Immunological and Enzymatic Approaches of the Orientation of the Membrane Bound ADP/ATP Carrier

  • G. Brandolin
  • F. Boulay
  • P. Dalbon
  • M. Block
  • I. Gauche
  • P. V. Vignais

Abstract

The difficulties encountered in the crystallization of membrane proteins in a form suitable for X-ray analysis have stimulated the development of algorithms to predict, from primary amino acid sequences, the locations of transmembrane spans based upon assessment of local hydrophobicity. The proposed models for the folding of polypeptide chains of a variety of integral proteins predict membrane-spanning α-helices connected by hydrophilic, looplike segments. To test the validity of such models, much interest has been devoted to various probes that are important tools in studying the spatial orientation of membrane-spanning polypeptide chains (for review see Ovchinnikov, 1987).

Keywords

Crystallization Iodine Adenosine Arginine Cytosol 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Aquila H, Misra D, Eulitz M, Klingenberg M (1982) Complete amino acid sequence of the ADP/ATP carrier from beef heart mitochondria. Hoppe-Seyler’s Z Physil Chem 363; 345–349CrossRefGoogle Scholar
  2. Bogner W, Aquila H, Klingenberg M (1982) Surface labeling of membrane-bound ADP/ATP carrier by pyridoxal phosphate. FEBS Lett 146:259–261CrossRefGoogle Scholar
  3. Bogner W, Aquila H, Klingenberg M (1986) The transmembrane arrangement of ADP/ATP carrier as elucidated by the lysine reagent pyridoxal 5-phosphate. Eur J Biochem 165:611–620CrossRefGoogle Scholar
  4. Boulay F, Lauquin GJM, Tsugita A, Vignais PV (1983) Photolabeling approach to the study of the topography of the atractyloside binding site in mitochondrial adenosine 5’-diphosphate/adenosine 5’-triphosphate carrier protein. Biochemistry 22:477–484PubMedCrossRefGoogle Scholar
  5. Boulay F, Vignais PV (1984) Localization of the N-ethylmaleimide reactive cysteine in the beef heart mitochondria ADP/ATP carrier protein. Biochemistry 23:4807–4812PubMedCrossRefGoogle Scholar
  6. Boulay F, Lauquin GJM, Vignais PV (1986) Localization of immunoreactive regions in the beef heart adenine nucleotide carrier using rabbit antisera against the carboxy-atractyloside-liganded and the sodium dodecyl sulfate denaturated carrier forms. Biochemistry 25:7567–7571PubMedCrossRefGoogle Scholar
  7. Brdiczka D, Schumacher D (1976) Iodination of peripheral mitochondrial membrane proteins in correlation to the functional state of the ADP/ATP carrier. Biochem Biophys Res Commun 73:823–832PubMedCrossRefGoogle Scholar
  8. Buchanan BB, Eiermann W, Riccio P, Aquila H, Klingenberg M (1976) Antibody evidence for different conformational states of ADP/ATP translocator protein isolated from mitochondria. Proc Natl Acad Sci USA 73:2280–2284PubMedCrossRefGoogle Scholar
  9. Carrasco N, Herzlinger D, Mitchell R, Dechiara S, Danho W, Gabriel TF, Kaback HR (1984) Intramolecular dislocation of the COOH terminus of the lac carrier protein in reconstituted proteoliposomes. Proc Natl Acad Sci USA 81:4672–4676PubMedCrossRefGoogle Scholar
  10. Chavez LG, Sheraga HA (1979) Location of the antigenic determinants of bovine pancreatic ribonuclease. Biochemistry 18:4386–4395PubMedCrossRefGoogle Scholar
  11. Dalbon P, Brandolin G, Boulay F, Hoppe J, Vignais PV (1988) Mapping of the nucleotide-binding sites in the ADP/ATP carrier of beef heart mitochondria by photolabeling with 2-azido[α-32P]adenosine diphosphate. Biochemistry 27:5141–5149PubMedCrossRefGoogle Scholar
  12. Davies A, Meeran K, Cairns MT, Baldwin SA (1987) Peptide-specific antibodies as probes of the orientation of the glucose transporter in the human erythrocyte membrane. J Biol Chem 262:9347–9352PubMedGoogle Scholar
  13. Kimura K, Masson J, Khotzana HG (1982) Immunological probes for bacteriorhodopsin. Identification of three distinct antigenic sites on the cytoplasmic surface. J Biol Chem 257:2859–2867PubMedGoogle Scholar
  14. Klingenberg M (1985) The ADP/ATP carrier in mitochondrial membranes. In: Martonosi AN (ed) The Enzymes of Biological Membranes: Membrane Transport, vol 4. John Wiley, New York, pp 511–553Google Scholar
  15. Ovchinnikov YA (1987) Probing the folding of membrane proteins. Trends in Biol Sci 12:434–438CrossRefGoogle Scholar
  16. Runswick MJ, Powell SJ, Nyren P, Walker JE (1987) Sequence of the bovine mitochondrial phosphate carrier protein: structural relationship to ADP/ATP translocase and tne brown fat mitochondria uncoupling protein. EMBO J 6:1367–1373PubMedGoogle Scholar
  17. Saraste M, Walker JE (1982) Internal sequence repeats and the path of polypeptide in mitochondrial ADP/ATP translocase. FEBS Lett 144:250–254PubMedCrossRefGoogle Scholar
  18. Sekler R, Wright JK, Overath P (1983) Peptide-specific antibody locates the COOH terminus of the lactose carrier of Escherichia coli on the cytoplasmic side of the plasma membrane. J Biol Chem 258:10817–10820Google Scholar
  19. Thornton JM, Sibanda BL (1983) Amino and carboxy-terminal regions in globular proteins. J Mol Biol 167:443–460PubMedCrossRefGoogle Scholar
  20. Torok K, Joshi S (1985) Formation of an intramolecular disulfide bond in the mitochondrial adenine nucleotide translocase. FEBS Lett 182:340–344PubMedCrossRefGoogle Scholar
  21. Vignais PV, Block MR, Boulay F, Brandolin G, Lauquin GJM (1985) Molecular aspects of structure-function relationships in mitochondrial adenine nucleotide carrier. In: Bengha G (ed) Structure and Properties of Cell Membranes, vol 2. CRC Press, Boca Raton, pp 139–179Google Scholar
  22. Young EF, Ralston E, Blake J, Ramachandran J, Hall ZW, Strend RM (1985) Topological mapping of acetylcholine segments and a cytoplasmic COOH-terminal peptide. Proc Natl Acad Sci USA 82:626–630PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • G. Brandolin
    • 1
  • F. Boulay
    • 1
  • P. Dalbon
    • 1
  • M. Block
    • 1
  • I. Gauche
    • 1
  • P. V. Vignais
    • 1
  1. 1.DRF/LBio/BiochimieCentre d’Etudes Nucléaires, 85XGrenoble cedexFrance

Personalised recommendations