Microinjection of Cells and Protoplasts: Integration of Foreign DNA

  • A. Crossway
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)


A number of plant transformation techniques are currently available, none of which has proven to be universally applicable. These methods include Agrobacterium-mediated gene transfer (Nester et al. 1984), direct DNA uptake via chemical mediation (Krens and Schilperoort 1984), liposome fusion (Deshayes et al. 1985) or electroporation (Fromm et al. 1986), and direct DNA microinjection (Crossway et al. 1986c; Reich et al. 1986c). These methods are currently limited by either the host-range restrictions of Agrobacterium or by the species restrictions on plant regeneration from protoplasts. These barriers are gradually being overcome; the most notable example is, perhaps, the regeneration of rice from protoplasts (Coulibaly and Demarly 1986; Yamada et al. 1986; Abdullah et al. 1986; Toriyama et al. 1986; also see Kyozuka et al. 1989). Transformation techniques are now being applied to a variety of regenerable plant cell types (especially those with intact cell walls) in order to broaden the range of plant species which can be transformed. As both the transformation and cell culture technologies advance (see Bajaj 1986), a repertoire of plant transformation technologies will be built that will allow selection of the method most suited to a particular cell type or plant species based on the efficiency of transformation.


Plant Protoplast Southern Hybridization Analysis High Transformation Frequency Plant Cell Transformation Tobacco Mesophyll Protoplast 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • A. Crossway
    • 1
  1. 1.Calgene IncorporationDavisUSA

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