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Taking Blood and Isolating Cells

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Monoclonal Antibodies

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Abstract

It is possible to take up to 0.2 ml on repeated occasions from a narcotized living mouse (about 20 g), or approximately 0.7 ml of venous blood from the plexus retroorbitalis (more exactly: “plexus retrobulbaris”) on a single occasion (see a, below).

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Further Reading

  • Adegke AJH, Cohen J (1886) A better method for terminal bleeding of mice. Lab Anim 20:70–72

    Article  Google Scholar 

  • Baker HJ, Lindsey JR, Weisbroth SH (1980) The laboratory rat, vol 2. Research applications. Academic Press, New York

    Google Scholar 

  • Behrens H (1979) Lehrbuch der Schlafkrankheiten. Paul Parey, Berlin

    Google Scholar 

  • Foster HL, Small JD, Fox JG (1983) The mouse in the biomedicall research, vol 3. Academic Press, New York

    Google Scholar 

  • Fox JG, Cohen BJ, Loew FM (1984) Laboratory animal medicine. Academic Press, New York

    Google Scholar 

  • Jung S (1962) Grundlagen für die Zucht und Haltung der wichtigsten Versuchstiere. Gustav Fischer, Stuttgart

    Google Scholar 

Further Reading

  • Weir DM (ed) (1986) Handbook of experimental immunology in four volumes, vol 4. Applications of immunological methods in biomedical sciences. Blackwell, Oxford

    Google Scholar 

  • Glick JL (1980) Fundamentals of human lymphoid cell culture. Marcel Dekker, New York

    Google Scholar 

  • Hume DA, Weidemann MC (1980) Mitogenic lymphocyte transformation. Elsevier, Amsterdam

    Google Scholar 

  • Ling NR, Kay JE (1975) Lymphocyte stimulation. Elsevier, Amsterdam, pp 9–67

    Google Scholar 

  • Peters JH (1975) Preparation of large quantities of pure bovine lymphocytes and a monolayer technique for lymphocyte cultivation. Methods Cell Biol 9:1–11

    Article  PubMed  CAS  Google Scholar 

Reference

  • Bojum A (1968) Separation of leucocytes from blood and bone marrow. Scand J Clin Lab Invest 21:77–85

    Article  Google Scholar 

References

  • Barald KF (1987) Purification of antigen-specific B cells by adherence to whole-cell antigens. In: Pretlow TG, Pretlow TP (ed) Cell separation. Methods and selected applications. Academics Press, San Diego, pp 89–102

    Google Scholar 

  • Basch RS, Berman JW, Lakow E (1983) Cell separation using positive immunoselective techniques. J Immunol Methods 56:269–280

    Article  PubMed  CAS  Google Scholar 

  • Erkman L, Soldati G, James RW, Kato AC (1987) Partial purification of lymphoblasts after in vitro immunization increases the yield in Ig-producing hybridomas. J Immunol Methods 98:43–52

    Article  PubMed  CAS  Google Scholar 

  • Klausner A (1984) Hopkin Lab improves hybridoma production. Bio/Technology Sept: 743–744

    Google Scholar 

  • Kranz DM, Billing PA, Herron JN, Voss EW (1980) Modified hybridoma methodology: antigen-directed chemically mediated. Immunol Commun 9:639–651

    PubMed  CAS  Google Scholar 

  • Larsson PH, Hed J, Johansson SGO, Persson U, Wahlström M (1989) Improved cell depletion in a panning technique using covalent binding of immunoglobulins to surface modified polystyrene dishes. J Immunol Methods 116:293–298

    Article  PubMed  CAS  Google Scholar 

  • Mason DW, Penhale WJ, Sedgwick JD (1987) Preparation of lymphocytes subpopulations. In: Klaus GGB (ed) Lymphocytes. A practical approach. IRL Press, Oxford, pp 35–54

    Google Scholar 

  • Mirza IH, Wilkin TJ, Cantarini M, Moore K (1987) A comparison of spleen and lymph node cells as fusion partners for the raising of monoclonal antibodies after different routes of immunisation. J Immunol Methods 105:235–243

    Article  PubMed  CAS  Google Scholar 

  • Reason D, Carminati J, Kimura J, Henry C (1987) Directed fusion in hybridoma production. J Immunol Methods 99:253–257

    Article  PubMed  CAS  Google Scholar 

  • Stähli C, Staehelin Th, Miggiano V (1983) Spleen cell analysis and optimal immunization for high-frequency production of specific hybridoma. Methods Enzymol 92:27–36

    Google Scholar 

  • Wojchowski DM, Sytkowski AJ (1986) Hybridoma production by simplified avidin-mediated electrofusion. J Immunol Methods 90:173–177

    Article  PubMed  CAS  Google Scholar 

Further Reading

  • Fazekas de St Groth S, Scheidegger D (1980) Production of monoclonal antibodies: strategy and tactics. J Immunol Methods 35:1–21

    Article  Google Scholar 

  • Fox PC, Berenstein EH, Siraganian RP (1980) Enhancing the frequency of antigen-specific hybridomas. Eur J Immunol 11:431–434

    Article  Google Scholar 

  • Hlinak A, Jahn S, Grunow R, Mehl M, Heider G, Baehr R von (1987) Optimierungsversuche zu Klonierung von Maus-Maus und Mensch-Maus-Hybridomen unter Verwendung verschiedener Feederzelltypen. Mh Vet Med 42:801–804

    Google Scholar 

  • Kennet RH, McKearn TJ, Bechtol KB (1980) (eds) Monoclonal antibodies. Hybridomas: a new dimension in biological analyses. Plenum, New York

    Google Scholar 

  • Melchers F, Potter M, Warner N (eds) (1979) Lymphocyte hybridomas. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Sugasawara RJ, Cahoon BE, Karu AE (1985) The influence of murine macrophage-conditioned medium on cloning efficiency, antibody synthesis, and growth rate of hybridomas. J Immunol Methods 79/2:263–275

    Article  PubMed  CAS  Google Scholar 

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© 1992 Springer-Verlag Berlin Heidelberg

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Wiggenhauser, A., Peters, J.H., Baumgarten, H., Borgya, A. (1992). Taking Blood and Isolating Cells. In: Peters, J.H., Baumgarten, H. (eds) Monoclonal Antibodies. Springer Laboratory. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74532-4_4

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  • DOI: https://doi.org/10.1007/978-3-642-74532-4_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74534-8

  • Online ISBN: 978-3-642-74532-4

  • eBook Packages: Springer Book Archive

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