Abstract
Higher plant cells have firm cell walls that have been obstacles for cell manipulations. The method for removing cell walls by commercial enzymes was developed (Takebe et al. 1968). The spheres of cytoplasm isolated as a result were called protoplasts. Protoplasts are also obtained from Gram-positive or -negative bacteria by lysozyme, an enzyme for digesting bacterial cell walls. However, in many cases of Gram-negative bacteria, lysozyme treatments yield incomplete bacterial protoplasts accompanied by the remains of cell walls, designated spheroplasts (Bird-sell and Cota-Robles 1967).
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© 1989 Springer-Verlag Berlin Heidelberg
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Hasezawa, S., Syōno, K. (1989). Transformation in Vinca rosea L.: An Example of Spheroplast Method for Gene Transfer. In: Bajaj, Y.P.S. (eds) Plant Protoplasts and Genetic Engineering II. Biotechnology in Agriculture and Forestry, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74454-9_10
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DOI: https://doi.org/10.1007/978-3-642-74454-9_10
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