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Direct Gene Transfer in Protoplasts of Nicotiana plumbaginifolia

  • F. Sala
  • M. L. Marchesi
  • S. Castiglione
  • J. Paszkowski
  • M. Saul
  • I. Potrykus
  • I. Negrutiu
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)

Abstract

Different methodologies to transfer foreign genes into higher plants have recently been developed. These include: (1) insertion of the gene into the T-DNA region of the Ti plasmid of Agrobacterium tumefaciens (or of the Ri plasmid of A. rhizogenes) followed by infection of tissues (Gheysen et al. 1985); (2) direct gene transfer from bacterial plasmids to protoplasts (Paszkowski et al. 1984; Shillito et al. 1985); (3) insertion of the gene into a plant virus used to infect plants (Brisson et al. 1984); (4) microinjection of plasmid or nuclear DNA into plant nuclei (Reich et al. 1986); (5) injection of DNA into young floral tillers (De La Pena et al. 1987); (6) delivery of DNA into plant cells using high-velocity microprojectiles (Klein et al. 1987).

Keywords

Protoplast Isolation Protoplast Culture Zeatine Riboside Leaf Protoplast Direct Gene Transfer 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • F. Sala
    • 1
    • 2
  • M. L. Marchesi
    • 2
  • S. Castiglione
    • 2
  • J. Paszkowski
    • 3
  • M. Saul
    • 3
  • I. Potrykus
    • 3
  • I. Negrutiu
    • 4
  1. 1.Department of BotanyUniv. of ParmaParmaItaly
  2. 2.Department of Genetics and MicrobiologyUniv. of PaviaPaviaItaly
  3. 3.Institut für PflanzenwissenschaftenETH-ZentrumZürichSwitzerland
  4. 4.Instituut voor Moleculaire BiologieSaint Genesius-RodeBelgium

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