Skip to main content
SpringerLink
Log in
Book cover

Biological Characterization of Bone Tumors pp 115–152Cite as

Flow Cytometric DNA Analysis of Bone Tumors

  • Chapter

Part of the book series: Current Topics in Pathology ((CT PATHOLOGY,volume 80))

Abstract

It is the foremost objective of every tumor Classification to help to determine prognosis. The definition of morphological categories is the generally accepted principle of all current classifications. In bone tumor pathology, the spectrum of morphological criteria has been enlarged by roentgenological features; nevertheless, their Classification is often more difficult than that of carcinomatous neoplasms (Schajowicz etal. 1972; Schajowicz 1981). This is due not only to the great morphological heterogeneity of bone tumors but also to the close topographical relationship between tumor tissue and the often very similar mesenchymal changes of inflammatory, reactive, or strongly proliferative nature. A major feature is the presence of multinucleated, histiocytic giant cells in bone tumors. Though varying in intensity, the occurrence of giant cells is a typical trait. Differential diagnostic problems are encountered, especially in the morphological distinction of purely reactive alterations from well-differentiated but potentially malignant neoplasms (e.g., parosteal osteosarcoma), and of benign from malignant tumorous lesions (e.g., benign and malignant giant cell tumors).

This is a preview of subscription content, log in via an institution.

Chapter
USD   29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD   84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD   109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Learn about institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Ahuja SC, Villacin AB, Smith J, Bullough PG, Huvos AG (1977) Juxtacortical (parosteal) osteogenic sarcoma. Histological grading and prognosis. J Bone Joint Surg 59: 632–647

    PubMed  CAS  Google Scholar 

  • Alho A, Connor JF, Mankin HJ, Schiller AL, Crawford JC (1983) Assessment of malignancy of cartilage tumors using flow cytometry. A preliminary report. J Bone Joint Surg 65: 779–785

    PubMed  CAS  Google Scholar 

  • Allan CJ, Soule EH (1971) Osteogenic sarcoma of the somatic soft tissues. Clinicopathologic study of 26 cases and review of the literature. Cancer 27: 1121

    Article  PubMed  CAS  Google Scholar 

  • Astorino RN, Tesluk H (1985) Dedifferentiated chondrosarcoma with a rhabdomyosarcomatous component. Hum Pathol 16: 318–320

    Article  PubMed  CAS  Google Scholar 

  • Atkin NB, Mattison G, Baker MC (1966) A comparison of the DNA content and chromosome number of fifty human tumors. Br J Cancer 20: 87–101

    Article  PubMed  CAS  Google Scholar 

  • Baisch H, Göhde W, Linden WA (1975) Analysis of PCP data to determine the fraction of cells in the various phases of cell cycle. Radiat Environ Biophys 12: 31–39

    Article  PubMed  CAS  Google Scholar 

  • Barlogie B, Hittelman W, Spitzer G, Trujilla JM, Hart JS, Smallwood L, Drewinko B (1977) Correlation of DNA distribution abnormalities with cytogenetic findings in human adult leukemia and lymphoma. Cancer Res 37: 4400–4407

    PubMed  CAS  Google Scholar 

  • Barlogie B, Latreille J, Freireich EJ, Fu CT, Mellard D, Meistrich M, Andreef M (1980) Characterisation of hematologic malignancies by flow cytometry. Blood Cells 6: 719–744

    PubMed  CAS  Google Scholar 

  • Barlogie B, Raber MN, Schumann J et al. (1983) Flow cytometry in clinical cancer research. Cancer Res 43: 3982–3997

    PubMed  CAS  Google Scholar 

  • Bertoni F, Unni KK, McLeod RA, Dahlin DC (1985) Osteosarcoma resembling osteoblastoma. Cancer 55: 416–426

    Article  PubMed  CAS  Google Scholar 

  • Bösing T, Roessner A, Hiddemann W, Mellin W, Grundmann E (1987) Cytostatic effects in osteosarcomas as detected by flow cytometric DNA analysis after preoperative chemotherapy according to the COSS 80/82 protocol.

    Google Scholar 

  • Braylan RC, Fowlkes BJ, Jaffe ES, Sanders SK, Berard CW, Herman CJ (1978) Cell volumes and DNA distributions of normal and neoplastic human lymphoid cells. Cancer 41: 201–209

    Article  PubMed  CAS  Google Scholar 

  • Brittinger W (Kiel Lymphoma Study Group) (1981) Principles and present status of a prospective multicenter study on the clinical relevance of the Kiel Classification. Blut 43: 155–166

    Article  Google Scholar 

  • Büchner T, Bloomfield CD, Hiddemann W, Hossfeld DK, Schumann J (1985) Tumor aneuploidy. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Costa A, Mazzini G, Del Bino G, Silvestrini R (1981) DNA content and kinetic characteristics of non-Hodgkin’s lymphoma: determined by flow cytometry and autoradiography. Cytometry 2: 185–188

    Article  PubMed  CAS  Google Scholar 

  • Cuvelier CA, Roels HJ (1979) Cytophotometric studies of the nuclear DNA content in cartilaginous tumors. Cancer 4: 1363–1374

    Article  Google Scholar 

  • Dahlin DC, Unni KK (1986) Bone tumors. General aspects and data on 8,542 cases. Charles C. Thomas, Springfield, pp 269–321

    Google Scholar 

  • Dahlin DC, Cupps RE, Johnson EW Jr (1970) Giant cell tumor: a study of 195 cases. Cancer 25: 1061–1070

    Article  PubMed  CAS  Google Scholar 

  • Darzynkiewicz Z, Traganos F, Andreeff M, Sharpless T, Melamed MR (1979) Different sensitivity of chromatin to acid denaturation in quiescent and cycling cells as revealed by flow cytometry. J Histochem Cytochem 27: 478

    Article  PubMed  CAS  Google Scholar 

  • Dean PN, Jett JH (1974) Mathematical analysis of DNA distributions derived from flow microfluorometry. J Cell Biol 60: 523–527

    Article  PubMed  CAS  Google Scholar 

  • Diamond LW, Braylan RC (1980) Flow analysis of DNA content and cell size in non-Hodgkin’s lymphoma. Cancer Res 40: 703–712

    PubMed  CAS  Google Scholar 

  • Diamond LW, Nathwani BN, Rappaport H (1982) Flow cytometry in the diagnosis and Classification of malignant lymphoma and leukemia. Cancer 50: 1122–1135

    Article  PubMed  CAS  Google Scholar 

  • Dittrich W, Göhde W (1969) Impulsfluorometrie bei Einzelzellen in Suspension. Z Naturforsch 24: 360

    CAS  Google Scholar 

  • Dorfman HD (1972) Malignant transformation of benign bone lesions. Seventh National Cancer Conference Proceedings 901–913

    Google Scholar 

  • Dorfman HD, Weiss SW (1984) Borderline osteoblastic tumors: problems in the differential diagnosis of aggressive osteoblastoma and low-grade osteosarcoma. Semin Diagn Pathol 1: 215–234

    PubMed  CAS  Google Scholar 

  • Dumont J, Mazabraud A (1979) Primary lymphoma of bone: histological review of 75 cases according to the new Classification of non-Hodgkin’s lymphomas. Biomedicine 31: 271–275

    PubMed  CAS  Google Scholar 

  • Edeiken J, Hodes P (1973) Roentgen diagnosis of diseases of bone, 2nd edn. Williams amp Wilkins, Baltimore, pp 998–999

    Google Scholar 

  • Enzinger FM (1977) Recent developments in the Classification of soft tissue sarcomas. In: Management of primary bone and soft tissue sarcomas. Year Book Medical Publishers, Chicago

    Google Scholar 

  • Enzinger FM, Weiss SW (1983) Soft tissue tumors. C. V. Mosby, St. Louis, pp 166–198

    Google Scholar 

  • Erlandson RA, Tandler B, Liebermann HP, Higinbotham NL (1968) Ultrastructure of human chordoma. Cancer Res 28: 2115–2125

    PubMed  CAS  Google Scholar 

  • Feldman F, Lattes R (1977) Primary malignant fibrous histiocytoma (fibrous Xanthoma) of bone. Skeletal Radiol 1: 145–160

    Article  Google Scholar 

  • Feldman F, Norman D (1972) Intra- and extraosseous malignant histioma (malignant fibrous Xanthoma). Radiology 104: 497–508

    PubMed  CAS  Google Scholar 

  • Franke HD (1972) Die Anwendung strahlensensibilisierender Substanzen in der Strahlentherapie. Strahlentherapie 143: 296

    PubMed  CAS  Google Scholar 

  • Friedmann I, Harrison DFN, Bird ES (1962) The fine structure chordoma with particular reference to the physaliferous cell. J Clin Pathol 15: 116–125

    Article  PubMed  CAS  Google Scholar 

  • Frindel E, Tubiana M (1971) Radiobiology and the cell cycle. In: Baserga R (ed) The Cell cycle and cancer. Marcel Dekker, New York, pp 391–447

    Google Scholar 

  • Gee VR, Pugh DG (1958) Giant cell tumour of bone. Radiology 70: 33–41

    PubMed  CAS  Google Scholar 

  • Gelfant S (1977) A new concept of tissue and tumor cell proliferation. Cancer Res 27: 3845

    Google Scholar 

  • Goldenberg RP, Campbell CJ, Bonfiglio M (1970) Giant cell tumor of bone. An analysis of 218 cases. J Bone Joint Surg 52: 619–664

    PubMed  CAS  Google Scholar 

  • Gresen AA, Dahlin DC, Peterson CFA, Payne WSP (1973) Benign giant cell tumor of bone metastasizing to the lung. Ann Thorac Surg 16: 531–535

    Article  PubMed  CAS  Google Scholar 

  • Grundmann E, Mellin W, Roessner A, Hiddemann W, Vollmer E, Edel G, Lingg G (1985) Zur Klassifikation und Prognose der Osteosarkome im Erfahrungsgut des Knochengeschwulstregisters Westfalen unter Einbezug proliferationskinetischer Parameter. Dtsch Ges Osteol 221–226

    Google Scholar 

  • Hagemeister FB, Raber M, Barlogie B, Maddox A, Cabanillas F (1981) Flow cytometry analysis in lymphoma: effect of abnormal DNA content. Proc Am Assoc Cancer Res 22: 42

    Google Scholar 

  • Hammarberg C, Slezak P, Tribukait B (1984) Early detection of malignancy in ulcerative Colitis. A flow cytometric study. Cancer 53: 291–295

    Article  PubMed  CAS  Google Scholar 

  • Hedley DW, Friedländer ML, Taylor JW, Rugg CA, Musgrove EA (1983) Method for analysis of cellular DNA content of paraffin-embedded pathological material using flow cytometry. J Histochem Cytochem 31: 1333–1335

    Article  PubMed  CAS  Google Scholar 

  • Hedley DW, Friedländer ML, Taylor IW (1985) Application of DNA flow cytometry to paraffin-embedded archival material for the study of aneuploidy and its clinical significance. Cytometry 6: 327–333

    Article  PubMed  CAS  Google Scholar 

  • Heliö H, Karaharju E, Nordling S (1985) Flow cytometric determination of DNA content in malignant and benign bone tumors. Cytometry 6: 165–171

    Article  PubMed  Google Scholar 

  • Hiddemann W, Roessner A, Wörmann B etal. (1987) Tumor heterogeneity in osteosarcoma as identified by flow cytometry. Cancer 59: 324–328

    Article  PubMed  CAS  Google Scholar 

  • Huvos AG (1976) Primary malignant fibrous histiocytoma of bone. Clinicopathologic study of 18 patients. NY State J Med 76: 552–559

    CAS  Google Scholar 

  • Huvos AG (1979) Bone tumors: diagnosis, treatment and prognosis. Saunders, Philadelphia, pp 94–106

    Google Scholar 

  • Inada O, Yumoto T, Furuse K, Tanaka T (1976) Ultrastructural features of malignant fibrous histiocytoma of bone. Acta Pathol Jpn 24: 491–501

    Google Scholar 

  • Jaffe HL, Lichtenstein L, Portis RB (1940) Giant cell tumor of bone. Its pathologic appearance, grading, supposed variants and treatment. Arch Pathol 30: 993–1031

    Google Scholar 

  • Johnson WW, Cobrun TP, Pratt CB, Smith JW, Kumar APM, Dahlin DC (1978) Ultrastructure of MFH arising in the acromion. Hum Pathol 9: 199–209

    Article  PubMed  CAS  Google Scholar 

  • Jürgens H (1981) Ewing-Sarkom bei Kindern und Jugendlichen: Planung einer kooperativen Thera¬piestudie der Gesellschaft für Pädiatrische Onkologie (CESS 81). Klin Pädiat 193: 254–257

    Article  Google Scholar 

  • Jürgens H, Göbel V, Michaelis J et al. (1985) Die kooperative Ewing-Sarkom Studie CESS 81 der GPO-Analyse nach 4 Jahren. Klin Pädiatr 1978: 225–232

    Article  Google Scholar 

  • Katenkamp D, Stiller D (1981) Malignant fibrous histiocytoma of bone. Light microscopic and electron microscopic examination offour cases. Virchows Arch 391: 323–335

    CAS  Google Scholar 

  • Kreicbergs A, Zetterberg A, Söderberg G (1980) The prognostic significance of nuclear DNA content in human chondrosarcoma. Analy Quant Cytol Histol 2: 272–279

    CAS  Google Scholar 

  • Kreicbergs A, Boquist L, Borssen B, Larsson S-E (1982) Prognostic factors in chondrosarcoma. A comparative study of cellular DNA content and clinicopathologic features. Cancer 50: 57–583

    Article  Google Scholar 

  • Kreicbergs A, Silvferswärd C, Tribukait B (1984) Flow DNA analysis of primary bone tumors. Relationship between cellular DNA content and histopathologic Classification. Cancer 53: 129–136

    Article  PubMed  CAS  Google Scholar 

  • Krishan A (1975) Rapid flow cytophotometric analysis of mammalian cell cycle by propidium iodide staining. J Cell Biol 66: 18

    Google Scholar 

  • Krishan A (1976) Effect of ädriamycine on the cell cycle traverse and kinetics of cultured lymphoblasts. Cancer Res 36: 143

    PubMed  CAS  Google Scholar 

  • Krishan A, Palka KD, Frei E (1976) Cell cycle Synchronisation of human lymphoid cells in vitro by 2,3-dihydro-1H-imidazo(l,2-b) pyrazole. Cancer Res 38: 138

    Google Scholar 

  • Krug H (1977) Der Wert der Impulszytophotometrie in der klinischen Onkologie. Acta Histochem 19: 167–172

    Google Scholar 

  • Llombart-Bosch A, Blache R, Peydro-Olaya A (1982) Roundcell sarcomas of bone and their differential diagnosis (with particular emphasis on Ewing’s sarcoma and reticulosarcoma). Pathol Annu 17 (2): 113–145

    PubMed  Google Scholar 

  • Macartney JC, Camplejohn RS (1986) DNA flow cytometry of histological material from dysplastic lesions of human gastric mucosa. J Pathol 150: 113–118

    Article  PubMed  CAS  Google Scholar 

  • Mankin HJ, Connor JF, Schiller AL, Perlmutter N, Alho A, McGuire M (1985) Grading of bone tumors by analysis of nuclear DNA content using flow cytometry. J Bone Joint Surg 67: 404–413

    PubMed  CAS  Google Scholar 

  • Martinez-Tello FJ, Navas-Palacios JJ, Calvo-Asensio M, Loizaga Iriondo JM (1981) Malignant fibrous histiocytoma of bone. A clinico-pathological and electron-microscopical study. Pathol Res Pract 173: 141–158

    Article  PubMed  CAS  Google Scholar 

  • Mellin W (1986) Möglichkeiten und Grenzen zytophotometrischer Meßverfahren bei der Untersuchung von Tumoren und Präkanzerosen. MD-GBK: Mitteilungsdienst — Gesellschaft zur Bekämpfung der Krebskrankheiten NRW 49: 46–49

    Google Scholar 

  • Mellin W, Grundmann E (1989) Flow cytometric analysis of stemline heterogeneity. Pathol Res Pract 184: 6–10

    Article  Google Scholar 

  • Mellin W, Roessner A, Wörmann B, Vollmer E, Hiddemann W, Grundmann E (1984) Evaluation of dignity in metastasizing giant cell tumors of bone by flow cytometric DNA analysis. Verh Dtsch Ges Pathol 68: 220–223

    Google Scholar 

  • Mellin W, Wörmann B, Roessner A, Hiddemann W, Immenkamp M, Grundmann E (1985) Biological characterisation of human bone tumors. VII. Detection of malignancy in a giant cell tumor of bone by flow cytometric DNA analysis. Pathol Res Pract 180: 619–625

    Article  PubMed  CAS  Google Scholar 

  • Mellin W, Roessner A, Ritter J, Sapp H, Grundmann G (1985) Zur durchflußzytophotometrischen DNA-Analyse des Ewing-Sarkoms. Verh Dtsch Ges Pathol 69: 606

    Google Scholar 

  • Mellin W, Davaris P, Jütte G, Heidi G, Krieg V, Grundmann E (1986a) Comparative flow cytometrical analysis of DNA and CEA in gastric carcinomas. Verh Dtsch Ges Pathol 70: 234–237

    CAS  Google Scholar 

  • Mellin W, Roessner A, Immenkamp M, Boeddinghaus D, Grundmann E (1986b) Klassifikation und Differentialdiagnose der juxtakortikalen Osteosarkome. Pathologe 7: 94–100

    PubMed  CAS  Google Scholar 

  • Mellin W, Roessner A, Grundmann E (1986) Flow cytometric DNA analysis of malignant fibrous histiocytoma of bone. Deutscher Krebskongress 1986. J Cancer Res Clin Oncol 111: 149

    Article  Google Scholar 

  • Mellin W, Roessner A, Pill C, Edel G, Grundmann E (1987) Durchflußzytophotometrische DNA-Analyse an Osteoblastomen. Verh Dtsch Ges Path (in press)

    Google Scholar 

  • Mellin W, Roessner A, Schöneich U, Vollmer E, Grundmann E (1987) Durchflußzytophotometrische DNA-Analyse an Chordomen. Verh Dtsch Ges Pathol (in press)

    Google Scholar 

  • Mikuz G, Mydla F (1974) Elektronenmikroskopische und zytophotometrische Untersuchungen des Chordoms. Verh Dtsch Ges Pathol 58: 447–453

    PubMed  CAS  Google Scholar 

  • Mikuz G, Mydla F, Gütter W (1977) Chordoma: ultrastructural, biochemical and cytophotometric findings. Beitr Pathol 161: 150–165

    PubMed  CAS  Google Scholar 

  • Mnaymneh WA, Dudley HR, Mnaymneh LG (1964) Giant cell tumor of bone. An analysis and follow-up study of the 41 cases observed at the Massachusetts General Hospital between 1925 and 1960. J Bone Joint Surg 46: 63–79

    PubMed  CAS  Google Scholar 

  • Murad TM, Murthy MSN (1970) Ultrastructure of chordoma. Cancer 25: 1204–1215

    Article  PubMed  CAS  Google Scholar 

  • Niezabitowski A, Edel G, Roessner A, Timm C, Wuisman P, Grundmann E (1987) Rhabdomyosarcomatous component in dedifferentiated chondrosarcoma. Pathol Res Pract 182: 275–279

    Article  PubMed  CAS  Google Scholar 

  • Ozzello L, Stout AP, Murray MR (1963) Cultural characteristics of malignant histiocytomas and fibrous Xanthomas. Cancer 16: 331

    Article  PubMed  CAS  Google Scholar 

  • Pena CE, Horvat BL, Fisher ER (1970) The ultrastructure ofa chordoma. Am J Clin Pathol 53: 544–551

    PubMed  CAS  Google Scholar 

  • Pfitzer P (1985) The karyological basis of atypia. Pathol Res Pract 180: 675–681

    Article  PubMed  CAS  Google Scholar 

  • Rao U, Cheng A, Didolkar MS (1978) Extraosseous osteogenic sarcoma. Cancer 41: 1488

    Article  PubMed  CAS  Google Scholar 

  • Rock MG, Pritchard DJ, Unni KK (1984) Metastases from histologically benign giant cell tumor of bone. J Bone Joint Surg 66: 263–273

    Google Scholar 

  • Roessner A (1984) Zur Zyto- und Histogenese der malignen und semimalignen Knochentumoren. Gustav-Fischer, Stuttgart New York

    Google Scholar 

  • Roessner A, Hobik HP, Grundmann E (1979) Malignant fibrous histiocytoma of bone and osteosarcoma. A comparative light and electron microscopic study. Pathol Res Pract 164: 385–401

    Article  PubMed  CAS  Google Scholar 

  • Rosen G, Caparros B, Nirenberg A et al. (1981) Ewing’s sarcoma: ten-year experience with adjuvant chemotherapy. Cancer 47: 2204–2213

    Article  PubMed  CAS  Google Scholar 

  • Salzer-Kuntschik M, Brand G, Delling G (1983) Bestimmung des morphologischen Regressionsgrades nach Chemotherapie bei malignen Knochentumoren. Pathologe 4: 135–141

    PubMed  CAS  Google Scholar 

  • Salzer-Kuntschik M, Delling G, Beron G, Sigmund R (1983) Morphological grades of regression in osteosarcoma after polychemotherapy — study COSS 80. J Cancer Res Clin Oncol 106: 2124

    Article  Google Scholar 

  • Sandberg AA (1980) The chromosomes in human cancer and leukemia. Elsevier/North Holland, New York

    Google Scholar 

  • Sandritter W, Grimm H (1977) DNA in non-Hodgkin’s lymphoma — a cytophotometric study. Beitr Pathol 160: 213–230

    PubMed  CAS  Google Scholar 

  • Schajowicz F (1981) Tumors and tumorlike lesions of bone and joints. Springer, Berlin Heidelberg New York

    Google Scholar 

  • Schajowicz F, Lemos C (1976) Malignant osteoblastoma. J Bone Joint Surg 588: 202–211

    Google Scholar 

  • Schajowicz F, Ackermann LV, Sissons HA (1972) Histological typing of bone tumours. Int histol classif of tumours No. 6, WHO, Geneva

    Google Scholar 

  • Schutte B, Reynders MMJ, Bosman FT, Blijham GH (1985) Flow cytometric determination of DNA ploidy level in nuclei isolated from paraffin-embedded tissue. Cytometry 6: 26–30

    Article  PubMed  CAS  Google Scholar 

  • Shackney SE, Skramstadt KS (1979) A dynamic interpretation of multiparameter studies in human lymphomas. Am J Clin Pathol 72: 756–764

    PubMed  CAS  Google Scholar 

  • Shackney SE, Skramstadt KS, Cunningham RE, Dugas DJ, Lincoln TL, Lukes RJ (1980) Dual Parameter flow cytometry studies in human lymphomas. J Clin Invest 66: 1281–1294

    Article  PubMed  CAS  Google Scholar 

  • Shackney SE, Levine AM, Fisher RI et al. (1984) The biology of tumor growth in the non-Hodgkin’s lymphomas. A dual parameter flow cytometry study of 220 cases. J Clin Invest 73: 1201–1214

    Article  PubMed  CAS  Google Scholar 

  • Silvestrini R, Piazza R, Riccardi A, Rilke F (1977) Correlation of cell kinetic fmdings with morphology of non-Hodgkin’s malignant lymphomas. J Natl Cancer Inst 58: 499–504

    PubMed  CAS  Google Scholar 

  • Sinclair W (1972) Cell cycle dependence of the lethal radiatic response in mammalian cells. Curr Top Radiation Res 7: 264

    Google Scholar 

  • Spanier SS (1977) Malignant fibrous histiocytoma of bone. Orthop Clin North Am 8: 947–961

    Google Scholar 

  • Spjut H, Luse SA (1964) Chordoma: an electron microscopic study. Cancer 17: 643–656

    Article  PubMed  CAS  Google Scholar 

  • Stenzinger W, Suter I, Schumann J (1984) DNA aneuploidy in congenital melanocytic nevi: suggestive evidence for premalignant changes. J Invest Dermatol 82: 569–572

    Article  PubMed  CAS  Google Scholar 

  • Tobey RA, Crissmann HA (1972) Use of flow microfluorometry in detailed analysis of effects of chemical agents on cell cycle progression. Cancer Res 32: 2726–2732

    PubMed  CAS  Google Scholar 

  • Unni KK, Dahlin DC, Beabout JW (1976) Periosteal osteogenic sarcoma. Cancer 37: 2476–2485

    Article  PubMed  CAS  Google Scholar 

  • Unni KK, Dahlin DC, Beabout JW, Ivins JC (1976) Parosteal osteogenic sarcoma. Cancer 37: 2466–2475

    Article  PubMed  CAS  Google Scholar 

  • Unni KK, Dahlin DC, McLeod RA, Pritchard DJ (1977) Intraosseous well-differentiated osteosarcoma. Cancer 40: 1337–1347

    Article  PubMed  CAS  Google Scholar 

  • Vassallo J, Roessner A, Vollmer E, Grundmann E (1987) Malignant lymphomas with primary bone manifestation. Pathol Res Pract 182: 381–384

    PubMed  CAS  Google Scholar 

  • Vassallo J, Mellin W, Pill C, Roessner A, Grundmann E (1987) Flow cytometric DNA analysis of malignant lymphomas with primary bone manifestation. J Cancer Res Clin Oncol 113: 249–252

    Article  PubMed  CAS  Google Scholar 

  • Vollmer E, Roessner A, Gerdes J, Mellin W, Stein H, Chong-Schachel S, Grundmann E (1986) Improved grading of bone tumors with the monoclonal antibody Ki-67. J Cancer Res Clin Oncol 112: 281–282

    Article  PubMed  CAS  Google Scholar 

  • Williams AH, Schwinn CP, Parker JW (1976) The ultrastructure of osteosarcoma — a review of 20 cases. Cancer 37: 1293

    Article  PubMed  CAS  Google Scholar 

  • Willnow U (1982) Autoradiographic in vitro study of the cell kinetics of osteogenic sarcomas and Ewing’s sarcomas in children. Arch Geschwulstforsch 52: 9–16

    PubMed  CAS  Google Scholar 

  • Winkler K, Beron G, Kotz R et al. (1983) Adjuvant chemotherapy in osteosarcoma. Effects of Cislatinum, BCD and flbroblast interferon in sequential combination with DH/MTX and adriamycin. Preliminary results of the COSS-80 study. J Cancer Res Clin Oncol 106: 1–7

    Article  PubMed  Google Scholar 

  • Winkler K, Beron G, Kotz R et al. (1984) Neoadjuvant chemotherapy for osteogenic sarcoma: results of a cooperative German/Austrian study. J Clin Oncol 2: 6

    Google Scholar 

  • Wold LE, Unni KK, Beabout JW, Pritchard DJ (1984) Highgrade surface osteosarcomas. Am J Surg Pathol 8: 181–186

    Article  PubMed  CAS  Google Scholar 

Download references

Authors
  1. W. Mellin
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Dierschauer
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. W. Hiddemann
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. A. Roessner
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. G. Edel
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. P. Wuisman
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. A. Härle
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. E. Grundmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. Gerhard-Domagk-Institut für Pathologie der Universität, Domagkstraße 17, D-4400, Münster, Germany

    A. Roessner (Prof. Dr.) (Prof. Dr.)

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Mellin, W. et al. (1989). Flow Cytometric DNA Analysis of Bone Tumors. In: Roessner, A. (eds) Biological Characterization of Bone Tumors. Current Topics in Pathology, vol 80. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74462-4_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-74462-4_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-74464-8

  • Online ISBN: 978-3-642-74462-4

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation