Electrofusion: The Technique and Its Application to Somatic Hybridization
The use of electric fields for inducing protoplast fusion (electrofusion) has its origins in biophysical studies of cell membranes. However, recent work showing electrofusion to be simpler, quicker, and often more efficient than chemically induced fusion is attracting the interest of geneticists and tissue-culture practitioners. Indeed, electrofusion may become the fusion technique of choice for species whose cells exhibit a severe cytotoxic response to polyethylene glycol (PEG). Interest in this area has been further heightened by recent demonstrations that electric pulses can be used to introduce foreign DNA into plant cells (“electroporation”: Fromm et al. 1986; Riggs and Bates 1986). This chapter provides an overview of electrofusion and its application to plant somatic hybridization.
KeywordsSomatic Hybridization Fusion Product Mesophyll Protoplast Plant Protoplast Protoplast Suspension
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