Advertisement

Uptake and Integration of Exogenous DNA in Plants

  • P. F. Lurquin
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)

Abstract

Representative organisms of the Monera, fungi, animals, and plants can be modified genetically via uptake of nonviral foreign DNA molecules. This phenomenon is now considered rather commonplace and the articles published on the subject are so numerous that it would be unfair to many authors to cite only a few. In the case of the Eukaryotae, this added genetic information has been shown to be expressed phenotypically in progeny cells and whole organisms and was thus stably maintained following mitosis, meiosis, fertilization, and morphogenesis.

Keywords

Chimeric Gene Crown Gall Plant Protoplast Tobacco Protoplast Direct Gene Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. An G (1985) High efficiency transformation of cultured tobacco cells. Plant Physiol 79, 568–570PubMedCrossRefGoogle Scholar
  2. Barker H, Harrison D (1982) Infection of potato mesophyll protoplasts with five plant viruses. Plant Cell Rep 1: 247–249CrossRefGoogle Scholar
  3. Bevan MW, Flavell RB, Chilton M-D (1983) A chimaeric antibiotic resistance gene as a selectable marker for plant cell transformation. Nature 304: 184–187CrossRefGoogle Scholar
  4. Capecchi MR (1980) High efficiency transformation by direct microinjection of DNA into cultured mammalian cells. Cell 22: 479–488PubMedCrossRefGoogle Scholar
  5. Christen AA, Lurquin PF (1983) Infection of cowpea mesophyll protoplasts with cowpea chlorotic mottle virus (CCMV) RNA encapsulated in large liposomes. Plant Cell Rep 2:43–46Google Scholar
  6. Crossway A, Oakes JV, Irvine JM, Ward B, Knauf VC, Shewmaker CK (1986) Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts. Mol Gen Genet 202: 179–185CrossRefGoogle Scholar
  7. Cutler AJ, Shargool PD, Kurz WGW, Constabel F (1984) Liposomes as vehicles for transferring low molecular weight compounds into periwinkle protoplasts. J Plant Physiol 117: 29–40Google Scholar
  8. Davey MR, Freeman J, Draper J (1979) Transformation of higher plant protoplasts by isolated Agrobacterium plasmids. Abstr Fifth Int Protoplast Symp, July 9–14 Szeged, Hungary, p 137Google Scholar
  9. Davey MR, Cocking EC, Freeman J, Pearce N, Tudor I (1980) Transformation of Petunia protoplasts by isolated Agrobacterium plasmids. Plant Sci Lett 18: 307–313CrossRefGoogle Scholar
  10. de la Pena A, Lörz H, Schell J (1987) Transgenic rye plants obtained by injecting DNA into young floral tillers. Nature 325: 274–276CrossRefGoogle Scholar
  11. Deshayes A, Herrera-Estrella L, Caboche M (1985) Liposome mediated transformation of tobacco mesophyll protoplasts by an Escherichia coli plasmid. EMBO J 4: 2731–2737PubMedGoogle Scholar
  12. Draper J, Davey MR, Freeman JP, Cocking EC, Cox BJ (1982) Ti plasmid homologous sequences present in tissues from Agrobacterium plasmid-transformed Petunia protoplasts. Plant Cell Physiol 23: 451–458Google Scholar
  13. Ecker JR, Davis RW (1986) Inhibition of gene expression in plant cells by expression of antisense RNA. Proc Natl Acad Sci USA 83: 5372–5376PubMedCrossRefGoogle Scholar
  14. Faustmann O, Kern R, Sanger HL, Muhlbach H-P (1986) Potato spindle tuber viroid ( PSTV) RNA oligomers of (+) and (−) polarity are synthesized in potato protoplasts after liposome-mediated infection with PSTV. Virus Res 4: 213–227CrossRefGoogle Scholar
  15. Fernandez SM, Lurquin PF, Kado CI (1978) Incorporation and maintenance of recombinant-DNA plasmid vehicles pBR313 and pCR1 in plant protoplasts. FEBS Lett 87: 277–282PubMedCrossRefGoogle Scholar
  16. Fraley RT, Dellaporta SL, Papahadjopoulos D (1982) Liposome-mediated delivery of tobacco mosaic virus RNA into tobacco protoplasts: A sensitive assay for monitoring liposome-protoplast interactions. Proc Natl Acad Sci USA 79: 1859–1863PubMedCrossRefGoogle Scholar
  17. Fraley RT, Rogers SG, Horsch RB, Sanders PR, Flick JS, Adams SP, Bittner ML, Brand LA, Fink DL, Fry JS, Galluppi GR, Goldberg SB, Hoffmann NL, Woo SC (1983) Expression of bacterial genes in plant cells. Proc Natl Acad Sci USA 80: 4803–4807PubMedCrossRefGoogle Scholar
  18. Fromm ME, Taylor LP, Walbot V (1985) Expresssion of genes electroporated into monocot and dicot plant cells. Proc Natl Acad Sci USA 82: 5804–5828CrossRefGoogle Scholar
  19. Fromm ME, Taylor LP, Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature 319: 791–793PubMedCrossRefGoogle Scholar
  20. Fukunaga Y, Nagata T, Takebe I (1981) Liposome-mediated infection of plant protoplasts with tobacco mosaic virus RNA. Virology 113: 752–760PubMedCrossRefGoogle Scholar
  21. Gheysen G, Dhaese P, Van Montagu M, Schell J (1985) DNA flux across genetic barriers: The crown gall phenomenon. In: Hohn B, Dennis ES (eds) Genetic flux in plants. Springer, Berlin Heidelberg New York Tokyo, pp 11–47CrossRefGoogle Scholar
  22. Graham FL, Van der Eb AJ, Heijneker HL (1974) Size and location of the transforming regions in human adenovirus type 5 DNA. Nature 251: 687–691PubMedCrossRefGoogle Scholar
  23. Hain R, Stabel P, Czernilofsky AP, Steinbiss HH, Herrera-Estrella L, Schell J (1985) Uptake, integration, expression and genetic transmission of a selectable chimeric gene by plant protoplasts. Mol Gen Genet 199: 161–168CrossRefGoogle Scholar
  24. Herrera-Estrella L, Depicker A, Van Montagu M, Schell J (1983) Expression of chimeric genes transferred into plant cells using a Ti-plasmid-derived vector. Nature 303: 209–213CrossRefGoogle Scholar
  25. Hess D, Lorz H, Weissert E-V (1974) Uptake of bacterial DNA into swelling and germinating pollen grains of Petunia hybridia and Nicotiana glauca. Z Pflanzenphysiol 74: 52–63Google Scholar
  26. Hess D, Schneider G, Lorz H, Blaich G (1976) Investigations on the tumor induction in Nicotiana glauca by pollen transfer of DNA isolated from Nicotiana langsdorfii. Z Pflanzenphysiol 77: 247–254Google Scholar
  27. Hughes BG, White FG, Smith MA (1979) Fate of bacterial plasmid DNA during uptake by barley and tobacco protoplasts: II. Protection by poly-L-ornithine. Plant Sci Lett 14:303–310.CrossRefGoogle Scholar
  28. Kao KN, Michayluk MR (1974) A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115:355–367Google Scholar
  29. Kleinhofs A, Behki R (1977) Prospects for plant genome modification by nonconventional methods. Annu Rev Genet 11: 79–101PubMedCrossRefGoogle Scholar
  30. Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti-plasmid DNA. Nature 296: 72–74CrossRefGoogle Scholar
  31. Krens FA, Mans RMW, van Slogteren TMS, Hoge JHC, Wullems GJ, Schilperoort RA (1985) Structure and expression of DNA transferred to tobacco via transformation of protoplasts with Tiplasmid DNA: Co-transfer of T-DNA and non T-DNA sequences. Plant Mol Biol 5: 223–234CrossRefGoogle Scholar
  32. Langridge WHR, Li BJ, Szalay AA (1985) Electric field mediated stable transformation of carrot protoplasts with naked DNA. Plant Cell Rep 4: 355–359CrossRefGoogle Scholar
  33. Leber B, Hemleben V (1979) Uptake of homologous DNA into nuclei of seedlings and by isolated nuclei of a higher plant. Z Pflanzenphysiol 91: 305–316Google Scholar
  34. Ledoux L, Huart R (1969) Fate of exogenous bacterial deoxyribonucleic acids in barley seedlings. J Mol Biol 43: 243–262PubMedCrossRefGoogle Scholar
  35. Ledoux L, Huart R, Jacobs M (1974) DNA-mediated genetic correction of thiamineless Arabidopsis thaliana. Nature 249: 17–21PubMedCrossRefGoogle Scholar
  36. Lörz H, Baker B, Schell J (1985) Gene transfer to cereal cells mediated by protoplasts transformation. Mol Gen Genet 199: 178–182CrossRefGoogle Scholar
  37. Lurquin PF (1977) Integration versus degradation of exogenous DNA in plants: An open question. In: Cohn WE (ed) Progress in nucleic acid research and molecular biology, vol. 20. Academic Press, New York pp 161–207Google Scholar
  38. Lurquin PF (1979) Entrapment of plasmid DNA by liposomes and their interactions with plant protoplasts. Nucleic Acids Res 6: 3773–3784PubMedCrossRefGoogle Scholar
  39. Lurquin PF (1984) Entrapment of genetic material into liposomes and delivery to cells. In: Gregoriadis G (ed) Liposome technology. CRC Press, Boca Raton, pp 187–193Google Scholar
  40. Lurquin PF (1987) Foreign gene expression in plant cells. In: Cohn WE, Moldave K (eds) Progress in nucleic acid research and molecular biology. Academic Press, New York, Vol 34, pp 143–188Google Scholar
  41. Lurquin PF, Marton L (1980) DNA transfer experiments with plant protoplasts and bacterial plasmids. In: Farkas G, Dudits D, Ferenczy L (eds) Advances in protoplast research. Pergamon New York, pp 389–405Google Scholar
  42. Marton L, Wullems GJ, Molendijk L, Schilperoort RA (1979a) In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens. Nature 277:129–131Google Scholar
  43. Marton L, Wullems GJ, Lurquin PF, Molendijk L, Schilperoort RA (1979b) Crown gall transformation of tobacco protoplasts by Ti plasmid DNA from Agrobacterium tumefaciens. Abstr Fifth Int Protoplast Symp July 9–14 Szeged, Hungary, p 136Google Scholar
  44. Maule AJ, Boulton MI, Edmunds C, Wood KR (1980) Polyethylene glycol-mediated infection of cucumber protoplasts by cucumber mosaic virus and virus RNA. J Gen Virol 47: 199–203CrossRefGoogle Scholar
  45. Morikawa H, Iida A, Matsui C, Ikegami M, Yamada Y (1986) Gene transfer into intact plant cells by electroinjection through cell walls and membranes. Gene 41: 121–124PubMedCrossRefGoogle Scholar
  46. Muller A, Manzara T, Lurquin PF (1984) Crown gall transformation of tobacco callus cells by cocultivation with Agrobacterium tumefaciens. Biochem Biophys Res Commun 123: 458–462PubMedCrossRefGoogle Scholar
  47. Nagata T, Melchers G (1978) Surface charge of protoplasts and their significance in cell-cell interaction. Planta 142: 235–238CrossRefGoogle Scholar
  48. Nagata T, Okada K, Takebe I, Matsui C (1981) Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Mol Gen Genet 184: 161–165Google Scholar
  49. Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982) Gene transfer into mouse lyoman cells by electroporation in high electric fields. EMBO J 1: 841–845PubMedGoogle Scholar
  50. Ohta Y (1986) High-efficiency genetic transformation of maize by a mixture of pollen and exogenous DNA. Proc Natl Acad Sci USA 83: 715–719PubMedCrossRefGoogle Scholar
  51. Ow DW, Wood KV, DeLuca M, De Wet JR, Helinski DR, Howell SH (1986) Transient and stable expression of the firefly luciferase gene in plant cells and transgenic plants. Science 234: 856–859PubMedCrossRefGoogle Scholar
  52. Paszkowski J, Saul MW (1986) Direct gene transfer to plants. In: Weissbach H, Weissbach A (eds) Methods in enzymology, vol. 118. Academic Press, pp 668–675Google Scholar
  53. Paszkowski J, Shillito RD, Saul M, Mandak V, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3: 2717–2722PubMedGoogle Scholar
  54. Paszkowski J, Pisan B, Shillito RD, Hohn T, Hohn B, Potrykus I (1986) Genetic transformation of Brassica campestris var. rapa protoplasts with an engineered cauliflower mosaic virus genome. Plant Mol Biol 6: 303–312CrossRefGoogle Scholar
  55. Peerbolte R, Krens FA, Mans RMW, Floor M, Hoge GHC, Wullems GJ, Schilperoort RA (1985) Transformation of plant protoplasts with DNA: Cotransformation of non-selected calf thymus carrier DNA and meiotic segregation of transforming DNA sequences. Plant Mol Biol 5: 235–246CrossRefGoogle Scholar
  56. Pietrzak M, Shillito RD, Hohn T, Potrykus I (1986) Expression in plants of two bacterial antibiotic resistance genes after protoplast transformation with a new plant expression vector. Nucleic Acids Res 14: 5857–5866PubMedCrossRefGoogle Scholar
  57. Pollock K, Barfield DG, Robinson Si, Shields R (1985) Transformation of protoplast-derived cell colonies and suspension cultures by Agrobacterium tumefaciens. Plant Cell Rep 4: 202–205CrossRefGoogle Scholar
  58. Potrykus I, Saul MW, Petruska J, Paszkowski J, Shillito RD (1985a) Direct gene transfer to cells of a graminaceous monocot. Mol Gen Genet 199: 183–188CrossRefGoogle Scholar
  59. Potrykus I, Paszkowski J, Saul MW, Petruska J, Shillito RD (1985b) Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer. Mol Gen Genet 199: 169–177PubMedCrossRefGoogle Scholar
  60. Power JB, Davey MR, Freeman JP, Mulligan BJ, Cocking EC (1986) Fusion and transformation of plant protoplasts. In: Weissbach H, Weissbach A (eds) Methods in Enzymology, vol. 118. Academic Press, New York, pp 578–588Google Scholar
  61. Redei GP, Acedo G, Weingarten H, Kier LD (1976) Has DNA corrected genetically thiamineless mutants of Arabidopsis? In: Dudits D, Farkas GL, Maliga P (eds) Cell Genetics in Higher plants. Akademic Kiado, Budapest, pp 91–94Google Scholar
  62. Riggs CD, Bates GW (1986) Stable transformation of tobacco by electroporation: Evidence for plasmid concatenation. Proc Natl Acad Sci USA 83: 5602–5606PubMedCrossRefGoogle Scholar
  63. Rochaix J-D, van Dillewijn J (1982) Transformation of the green alga Chlamydomonas reinhardii with yeast DNA. Nature 296: 70–72PubMedCrossRefGoogle Scholar
  64. Rollo F, Hull R (1982) Liposome-mediated infection of turnip protoplasts with turnip rosette virus and RNA. J Gen Virol 60: 359–363CrossRefGoogle Scholar
  65. Rouze P, Deshayes A, Caboche M (1983) Use of liposomes for the transfer of nucleic acids: Optimization of the method for tobacco mesophyll protoplasts with tobacco mosaic virus RNA. Plant Sci Lett 31: 55–64CrossRefGoogle Scholar
  66. Sheehy RE, Lurquin PF (1983) Targeting of large liposomes with lectins increases their binding to plant protoplasts. Plant Physiol 72: 386–390PubMedCrossRefGoogle Scholar
  67. Shillito RD, Saul MW, Paszkowski J, Muller M, Potrykus I (1985) High efficiency direct gene transfer to plants. Biotechnology 3: 1099–1104CrossRefGoogle Scholar
  68. Sleight MJ (1986) A nonchromatographic assay for expresssion of the chloramphenicol acetyltransferase gene in eukaryotic cells. Anal Biochem 156: 251–256CrossRefGoogle Scholar
  69. Uchimiya H, Fushimi T, Hashimoto H, Harada H, Syono K, Sugarawa Y (1986a) Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Mol Gen Genet 204: 204–207CrossRefGoogle Scholar
  70. Uchimiya H, Hirochika H, Hashimoto H, Hara A, Masuda T, Kasumimoto T, Harada H, Ikeda J-E, Yoshioka M (1986b) Coexpression and inheritance of foreign genes in transformants obtained by direct DNA transformation of tobacco protoplasts. Mol Gen Genet 205: 1–8CrossRefGoogle Scholar
  71. Wang CA, Hughes KW, Huang L (1986) Improved cytoplasmic delivery to plant protoplasts via pH-sensitive liposomes. Plant Physiol 82: 179–184PubMedCrossRefGoogle Scholar
  72. Werr W, Lörz H (1986) Transient gene expression in a Gramineae cell line. Mol Gen Genet 202: 471–475CrossRefGoogle Scholar
  73. Yamaoka N, Furusawa I, Yamamoto M (1982) Infection of turnip protoplasts with cauliflower mosaic virus DNA. Virology 122: 503–505PubMedCrossRefGoogle Scholar
  74. Zaitlin M, Beachy RN (1974) The use of protoplasts and separated cells in plant virus research. In: Lauffer MA, Bang FB, Maramorosch K, Smith KM (eds) Advances in virus research, vol 19. Academic Press, New York, pp 1–35Google Scholar
  75. Zhengkaixu, Luciano CS, Ballard ST, Rhoads RE, Shaw JG (1984) Infection of tobacco protoplasts with liposome-encapsulated potyviral RNA. Plant Sci Lett 36: 137–142CrossRefGoogle Scholar
  76. Jefferson RA (1987) Assaying chimeric genes in plants. The GUS gene fusion system. Plant Molec Biol Rep 5: 387–405CrossRefGoogle Scholar
  77. Klein TM, Wolf ED, Wu R, Sanford JC (1987) High velocity microprojectiles for delivering nucleic acids into living cells. Nature 327: 70–73CrossRefGoogle Scholar
  78. Klein TM, Fromm M, Weissinger A, Tomes D, Schaaf S, Sletten M, Sanford JC (1988) Transfer of foreign genes into intact maize cells with high-velocity microprojectiles. Proc Natl Acad Sci USA 85: 4305–4309PubMedCrossRefGoogle Scholar
  79. Luo ZX, Wu R (1988) A simple method for the transformation of rice via the pollen-tube pathway. Plant Molec Biol Rep 6: 165–174CrossRefGoogle Scholar
  80. McCabe DE, Swain WF, Martinell BJ, Christou P (1988) Stable transformation of soybean (Glycine max) by particle acceleration. Biol. Technol 6: 923–926Google Scholar
  81. Hayes RJ, Petty ITD, Coutts RHA, Buck KW (1988) Gene amplification and expression in plants by a replicating geminivirus vector. Nature 334: 179–182CrossRefGoogle Scholar
  82. Ward A, Etessami P, Stanley J (1988) Expression of a bacterial gene in plants mediated by infectious geminivirus DNA. EMBO J 6: 1583–1587Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • P. F. Lurquin
    • 1
  1. 1.Program in Genetics and Cell BiologyWashington State UniversityPullmanUSA

Personalised recommendations