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Electron Probe Microanalysis pp 59–72Cite as

The Subcellular Accumulation of Toxic Heavy Metals: Qualitative and Quantitative X-Ray Microanalysis

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Part of the book series: Springer Series in Biophysics ((BIOPHYSICS,volume 4))

Abstract

Several qualitative microprobe studies have been focused on the distribution of essential and non-essential metals between the various types of metal-rich invertebrate spherites (Brown, 1982; Morgan, 1984; Morgan and Winters, 1987). The conclusion drawn from these observations is that, in general and despite the competitive tendancy extant in multimetal and multi-ligand situations in vivo, the metals are preferentially bound to cellular ligands according to their equilibrium constants for metal-ligand complexes (Nieboer and Richardson, 1980): Class A (‘hard acid’) metals such as Ca, Mg, Mn, Sr and Pb show a preference for oxygen donating ligands according to the sequence O>N>S; Class B (’soft acid’) metals such as Cd, Cu, Hg conform to the ligand sequence S>N>O (Simkiss, 1981; Simkiss and Taylor, 1981; Simkiss et al., 1982; Morgan and Winters, 1987).

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References

  • Bailey NTJ (1981) Statistical methods in biology, 2nd edn. Hodder and Stoughton, London.

    Google Scholar 

  • Brown BE (1982). The form and function of metal-containing ‘granules’ in invertebrate tissues. Biol Rev 57: 621–667.

    Article  CAS  Google Scholar 

  • Hagler HK Buja LM (1984) New techniques for the preparation of thin freeze dried cryosections for X-ray microanalysis. In: Revel JP Barnard T Haggis GH (eds) Science of biological specimen preparation Scanning Electron Microscopy Inc., AMF O’Hare, Chicago, p 161.

    Google Scholar 

  • Jones HB Morgan AJ Lovell DP Bellamy D. (1986) Statistical analyses of multielement electronprobe data correlated with morphological changes in stored human erythrocytes. Micron Microscopica Acta 17: 307–317.

    Article  Google Scholar 

  • Jones HB Morgan AJ Lovell DP Bellamy D (1988). Identification of chemically-unique individual cells of an erythrocyte population during storage in Acid Citrate Dextrose: analysis of electronprobe data by analysis of variance. Proc Roy Microsc Soc 23/4: 212.

    Google Scholar 

  • Lupton JB Saubermann AJ (1986) A new and simple method for preparation of thin aminoplastic standards for X-ray microanalysis. J. Microsc., 144: RP3–4.

    Article  PubMed  CAS  Google Scholar 

  • Morgan AJ (1984) The localization of heavy metals in the tissues of terrestrial invertebrates by electron microprobe X-ray analysis. Scanning Electron Microsc 1984/IV: 1847–1865.

    Google Scholar 

  • Morgan AJ (1985) X-ray microanaiysis in electron microscopy for biologists. Oxford University Press/Royal Microscopical Society, Oxford.

    Google Scholar 

  • Morgan AJ Davies T Erasmus DA (1975) Analysis of droplets from isoatomic solutions as a means of calibrating a transmission electron analytical microscope (TEAM). J. Microsc 104: 271–280.

    Article  Google Scholar 

  • Morgan AJ Morris B (1982) The accumulation and intracellular compartmentation of cadmium, lead, zinc and calcium in two earthworms species (Dendrobaena rubida and Lumbricus rubellus) living in highly contaminated soil. Histochemistry 75: 269–285.

    Article  PubMed  CAS  Google Scholar 

  • Morgan AJ Winters C (1987). The contribution of electron probe X-ray microanaiysis (EPXMA) to pollution studies. Scanning Microscopy 1: 133–157.

    CAS  Google Scholar 

  • Morgan AJ Winters C (1988a) Standards for the quantification of light and heavy elements by the continuum normalisation method: thin aminoplastic sections and sprayed microdroplets. Proc Roy Microsc Soc 23/4: 207–208.

    Google Scholar 

  • Morgan AJ Winters C (1988b). Practical notes on the production of thin aminoplastic standards for quantitative X-ray microanaiysis.

    Google Scholar 

  • Morgan JE Morgan AJ (1988a) Earthworms as biological monitors of cadmium, copper, lead and zinc in metalliferous soils. Environ Pollut 54: 123–138.

    Article  PubMed  CAS  Google Scholar 

  • Morgan JE Morgan AJ (1988b) Zinc sequestration by earthworms (Annelida: Oligochaeta) chloragocytes. An in vivo investigation using fully quantitative electron probe X-ray micro-analysis. Histochemistry (in press).

    Google Scholar 

  • Morgan JE Morgan AJ (1988c submitted). The effect of lead incorporation on the elemental composition of earthworm (Annelida: Oligochaeta) chloragosome granules.

    Google Scholar 

  • Morgan JE Morgan AJ (1988d) Calcium-lead interactions involving earthworms. Part 2: The effect of accumulated lead on endogenous calcium in Lumbricus rubellus. Environ Poilut 55: (in press).

    Google Scholar 

  • Morgan JE Norey CG Morgan AJ and Kay J (1988) A comparison of the cadmium-binding proteins isolated from the posterior alimentary canal of the earthworms Dendrodrilus rubidus and Lumbricus rubeilus. Comp Biochem Physiol (in press).

    Google Scholar 

  • Nieboer E Richardson DHS (1980). The replacement of the non-descript term ‘heavy metal’ by a biologically and chemically significant classification of metal ions. Environ Pollut (Ser B) 1: 3–26.

    Article  CAS  Google Scholar 

  • Roomans GM (1979) Standards for X-ray microanalysis of biological specimens. Scan Electron Microsc/1980/II: 309–320.

    Google Scholar 

  • Roomans GM (1988) Quantitative X-ray microanalysis of biological specimens. J Electron Microsc Tech 9: 19–43.

    Article  PubMed  CAS  Google Scholar 

  • Roos N Barnard T (1984) Aminoplastic standards of quantitative X-ray microanalysis of thin sections of plastic-embedded biological material. Ultramicroscopy 15: 277–286.

    Article  PubMed  CAS  Google Scholar 

  • Roos N Morgan AJ (1985). Aminoplastic standard for electron probe X-ray microanalysis (EPXMA) of ultrathin frozen-dried cryosections. J Microsc 140: RP3–4.

    Article  Google Scholar 

  • Simkiss K (1976) Intracellular and extracellular routes in biomineralisation Symp Soc Exp Biol 30: 423–444.

    CAS  Google Scholar 

  • Simkiss K (1981) Cellular discrimination processes in metal accumulating cells J exp Biol 94: 317–347.

    CAS  Google Scholar 

  • Simkiss K Jenkins KGA McLellan J Wheeler E (1982) Methods of metal incorporation into intracellular granules. Experientia 38: 333–335.

    Article  CAS  Google Scholar 

  • Simkiss K Taylor M. (1981) Cellular mechanisms of metal ion detoxification and some new indices of pollution. Aquatic Toxicol 1: 279–290.

    Article  CAS  Google Scholar 

  • Somlyo AP Bond M Somlyo AV (1985) Calcium content of mitochondria and endoplasmic reticulum in liver frozen rapidly in vivo. Nature 314: 622–625.

    Article  PubMed  CAS  Google Scholar 

  • Winters C Morgan AJ (1988) Quantitative electron probe X-ray microanalysis of lead-sequestering organelles in earthworms: technical appraisal of air-dried smears and freeze dried cryosections. Scanning Microscopy 2: 947–958.

    CAS  Google Scholar 

  • Zierold K (1988) X-ray microanalysis of freeze-dried and frozen-hydrated cryosections. J. Electron Microsc. Tech 9: 65–82.

    CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. School of Pure and Applied Biology, University of Wales College of Cardiff, P.O. Box 915, Cardiff, CF1 3TL, UK

    A. J. Morgan, J. E. Morgan & C. Winters

  2. E.M.L.B. University of Oslo, P.O. Box 1062, Blindern, Oslo, Norway

    N. Roos

Authors
  1. A. J. Morgan
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  2. N. Roos
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  3. J. E. Morgan
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  4. C. Winters
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Editor information

Editors and Affiliations

  1. Max-Planck-Institut für Systemphysiologie, Rheinlanddamm 201, 4600, Dortmund 1, Germany

    Karl Zierold

  2. Department of Pathology, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, TX, 75235-9072, USA

    Herbert K. Hagler Ph.D. (Associate Professor of Pathology) (Associate Professor of Pathology)

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© 1989 Springer-Verlag Berlin Heidelberg

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Morgan, A.J., Roos, N., Morgan, J.E., Winters, C. (1989). The Subcellular Accumulation of Toxic Heavy Metals: Qualitative and Quantitative X-Ray Microanalysis. In: Zierold, K., Hagler, H.K. (eds) Electron Probe Microanalysis. Springer Series in Biophysics, vol 4. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74477-8_5

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  • DOI: https://doi.org/10.1007/978-3-642-74477-8_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74479-2

  • Online ISBN: 978-3-642-74477-8

  • eBook Packages: Springer Book Archive

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