Resin Based Standards for Biological Energy Dispersive X-Ray and Electron Energy Loss Microanalysis

  • D. C. Joy
  • C. S. Joy
  • D. A. Armstrong
Part of the Springer Series in Biophysics book series (BIOPHYSICS, volume 4)

Abstract

Elemental standards, or calibration specimens, can perform several important functions in biological microanalysis. Firstly for the quantification of EDS data from ultrathin biological section using Halls’ method (1971) they provide a means of determining the weight factor for a given element. Secondly they provide an objective and reproducible way of testing the performance of the analytical system in such areas as its absolute sensitivity and its freedom from system induced artifacts (Williams and Steel 1987). Thirdly they provide a library of peaks and edges for use in the iterative reconvolution quantification techniques developed for application to cases where severe peak or edge overlaps occur in a biological system (Leapman and Swyt 1987), for use in statistical tests to determine the validity of an edge in a noisy spectrum (Joy 1982), and for use as comparison spectra for such cases as those where the valence state of an element in an unknown is to be determined. In order to meet these needs in our own laboratory we have been developing a family of calibration specimens containing elements of biological interest at concentrations from 10 to in excess of 100 mmol/Kg.

Keywords

Zinc Epoxy Brittle Chlorine Expense 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • D. C. Joy
    • 1
  • C. S. Joy
    • 1
  • D. A. Armstrong
    • 1
  1. 1.EM FacilityUniversity of TennesseeKnoxvilleUSA

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