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Miniprotoplasts and Their Use in Genetic Engineering

  • A. Wallin
  • S. Waara
  • T. Eriksson
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)

Abstract

Protoplasts are an excellent system for the induction of genetic variation in plant cells: through somaclonal variation, somatic hybridization, cybrid formation, transformation and incorporation of isolated chromosomes or isolated DNA. By fragmentation of protoplasts, small-sized, nucleus-containing, nearly vacuole-free subprotoplasts with high stability (miniprotoplasts) can be formed. Due to their small size, the absence of major vacuoles, and their low cytoplasm content, miniprotoplasts have clear advantages over ordinary protoplasts in certain types of cell manipulation.

Keywords

Cytoplasmic Male Sterility Somatic Hybrid Fusion Product Mesophyll Protoplast Percoll Gradient 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • A. Wallin
  • S. Waara
  • T. Eriksson
    • 1
  1. 1.Department of Physiological BotanyUniversity of UppsalaUppsalaSweden

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