Protoplast Culture and Transformation Studies on Rice
Rice (Oryza sativa L.), which is one of the representative crops of Gramineae plants, has been cultivated for the past 10000 years. Rice grain contains a high amount of protein and amino acids and is highly nutritious. The plant has the highest yield per unit area of all the small grains and has the advantage in that even a small area can feed a large population. At present, it is the most important world crop, together with wheat, and supplies about half of the world population with the principal food in the temperate and the tropical zones. Most other staple cereals — barley, corn, rye, oat — belong to the Gramineae. Many monocotyledonous grasses such as forage plants also belong to the same family. Therefore, the studies of tissue culture and genetic manipulation in Gramineae plants are of great importance for the agricultural industry. The cultivation and regeneration of protoplasts is important as a prerequisite for the use of various technologies for breeding such as gene transfer and somatic cell fusion.
KeywordsCrown Gall Protoplast Isolation Protoplast Culture Rice Cell Transformation Study
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