Direct Gene Transfer in Protoplasts of Nicotiana plumbaginifolia
Different methodologies to transfer foreign genes into higher plants have recently been developed. These include: (1) insertion of the gene into the T-DNA region of the Ti plasmid of Agrobacterium tumefaciens (or of the Ri plasmid of A. rhizogenes) followed by infection of tissues (Gheysen et al. 1985); (2) direct gene transfer from bacterial plasmids to protoplasts (Paszkowski et al. 1984; Shillito et al. 1985); (3) insertion of the gene into a plant virus used to infect plants (Brisson et al. 1984); (4) microinjection of plasmid or nuclear DNA into plant nuclei (Reich et al. 1986); (5) injection of DNA into young floral tillers (De La Pena et al. 1987); (6) delivery of DNA into plant cells using high-velocity microprojectiles (Klein et al. 1987).
KeywordsProtoplast Isolation Protoplast Culture Zeatine Riboside Leaf Protoplast Direct Gene Transfer
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- Gheysen G, Dhaese P, Van Montagu M, Schell J (1985) DNA flux across genetic barriers: the crown gall phenomenon. In: Hohn B, Dennis E (eds) Plant Gene Research. Genetic flux in plants. Springer, Vienna, pp 12–47Google Scholar
- Negrutiu I, Shillito RD, Potryus I, Biasini MG, Sala F (1987) Hybrid genes in the analysis of trans-formation conditions. I. Setting up a simple method for direct gene transfer in plant protoplasts.Plant Mol Biol 8:363 —373Google Scholar
- Paszkowsky J, Shillito RD, Saul MW, Mandak V, Hohn J, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3: 2717–2722Google Scholar
- Potrykus I, Shillito RD, Saul MW, Paszkowski J (1985) Direct gene transfer. State of the art and future potential. Plant Mol Biol Rep 3: 117–128Google Scholar
- Reich TJ, Iyer VN, Miki BL (1986) Efficient transformation of alfalfa protoplasts by the intranuclear microinjection of Ti plasmids. Biotechnology 4:1001–1004Google Scholar