Chloroplast Uptake and Genetic Complementation

  • K. L. Giles
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)


Rewriting the chapter more than 10 years after its original publication (Giles 1977) is not made easy by the exciting changes that have occurred in closely associated fields over that time. To a large degree the uptake of foreign DNA, using Agrobacterium tumefaciens as a vector has eclipsed organelle uptake studies in the past few years. This trend is understandable because of the increased flexibility and range of genetic manipulations possible using the Agrobacterium system, since it effectively allows the manipulation of the whole genome rather than the plastoms alone. Chloroplast uptake and exchanges, however, continue to have a useful role in studies involving nuclear-chloroplast interaction, the physiology of isolated plastids and experiments investigating the transfer of herbicide resistance, particularly to the triazines, between varieties and species.


Large Subunit Chloroplast Genome Blue Green Alga Protoplast Fusion Mesophyll Protoplast 
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  1. Alberts AA, Majerus PK, Vagelos PR (1965) Acyl carrier protein VI. Purification and properties of -ketoacyl acyl carrier protein synthetase. Biochemistry 4: 2265–2274CrossRefGoogle Scholar
  2. Armstrong JJ, Surzyki SJ, Moll B, Levine RP (1971) Genetic transcription and translation specifying chloroplast components in Chlamydomonas reinhardi. Biochemistry 10: 692–701PubMedCrossRefGoogle Scholar
  3. Blair GE, Ellis RJ (1972) Light driven synthesis of the large subunit of Fraction I proteins by isolated chloroplasts. Biochem J 127: 42 ppGoogle Scholar
  4. Blair GE, Ellis RJ (1973) Protein synthesis in chloroplasts. I. Light driven synthesis of the large subunit of Fraction I protein by isolated pea chloroplasts. Biochim Biophys Acta 319: 223–234PubMedGoogle Scholar
  5. Bonnett HT, Eriksson T (1974) Transfer of algal chloroplasts into protoplasts of higher plants. Planta 120: 71–79CrossRefGoogle Scholar
  6. Brooks JL, Stumpf PK (1965) A soluble fatty acid synthesizing system from lettuce chloroplasts. Biochim Biophys Acta 98: 210–213Google Scholar
  7. Bucke C, Walker DA, Baldry CW (1966) Some effects of sugars and sugar phosphates on carbon dioxide fixation by isolated chloroplasts. Biochem J 101: 636–641PubMedGoogle Scholar
  8. Burk JG, Grosso JJ (1963) Plasmagenes in variegated tobacco. J Hered 54: 23–25Google Scholar
  9. Carlson PS (1973 ) The use of protoplasts for genetic research. Proc Natl Acad Sci USA 70:598–602PubMedCrossRefGoogle Scholar
  10. Carlson PS ( 1973 ) Towards a parasexual cycle in higher plants. In: Protoplastes et fusion de cellules somatiques végétales. Int Coll Centre Natl Rech Sci, Paris, pp 497–501Google Scholar
  11. Criddle RS, Dau B, Kleinkopf GE, Huffaker RC (1970) Differential synthesis of ribulose diphosphate carboxylase subunits. Biochem Biophys Res Commun 41: 621–627PubMedCrossRefGoogle Scholar
  12. Cseplo A, Medgyesy P, Marton L (1986) In vitro induction, isolation and transfer of chloroplast mutations in Nicotiana. Proc Nuclear techniques and in vitro culture for plant improvement. IAEA, Vienna, pp 137–146Google Scholar
  13. Davey MR, Cocking EC (1972) Uptake of bacteria by isolated higher plant protoplasts. Nature (Lond) 239:455–456CrossRefGoogle Scholar
  14. Eisenstadt JM, Brawerman G (1963) The incorporation of amino acids into the protein of chloroplasts and chloroplast ribosomes of Euglena gracilis. Biochim Biophys Acta 76: 319–321PubMedCrossRefGoogle Scholar
  15. Eisenstadt JM, Brawerman G (1964) The protein synthesizing systems from the cytoplasm of Euglena gracilis. J Mol Biol 10: 392–402PubMedCrossRefGoogle Scholar
  16. Enzmann-Becker G (1973) Plating efficiency of protoplasts of tobacco in different light conditions. Z Naturforsch 28: 470–471Google Scholar
  17. Giles KL (1972) An interspecific aggregate cell capable of cell wall regeneration. Plant Cell Physiol 13: 207–210Google Scholar
  18. Giles KL (1973) Attempts to demonstrate genetic complementation by the technique of protoplast fusion. In: Protoplastes et fusion de cellules somatiques végétales. Coll Int Centre Natl Rech Sci, Paris, pp 485–495Google Scholar
  19. Giles KL (1974) Complementation by protoplast fusion using mutant strain of maize. Plant Cell Physiol 15: 281–285Google Scholar
  20. Giles KL (1977) Chloroplast uptake and genetic complementation. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue and organ culture. Springer, Berlin Heidelberg New York, pp 536–550Google Scholar
  21. Giles KL, Sarafis V (1971) On the survival and reproduction of chloroplasts outside the cell. Cytobiosynthesis 4: 61–74Google Scholar
  22. Giles KL, Sarafis V (1972) Chloroplast survival and division in vitro. Nature New Biol 236: 56–58PubMedGoogle Scholar
  23. Giles KL, Sarafis V (1974) Implications of rigescent integuments as a new structural feature of some algal chloroplasts. Nature (Lond) 248: 512CrossRefGoogle Scholar
  24. Giles RE, Ruddle FH (1973) Production and characterisation of proliferating somatic cell hybrids. In: Kruse PF, Patterson MK (eds) Tissue culture-methods and applications. Academic Press, New York London, pp 475–500Google Scholar
  25. Glimelius K, Bonnett HT (1986) Nicotiana cybrids with Petunia chloroplasts. Theor Appl Genet 72: 794–798CrossRefGoogle Scholar
  26. Glimelius K, Chen K, Bonnett HT (1981) Somatic hybridization in Nicotiana: Segregation of organellar traits among hybrid and cybrid progeny. Planta 153: 504–510CrossRefGoogle Scholar
  27. Hess D (1969a) Versuche zur Transformation an höheren Pflanzen: Induktion und konstante Weitergabe der Anthocyansynthese bei Petunia hybrida. Z Pflanzenphysiol 60: 348–353Google Scholar
  28. Hess D (1969b) Versuche zur Transformation an höheren Pflanzen: Wiederholung der Anthocyan-Induktion bei Petunia und erste Charakterisierung des transformierenden Prinzips. Z Pflanzenphysiol 61: 286–298Google Scholar
  29. Jenkins MT (1924) Heritable characteristics of maize XX. Iojap striping a chlorophyll defect. J Hered 15: 472–497Google Scholar
  30. Kao KN, Michayluk MR (1974) A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115: 355–367CrossRefGoogle Scholar
  31. Kawaguti S, Yamasu T (1965) Electron microscopy on the symbiosis between an elypsioid gastropod and chloroplasts of a green alga. Biol J Okayama Univ 11: 57–62Google Scholar
  32. Kawashima N (1969) Comparative studies from Fraction I protein from spinach and tobacco leaves. Plant Cell Physiol 10: 31–40Google Scholar
  33. Kawashima N (1970) Non-synchronous incorporation of 14CO2 into amino acids of the two subunits of Fraction I protein. Biochem Biophys Res Commun 38: 119–124PubMedCrossRefGoogle Scholar
  34. Kawashima N, Wildman SG (1970) Fraction I protein. Annu Rev Plant Physiol 21: 325–358CrossRefGoogle Scholar
  35. Kawashima N, Wildman SG (1971) Studies on Fraction I protein. II. Comparison of physical, chemi-cal, immunological and enzymatic properties between spinach and tobacco. Fraction I protein. Biochim Biophys Acta 229: 749–760PubMedGoogle Scholar
  36. Kawashima N, Wildman SG (1972) Studies on Fraction I protein. IV. Mode of inheritance of primary structure in relation to whether chloroplast or nuclear DNA contains code for a chloroplast protein. Biochim Biophys Acta 262: 42–49PubMedGoogle Scholar
  37. Kawashima N, Kwok S, Wildman SG (1971) Studies on Fraction I protein. III. Comparison of the primary structure of the large and small subunits obtained from five species of Nicotiana. Biochim Biophys Acta 236: 578–586PubMedGoogle Scholar
  38. Kirk JTO, Tilney-Bassett RAE (1967) The Plastids. Freeman, London San FranciscoGoogle Scholar
  39. Ledoux L, Huart R (1968) Integration and replication of DNA of Micrococcus lysodeikticus in DNA of germinating barley. Nature (Lond) 218: 1256–1259CrossRefGoogle Scholar
  40. Lyttleton JW, Ts’o POP (1958) The localisation of Fraction I protein of green leaves in the chloroplasts. Arch Biochem Biophys 73: 120–126PubMedCrossRefGoogle Scholar
  41. Maliga P (1985) Cell culture isolation and characterization of agronomically useful mutants of higher plants. Biotechnology in international agricultural research; inter-center seminar on international agricultural research centers and biotechnology, Manila, Philippines, 1984, pp 111–120Google Scholar
  42. McClintock B (1941) The stability of broken ends of chromosome in Zea mays. Genetics 26: 234–282PubMedGoogle Scholar
  43. McClintock B (1944) The relation of homozygous deficiencies to mutations and allelic series in maize. Genetics 29: 478–502PubMedGoogle Scholar
  44. McFadden BA (1973) Autotrophic CO2 assimilation and the evolution of ribulose diphosphate carboxylase. Bacteriol Rev 37: 289–319PubMedGoogle Scholar
  45. Medgyesy P, Golling R, Nagy F (1985) A light sensitive recipient for the effective transfer of chloroplast and mitochondrial traits by protoplast fusion in Nicotiana. Theor Appl Genet 70: 590–594CrossRefGoogle Scholar
  46. Meeks JC, Malmberg RL, Wolk PC (1978) Uptake of auxotrophic cells of a heterocyst-forming Cyanobacterium by tobacco protoplasts and the fate of their associations. Planta 139: 55CrossRefGoogle Scholar
  47. Melchers G, Labib G (1974) Somatic hybridisation of plants by fusion of protoplasts. I. Selection of light resistant hybrids of “haploid” light sensitive varieties of tobacco. Mol Gen Genet 135: 277–294CrossRefGoogle Scholar
  48. Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100: 43–52Google Scholar
  49. Moon KE, Thompson EOP (1969) Subunits from reduced and 5-carboxymethylated ribulose diphosphate carboxylase ( Fraction I protein ). Austr J Biol Sci 22: 463–470Google Scholar
  50. Mudd JB, McManus TT (1965) Relationship of the synthesis of lipid and water soluble acids by chloroplast preparations. Plant Physiol 40: 340–344PubMedCrossRefGoogle Scholar
  51. Nass MMK (1969) Uptake of isolated chloroplasts by mammalian cells. Science 165:1128–1131PubMedCrossRefGoogle Scholar
  52. Nolla JAB (1934) A study of glaucous and yellow characters in Nicotiana tabacum L. J Agric Univ Puerto Rico 18: 443–562Google Scholar
  53. Park RB, Pon NG (1961) Correlation of structure with function in Spinacea oleracea chloroplasts. J Mol Biol 3: 1–10PubMedCrossRefGoogle Scholar
  54. Potrykus I (1973) Transplantation of chloroplasts into protoplasts of Petunia. Z Pflanzenphysiol 70: 364–366Google Scholar
  55. Potrykus I, Hoffmann F (1973) Transplantation of nuclei into protoplasts of higher plants. Z Pflanzenphysiol 69: 287–289Google Scholar
  56. Povilaitis B, Cameron DR (1963) A mutation causing chlorophyll deficiency in Nicotiana tabacum. Can J Genet Cytol 5: 233–238Google Scholar
  57. Rhoades HH (1943) Genic induction of an inherited cytoplasmic difference. Proc Natl Acad Sci USA 29: 327–329PubMedCrossRefGoogle Scholar
  58. Ridley SD, Leech RM (1970) Division of chloroplasts in an artificial environment. Nature (Lond) 227: 463–465CrossRefGoogle Scholar
  59. Rutner AC, Lave MD (1967) Non-identical subunits of ribulose diphosphate carboxylase Biochim Biophys Res Commun 28: 531–537Google Scholar
  60. Rutner AC (1970) Spinach-D 5-phosphoribose isomerase purification and properties of the enzyme. Biochemistry 9 (1): 178–184PubMedCrossRefGoogle Scholar
  61. Salts Y, Hermann RG, Peleg N, Lavi U, Izhar F, Frankel R, Beckmann JS (1984) Physical mapping of plastid DNA varieties among eleven Nicotiana species. Theor Appl Genet 69: 1–14CrossRefGoogle Scholar
  62. Shell JS (1987) Transgenic plants as tools to study the molecular organization of plant genes. Science 237: 1176–1182CrossRefGoogle Scholar
  63. Shvidkaya LG, Gleba YY (1974) Plating of mesophyll protoplasts, isolated from different photosynthetic mutants of Nicotiana tabacum. L. 3rd Int Congr Plant cell and tissue culture, Abstr 195, Univ LeicesterGoogle Scholar
  64. Smith HH (1972) Plant genetic tumors. Progr Exp Tumor Res 15: 138–164PubMedGoogle Scholar
  65. Smith HH (1974) Model systems for somatic cell plant genetics. BioScience 24: 269–276CrossRefGoogle Scholar
  66. Smith HH (1975) Nicotiana. In: King RC (ed) Handbook of genetics. Plenum, LondonGoogle Scholar
  67. Stadler LJ (1946) Spontaneous mutation of the R locus of maize. I. The aleurone colour and plant colour effects. Genetics 31: 377–394Google Scholar
  68. Stubbe W (1957) Dreifarbenpanaschierung bei Oenothera. I. Entmischung von 3 in der Zygote vereinigten Plastidomen. Ber Dtsch Bot Ges 70: 221–226Google Scholar
  69. Sugiyama T, Tomoki I, Azakawa T (1971) Subunit structure of ribulose 1,5-diphosphate carboxylase from Chlorella ellipsoidea. Biochemistry 10: 3406–3411PubMedCrossRefGoogle Scholar
  70. Takebe I, Otsuki Y (1969) Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proc Natl Acad Sci USA 64: 843–848PubMedCrossRefGoogle Scholar
  71. Takebe I, Otsuki Y (1973) Fine structure of isolated mesophyll protoplasts of tobacco. Planta 113: 21–27CrossRefGoogle Scholar
  72. Takebe I, Otsuki Y (1974) Multiple infection of tobacco mesoplasts by plant viruses. 3rd Int Congr Plant cell and tissue culture, Abstr 36, Univ LeicesterGoogle Scholar
  73. Takebe I, Otsuki Y, Aoki S (1968) Isolation of tobacco mesophyll cells in intact and active state. Plant Cell Physiol 9: 115–124Google Scholar
  74. Takebe I, Labib G, Melchers G (1971) Regeneration of whole plants from isolated mesophyll protoplasts of tobacco. Naturwissenschaften 58: 318–320CrossRefGoogle Scholar
  75. Taylor DL (1968) Chloroplasts as symbiotic organelles in the digestive gland of Elysia viridis (Gastropoda: Opisthobrancha). J Mar Biol 48: 1–15CrossRefGoogle Scholar
  76. Tolbere NE (1958) Secretion of glycolic acid by chloroplasts. In: The photochemical apparatus. Its structure and function. Brookhaven Symp Biol 11: 271–275Google Scholar
  77. Trench RK (1969) Chloroplasts as functional endosymbionts in the mollusc Tridachia crispata (Bergh). Nature (Lund) 222: 1071–1072CrossRefGoogle Scholar
  78. Trench RK, Green RW, Bystrom BG (1969) Chloroplasts as functional organelles in animal tissues. J Cell Biol 42: 404–407PubMedCrossRefGoogle Scholar
  79. Valleau WD (1957) Yellow Crittenden-a mutant in dark tobacco. Tab Sci 1: 91–92Google Scholar
  80. Wallin A, Glimelius K (1974) The induction of aggregation and fusion of Daucus carota protoplasts by polyethylene glycol. Z Pflanzenphys 74 (1): 64–80Google Scholar
  81. Weissbach A, Horecker BL, Hurwitz J (1956) The enzyme formation of phosphoglyceric acid from ribulose diphosphate and carbon dioxide. J Biol Chem 218: 795–810PubMedGoogle Scholar
  82. Wildman SG, Bonner J (1974) Proteins of green leaves. I. Isolation, enzymic properties and auxin content of spinach cytoplasmic proteins. Arch Biochem Biophys 14: 381–413Google Scholar
  83. Wisselingh C van (1920) Über Variabilität and Erblichkeit. Z Indukt Abst Vererbungsl 22:65 Yamada T, Sakaguchi K (1981) Polyethylene glycol induced uptake of bacteria into yeast protoplasts. Agric Biol Chem 45: 2301–2309Google Scholar
  84. Yamada T, Sakaguchi K (1981) Polyethylene glycol induced uptake of bacteria into yeast protoplasts. Agric Biol Chem 45:2301–2309CrossRefGoogle Scholar
  85. Yonge CM, Nicholas HM (1940) Structure and function of the gut and symbiosis with zooxanthellae in Tridachia crispata. Pap Tortugas Lab 32: 289–303Google Scholar
  86. Zelcer A, Aviv D, Galun E (1978) Interspecific transfer of cytoplasmic male sterility by fusion between protoplasts of normal Nicotiana sylvestris and x-ray irradiated protoplasts of male sterile N. tabacum. Z Pflanzenphysiol 90: 397–407Google Scholar
  87. Zucker (1972) Light and enzymes. Annu Rev Plant Physiol 23: 133–156CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • K. L. Giles
    • 1
  1. 1.c/o Plant Biotechnology Institute, National Research Council of CanadaVipont Research Laboratories (Canada) Inc.SaskatoonCanada

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