translocator of rat-heart mitochondria. Kinetics of the external-product inhibition. EUT J Biochem 32:283–291
Sluse-Goffart CM, Sluse FE, Duyckaerts C, Richard M, Hengesch P, Liebecq C (1983) Conformational changes and possible structure of the oxoglutarate translocator of rat-heart mitochondria revealed by the kinetic study of malate and oxoglutarate uptake. Eur J Biochem 134:397–406
Stipam I, Zara V, Zaki L, Prezioso G, Palmieri F (1986) Inhibition of the mitochondrial tricarboxylate carrier by arginine-specific reagents. FEBS Lett 205:282–286
Ueno M, Tanford C, Reynolds A (1984) Phospholipid vesicle formation using nonionic detergents with low monomer solubility. Kinetic factors determine vesicle size and permeability. Biochemistry 23:3070–3076
Yu ChA, Yu L, King TE (1975) Studies on cytochrome oxidase. Interactions of the cytochrome oxidase protein with phospholipids and cytochrome c. J Biol Chem 350:1383–1392
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© 1989 Springer-Verlag Berlin Heidelberg
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Claeys, D., Müller, M., Azzi, A. (1989). Purification and Reconstitution of the 2-Oxoglutarate Carrier from Bovine Heart and Liver Mitochondria. In: Azzi, A., Nałęz, K.A., Nałęcz, M.J., Wojtczak, L. (eds) Anion Carriers of Mitochondrial Membranes. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74539-3_2
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DOI: https://doi.org/10.1007/978-3-642-74539-3_2
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