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Production of Hybridomas

  • Chapter
Monoclonal Antibodies

Part of the book series: Springer Laboratory ((SLM))

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Abstract

To be suitable for fusion, myeloma and other tumor cells must fulfil the following four conditions:

  1. 1.

    They may no longer synthesize intact antibody or immunoglobulin light or heavy chains themselves.

  2. 2.

    They must possess an enzyme defect, to allow their selective elimination after the fusion.

  3. 3.

    They should have good fusion properties, so that the number of hybridomas produced is as large as possible.

  4. 4.

    Their molecular properties should induce a high rate of MAB synthesis in the hybridoma.

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Authors
  1. D. Baron
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  2. J. H. Peters
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Editors and Affiliations

  1. Abteilung für Immunologie, Universität Göttingen, Kreuzbergring 57, W-3400, Göttingen, Germany

    Johann Hinrich Peters

  2. Forschungszentrum Tutzing, Boehringer Mannheim GmbH, Bahnhofstraße 9-15, W-8132, Tutzing, Germany

    Horst Baumgarten

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Baron, D. et al. (1992). Production of Hybridomas. In: Peters, J.H., Baumgarten, H. (eds) Monoclonal Antibodies. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74532-4_6

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