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Flow Cytometric Analysis and Sorting of Somatic Hybrid and Transformed Protoplasts

  • D. W. Galbraith
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)

Abstract

Considerable interest has developed in the potential of techniques of flow cytometry and cell sorting for providing insight into the processes of plant growth and development (Brown 1984; Galbraith 1984a, b; Petit et al. 1986; Glimelius 1987). The initial technical advance that culminated in the application of flow cytometry to plant cell systems was the development of procedures for the isolation of protoplasts from a wide variety of plant tissues. This is because a fundamental requirement for the operation of flow cytometers is the availability of suspensions of cells having isometric shapes. As detailed below, flow cytometry involves the analysis of the fluorescence and light-scattering properties of single cells during their passage within a narrow, precisely defined liquid stream through the focus of an intense light source. With the exception of pollen and microspores, protoplasts remain the sole example, within higher plant genera, of single-cell suspensions that are suitable for flow cytometric analysis.

Keywords

Flow Cytometric Analysis Somatic Hybrid Protoplast Fusion Plant Protoplast Leaf Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • D. W. Galbraith
    • 1
  1. 1.Department of Plant SciencesUniversity of ArizonaTucsonUSA

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