Abstract
Preparations of isolated vesicles represent an ideal system for the study of transport processes associated with native membranes. With this approach, it is possible to characterize single transport systems without the complications associated with examining transport in the intact cell. Since the vesicles are not associated with the metabolic machinery of the cell, solute transport can be studied in the absence of metabolic transformation or compartmentation and the energy supply to drive transport (e.g., ATP) can be added exogenously.
Keywords
- Membrane Vesicle
- Proton Transport
- Plasma Membrane Vesicle
- Proton Electrochemical Gradient
- Plasma Membrane ATPase
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
Abbreviations
- BTP:
-
bis-tris propane
- CCCP:
-
carbonylcyanide m-chlorophenylhydrazone
- DES:
-
diethylstilbestrol
- DCCD:
-
N,N′-dicyclohexylcarbodiimide
- EPR:
-
electron paramagnetic resonance
- PMSF:
-
phenylmethylsulfonyl fluoride
References
Bennett AB, Spanswick RM (1983) Optical measurement of ΔpH and ΔΨ in corn root membrane vesicles: kinetic analysis of Ch effects on a proton translocating ATPase. J Membr Biol 71: 95–107
Bennett AB, O’Neill SD, Spanswick RM (1984) H+-ATPase from storage tissue of Beta vulgaris I. Identification and characterization of an anion sensitive H+-ATPase. Plant Physiol 74: 538–544
Blumwald E (1987) Tonoplast vesicles as a tool in the study of ion transport at the plant vacuole. Physiol Plant 69: 731–734
Blumwald E, Poole RJ (1985a) Nitrate storage and retrieval in Beta vulgaris L.: effects of nitrate and chloride on proton gradients in tonoplast vesicles. Proc Natl Acad Sci USA 83: 3683–3687
Blumwald E, Poole RJ (1985b) Na+ /H+ antiport in isolated tonoplast vesicles from storage tissues of Beta vulgaris. Plant Physiol 78: 163–167
Blumwald E, Poole RJ (1986) Kinetics of Ca2+ /H+ antiport in isolated tonoplast vesicles from storage tissue of Beta vulgaris L. Plant Physiol 80: 727–731
Briars SA, Kessler F, Evans DE (1989) The calmodulin-stimulated ATPase of maize coleoptiles is a 140,000 Mr polypeptide. Planta 176: 283–285
Briskin DP (1987) Plasma membrane H+-transporting ATPase: role in potassium ion transport? Physiol Plant 68: 159–163
Briskin DP, Poole RJ (1983) Characterization of a K+-stimulated adenosine triphosphatase associated with the plasma membrane of red beet. Plant Physiol 71: 350–355
Briskin DP, Thornley WR, Wyse RE (1985) Membrane transport in isolated vesicles from sugarbeet taproot. II. Evidence for a sucrose/H+-antiport. Plant Physiol 78: 871–875
Briskin DP, Leonard RT, Hodges TK (1987) Isolation of plasma membrane: membrane markers and general principles. Methods Enzymol 148: 542–558
Buckhout TJ (1983) ATP-dependent Ca2+ -transport in endoplasmic reticulum isolated from roots of Lepidium sativum L. Planta 159: 84–90
Buckhout TJ (1984) Characterization of Ca2+-transport in purified endoplasmic reticulum membranes from Lepidium sativum L. roots. Plant Physiol 76: 962–967
Bush DR, Langston-Unkefer PJ (1988) Amino acid transport into membrane vesicles isolated from zucchini: evidence for a H+/amino acid symport in the plasmalemma. Plant Physiol 88: 487–490
Bush DR, Sze H (1986) Calcium transport in tonoplast and endoplasmic reticulum vesicles isolated from cultured carrot cells. Plant Physiol 80: 549–555
Canut H, Brightman AO, Boudet AM, Morre DJ (1987) Determination of sidedness of plasma membrane and tonoplast vesicles isolated from plant stems. In: Leaver C, Sze H (eds) Plant membranes: structure, function and biogenesis. Alan R Liss, pp 141–159
Churchill KA, Sze H (1983) Anion sensitive, H+-pumping ATPase in membrane vesicles from oat roots. Plant Physiol 71: 610–617
Churchill KA, Holloway B, Sze H (1983) Separation of two types of electrogenic H+-pumping ATPases from oat roots. Plant Physiol 73: 921–928
Deamer DW, Prince RC, Crofts AR (1972) The response of fluorescent amines to pH gradients across liposome membranes. Biochim Biophys Acta 274: 323–335
De Michelis MI, Spanswick RM (1986) H+-pumping driven by the vanadate-ATPase in membrane vesicles from corn roots. Plant Physiol 81: 542–547
Dieter P, Marme D (1980) Calmodulin activation of plant microsomal Ca2+ uptake. Proc Natl Acad Sci USA 77: 7311–7314
Dieter P, Marme D (1981) A calmodulin-dependent, microsomal ATPase from corn (Zea mays L.). FEBS Lett 125: 245–248
Dupont FM, Bennett AB, Spanswick RM (1982) Localization of a proton-translocating ATPase on sucrose gradients. Plant Physiol 70: 1115–1119
Dupont FM, Tanaka CK, Hurkman WJ (1988) Separation and immunological characterization of membrane fractions from barley roots. Plant Physiol 86: 717–724
Federico R, Giartosio CE (1983) A trans-plasma membrane electron transport system in maize roots. Plant Physiol 73: 182–184
Furst P, Solioz M (1986) The vanadate-sensitive ATPase of Streptococcus faecalis pumps potassium in a reconstituted system. J Biol Chem 261: 4302–4308
Giannini JL, Briskin DP (1987) Proton transport in plasma membrane and tonoplast vesicles from red beet (Beta vulgaris L.) storage tissue. A comparative study of ion effects on ΔpH and ΔΨ. Plant Physiol 84: 613–618
Giannini JL, Briskin DP (1988) Pyridine nucleotide oxidation by a plasma membrane fraction from red beet (Beta vulgaris L.) storage tissue. Arch Biochem Biophys 260: 653–660
Giannini JL, Gildensoph LH, Briskin DP (1987a) Selective production of sealed plasma membrane vesicles from red beet (Beta vulgaris L.) storage tissue. Arch Biochem Biophys 254: 621–630
Giannini JL, Gildensoph LH, Ruiz-Cristin J, Briskin DP (1987b) Isolation and characterization of sealed plasma membrane vesicles from red beet (Beta vulgaris L.) storage tissue. Plant Physiol Suppl 83: 55
Giannini JL, Gildensoph LH, Reynolds-Niesman I, Briskin DP (1988a) Calcium transport in sealed vesicles from red beet (Beta vulgaris L.) storage tissue. I. Characterization of a Ca2+-pumping ATPase associated with the endoplasmic reticulum. Plant Physiol 85: 1129–1136
Giannini JL, Ruiz-Cristin J, Briskin DP (1988b) Calcium transport in sealed vesicles from red beet (Beta vulgaris L.) storage tissue. II. Characterization of 45Ca2+ uptake into plasma membrane vesicles. Plant Physiol 85: 1137–1142
Giannini JL, Holt JS, Briskin DP (1988c) Isolation of sealed plasma membrane vesicles from Phytophthora megasperma f. sp. glycinea. I. Characterization of proton pumping and ATPase activity. Arch Biochem Biophys 265: 337–345
Gunn RB (1980) Co- and counter transport mechanisms in cell membranes. Annu Rev Physiol 42: 249–259
Hager A, Helme M (1981) Properties of an ATP fueled, CI-dependent proton pump localized in membranes of microsomal vesicles from maize coleoptiles. Z Naturforsch 36c: 927–937
Hager A, Frenzel R, Laible D (1980) ATP-dependent proton transport into vesicles of microsomal membranes of Zea mays coleoptiles. Z Naturforsch 35c: 783–793
Hepler PK, Wayne RO (1986) Calcium and plant development. Annu Rev Plant Physiol 36: 397–439
Itano T, Penniston JT (1985) Ca2+-pumping ATPase of plasma membranes. In: Cheung A (ed) Calmodulin antagonists and cellular physiology. Academic Press, New York, pp 335–345
Kaback HR (1974a) Transport in isolated bacterial vesicles. Methods Enzymol 31: 698–709
Kaback HR (1974b) Transport studies in bacterial membrane vesicles. Science 186: 882–892
Kaback HR (1983) The lac carrier protein in Escherichia coli. J Membr Biol 76: 95–112
Larsson C, Kjellbom P, Widell S, Lundborg T (1984) Sidedness of plant plasma membrane vesicles purified by partitioning in aqueous two-phase systems. FEBS Lett 171: 271–276
Larsson C, Widell S, Kjellbom P (1987) Preparation of high-purity plasma membranes. Methods Enzymol 148: 558–568
Larsson C, Widell S, Sommarin M (1988) Inside-out plant plasma membrane vesicles of high purity obtained by aqueous two-phase partitioning. FEBS Lett 229: 289–292
Lee HC, Forte JG (1978) A study of H+ transport in gastric microsomal vesicles using fluorescent probes. Biochim Biophys Acta 508: 339–359
Leonard RT (1984) Membrane associated ATPases and nutrient absorption by roots. In: Tinker PB, Lauchli A (eds) Advances in plant nutrition, Vol I. Praeger Scientific, New York, pp 209–240
Lew RR, Spanswick RM (1984) Proton-pumping activities of soybean (Glycine max L.) root microsomes: localization and sensitivity to nitrate and vanadate. Plant Sci Lett 36: 187–193
Lew RR, Bushunow N, Spanswick RM (1985) ATP-dependent proton-pumping activities of zucchini fruit microsomes. A study of tonoplast and plasma membrane activities. Biochim Biophys Acta 821: 341–347
Marre MT, Moroni A, Albergoni FG, Marre E (1988) Plasmalemma redox activity and H+ extrusion. I. Activation of the H+-pump by ferricyanide-induced potential depolarization and cytoplasm acidification. Plant Physiol 87: 25–29
Melhorn RJ, Packer L (1984) Bioenergetic studies of cells with spin probes. Ann NY Acad Sci 414: 180–189
Melhorn RJ, Candu P, Packer L (1982) Measurements of volumes and electrochemical gradients with spin probes in membrane vesicles. Method Enzymol 88: 752–761
Mercier J, Poole RJ (1980) Electrogenic pump activity in red beet: its relation to ATP levels and cation influx. J Membr Biol 55: 165–174
Mettler I J, Leonard RT (1979) Ion transport in isolated protoplasts from tobacco suspension cells. I. General characteristics. Plant Physiol 63: 183–190
Mitchell PM (1976) Vectorial chemistry and the molecular mechanism of chemiosmotic coupling: power transmission by proticity. Biochem Soc Trans 4: 399–430
Mitchell PM (1985) The correlation of chemical and osmotic forces in biochemistry. J Biochem 97: 1–18
Møller I, Lin W (1986) Membrane-bound NAD(P)H dehydrogenases in higher plant cells. Annu Rev Plant Physiol 37: 309–334
Møller IM, Lundborg T, Bérczi A (1984) The negative surface charge density of plasmalemma vesicles from wheat and oat roots. FEBS Lett 167: 181–185
O’Neill SD, Spanswick RM (1984) Solubilization and reconstitution of a vanadate-sensitive H+-ATPase from the plasma membrane of Beta vulgaris. J Membr Biol 79: 245–256
Perlin DS, Spanswick RM (1982) Isolation and assay of corn root membrane vesicles with reduced proton permeability. Biochim Biophys Acta 690: 178–186
Poole RJ (1982) Electrogenic transport at the plasma membrane of plant cells. In: Martinosi AN (ed) Membranes and transport, vol 2. Plenum Publishing, New York, pp 651–655
Poole RJ, Briskin DP, Kratky Z, Johnstone RM (1984) Density gradient localization of plasma membrane and tonoplast from storage tissue of growing and dormant red beet: characterization of proton transport and ATPase in tonoplast vesicles. Plant Physiol 74: 549–556
Poole RJ, Melhorn RJ, Packer L (1985) A study of transport in tonoplast vesicles using spin-labelled probes. In: Marin B (ed) Biochemistry and function of vacuolar adenosine triphosphatase in fungi and plants. Springer, Berlin, Heidelberg New York Tokyo, pp 114–118
Poovaiah BW, Reddy ASN (1987) Calcium messenger system in plants. CRC Crit Rev Plant Sci 6: 47–103
Pope A J, Leigh RA (1988) Dissipation of pH gradients in tonoplast vesicles and liposomes by mixtures of acridine orange and anions. Implications of the use of acridine orange as a ΔpH probe. Plant Physiol 86: 1315–1322
Ramos S, Kaback HR (1977) The electrochemical proton gradient in Escherichia coli membrane vesicles. Biochemistry 16: 848–854
Rasi-Caldogno F, Pugliarello MC, De Michelis MI (1985) Electrogenic transport of protons driven by the plasma membrane ATPase in membrane vesicles from radish. Biochemical characterization. Plant Physiol 77: 200–205
Rea PA, Poole RJ (1985) Proton-translocating inorganic pyrophosphatase in red beet (Beta vulgaris L.) tonoplast vesicles. Plant Physiol 77: 46–52
Robinson C, Larsson C, Buckhout TJ (1988) Identification of a calmodulin-stimulated (Ca2+ + Mg2+)-ATPase in a plasma membrane fraction isolated from maize (Zea mays) leaves. Physiol Plant 72: 177–184
Rodriguez-Navarro A, Blatt MR, Slayman CL (1986) A potassium-proton symport in Neurospora crassa. J Gen Physiol 87: 649–674
Rubinstein B, Stern AI (1986) Relationship of transplasmalemma redox activity to proton and solute transport by roots of Zea mays. Plant Physiol 80: 805–811
Rubinstein B, Stern AI, Stout RG (1984) Redox activity at the surface of oat root cells. Plant Physiol 76: 386–391
Ruiz-Cristin J, Briskin DP (1988) Nitrate transport in plasma membrane vesicles from red beet (Beta vulgaris L.) storage tissue. Plant Physiol Suppl 86: 79
Schumaker KS, Sze H (1985) A Ca2+/H+ antiport system driven by the proton electrochemical gradient of a tonoplast H+ -ATPase from oat roots. Plant Physiol 79: 1111–1117
Sijmons PC, Lanfermeijer FC, De Boer AH, Prins HBA, Bienfait HF (1984) Depolarization of cell membrane potential during trans-plasma membrane electron transfer to extracellular electron acceptors in iron-deficient roots of Phaseolus vulgaris L. Plant Physiol 76: 943–946
Singh SP, Kesav B VS, Briskin DP (1987) Reconstitution and rapid partial purification of the red beet plasma membrane ATPase. Physiol Plant 69: 617–626
Steck TL (1974) Preparation of impermeable inside-out and right-side-out vesicles from erythrocyte membranes. Methods Membr Biol 2: 245–281
Stein WD (1986) Transport and diffusion across cell membranes. Academic Press, Orlando, FL
Sze H (1980) Nigericin-stimulated ATPase activity in microsomal vesicles of tobacco callus. Proc Natl Acad Sci USA 77: 5904–5908
Sze H (1982) Characterization of nigericin-stimulated ATPase from sealed vesicles of tobacco callus. Plant Physiol 70: 495–505
Sze H (1983) H+-pumping ATPase in membrane vesicles of tobacco callus: sensitivity to vanadate and K+. Biochim Biophys Acta 732: 586–594
Sze H (1985) H+-translocating ATPases: advances using membrane vesicles. Annu Rev Plant Physiol 36: 175–208
Sze H, Hodges TK (1976) Characterization of passive ion transport in plasma membrane of oat roots. Plant Physiol 58: 304–308
Turner RJ (1983) Quantitative studies of co-transport systems: models and vesicles. J Membr Biol 76: 1–15
Van Thieman B, Postma PW (1973) Coupling between energy conservation and active transport of serine in E. coli. Biochim Biophys Acta 323: 429–440
Vara F, Serrano R (1982) Partial purification and properties of the proton-translocating ATPase of plant plasma membranes. J Biol Chem 252: 5334–5336
Waggoner AS (1979) Dye indicators of membrane potential. Annu Rev Biophys Bioeng 8: 47–68
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Kluwer Academic Publishers
About this chapter
Cite this chapter
Briskin, D.P. (1989). Transport in Plasma Membrane Vesicles — Approaches and Perspectives. In: Larsson, C., Møller, I.M. (eds) The Plant Plasma Membrane. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74522-5_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-74522-5_7
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-74524-9
Online ISBN: 978-3-642-74522-5
eBook Packages: Springer Book Archive