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A Critical Evaluation of Markers Used in Plasma Membrane Purification

  • Chapter
The Plant Plasma Membrane

Abstract

A membrane marker is any property that can be used to identify a specific membrane. In the ideal case, the marker used is not only confined to a single membrane, but is also uniformly distributed in the membrane, thus constituting an absolute marker for that membrane (Quail 1979). Meaning, that wherever the marker is found the membrane is present in direct proportion to the marker. Very few, if any, of the markers currently in use fulfill the above requirements. Rather, most markers have one primary location and one, or several, secondary locations. Furthermore, the marker may be non-uniformly distributed in the membrane due to membrane lateral heterogeneity, or may be absent in certain cell types. Two markers which are close to ideal are cytochrome c oxidase of the mitochondrial inner membrane and chlorophyll for chloroplast thylakoids, although lateral heterogeneity is a striking feature of the latter membrane (review Andersson and Anderson 1980). By contrast, antimycin A-insensitive NAD(P)H-cytochrome c reductase, the most commonly used marker for the endoplasmic reticulum, is in fact found in several membranes (review, Møller and Lin 1986) and is far from an ideal marker.

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Abbreviations

BSA:

bovine serum albumin

BTP:

bis-Tris propane

CCD:

countercurrent distribution

DCCD:

N,N′-dicyclohexylcarbodiimide

DTT:

dithiothreitol

GS I:

glucan synthase I or 1,4-β-glucan synthase

GS II:

glucan synthase II or 1,3-β-glucan synthase

HEPES:

N-(2-hydroxyethyl)piperazine-N′-2-ethanesulfonic acid

MES:

2-(N-morpholino)ethanesulfonic acid

MOPS:

3-(N-morpholino)propanesulfonic acid

NAA:

naphthylacetic acid

NPA:

naphthylphthalamic acid

PMSF:

phenylmethylsulfonyl fluoride

PTA:

phosphotungstic acid

PVPP:

polyvinylpolypyrrolidone

SDS:

sodium dodecyl sulfate

STA:

silicotungstic acid

Tris:

tris(hydroxymethyl)aminomethane

UDPG:

UDP-glucose

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Authors and Affiliations

  1. Department of Plant Physiology, University of Lund, P.O. Box 7007, S-220 07, Lund, Sweden

    S. Widell

  2. Department of Plant Biochemistry, University of Lund, P.O. Box 7007, S-220 07, Lund, Sweden

    C. Larsson

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  1. S. Widell
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Editors and Affiliations

  1. Department of Plant Biochemistry, University of Lund, Box 7007, S-220 07, Lund, Sweden

    Christer Larsson

  2. Department of Plant Physiology, University of Lund, Box 7007, S-220 07, Lund, Sweden

    Ian M. Møller

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Widell, S., Larsson, C. (1990). A Critical Evaluation of Markers Used in Plasma Membrane Purification. In: Larsson, C., Møller, I.M. (eds) The Plant Plasma Membrane. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74522-5_2

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  • DOI: https://doi.org/10.1007/978-3-642-74522-5_2

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