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Transformation in Vinca rosea L.: An Example of Spheroplast Method for Gene Transfer

  • S. Hasezawa
  • K. Syōno
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)

Abstract

Higher plant cells have firm cell walls that have been obstacles for cell manipulations. The method for removing cell walls by commercial enzymes was developed (Takebe et al. 1968). The spheres of cytoplasm isolated as a result were called protoplasts. Protoplasts are also obtained from Gram-positive or -negative bacteria by lysozyme, an enzyme for digesting bacterial cell walls. However, in many cases of Gram-negative bacteria, lysozyme treatments yield incomplete bacterial protoplasts accompanied by the remains of cell walls, designated spheroplasts (Bird-sell and Cota-Robles 1967).

Keywords

Crown Gall Plant Protoplast Octo Pine Mannitol Solution Lysozyme Treatment 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Baba A, Hasezawa S, Syōno K (1986) Cultivation of rice protoplasts and their transformation mediated by Agrobacterium spheroplasts. Plant Cell Physiol 27: 463–471Google Scholar
  2. Birdsell DC, Cota-Robles EH (1967) Production and ultrastructure of lysozyme and ethylenediaminetetraacetate-lysozyme spheroplasts of Escherichia coli. J Bacteriol 93: 427–437PubMedGoogle Scholar
  3. Chilton M-D, Currier TC, Ferrand SK, Bendich AJ, Gordon MP, Nester EW (1974) Agrobacterium tumefaciens DNA and PS8 bacteriophage DNA not detected in crown gall tumors. Proc Natl Acad Sci USA 71: 3672–3676Google Scholar
  4. Fraser D, Jerrel EA (1953) The amino acid composition of T3 bacteriophage. J Biol Chem 205:291 — 295Google Scholar
  5. Freeman JP, Draper J, Davey MR, Cocking EC, Gartland KMA, Hardring K, Pental D (1984) A comparison of methods for plasmid delivery into plant protoplasts. Plant Cell Physiol 25:1353 —1365Google Scholar
  6. Hain R, Steinbiß H-H, Shell J (1984) Fusion of Agrobacterium and E. coli spheroplasts with Nicotiana tabacum protoplasts. Direct gene transfer from microorganism to higher plant. Plant Cell Rep 3:60 —64Google Scholar
  7. Hasezawa S, Nagata T, Syōno K (1981) Transformation of Vinca protoplasts mediated by ilgrobacterium spheroplasts. Mol Gen Genet 182: 206–210CrossRefGoogle Scholar
  8. Hasezawa S, Matsui C, Nagata T, Syōno K (1985) Cytological study of the introduction of Agrobacterium tumefaciens spheroplasts into Vinca rosea protoplasts. Can J Bot 61: 1052–1057CrossRefGoogle Scholar
  9. Kao KN, Michayluk MR (1974) A method for high-frequency intergeneric fusion of plant protoplasts. Planta 115: 355–367CrossRefGoogle Scholar
  10. Kingsman AJ, Clarke L, Mortimer RK, Carbon J (1979) Replication in Saccharomyces cerevisiae of plasmid pBR 313 carrying DNA from the yeast trp 1 region. Gene 12: 139–146Google Scholar
  11. Kuroiwa T, Suzuki T (1980) An improved method for the demonstration of in situ chloroplast nuclei in higher plants. Cell Struct Funct 5:195 —197Google Scholar
  12. Marton L, Wullems GJ, Molendijk L, Schilperoort RA (1979) In vitro transformation of cultured cells from Nicotiana tabacum by Agrobacterium tumefaciens. Nature 277: 129–131CrossRefGoogle Scholar
  13. Matsui C, Hasezawa S, Tanaka N, Syōno K (1983) Introduction of Escherichia coli cells and spheroplasts into Vinca protoplasts. Plant Cell Rep 2: 30–32Google Scholar
  14. Millman RA, Lurquin PF (1983) Study of plant protoplast-bacterial spheroplast and cell interaction by flow cytometry. J Plant Physiol 117: 431–440Google Scholar
  15. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  16. Nagata T (1978) A novel cell-fusion method of protoplasts by polyvinyl alcohol. Naturwis: enschaften 65:263 —264Google Scholar
  17. Okada K, Hasezawa S, Syōno K, Nagata T (1985) Further evidence for the transformation of Vinca rosea protoplasts by Agrobacterium tumefaciens spheroplasts. Plant Cell Rep 4:133 —136Google Scholar
  18. Otten LABM, Schilperoort RA (1978) A rapid microscale method for the detection of lysopine dehydrogenase activities. Biochim Biophys Acta 525:497 — 500Google Scholar
  19. Rassoulzadegan M, Binétruy B, Cuzin F (1982) High frequency of gene transfer after fusion between bacteria and eukaryotic cells. Nature 295: 257–259PubMedCrossRefGoogle Scholar
  20. Sandri-Goldin RM, Goldin AL, Levine M, Glorioso JC (1981) High frequency transfer of cloned herpes simplex virus type 1 sequences to mammalian cells by protoplast fusion. Mo: Cell Biol 8:743 — 752Google Scholar
  21. Schaffner W (1980) Direct transfer of cloned genes from bacteria to mammalian cells. Proc Natl Acad Sci USA 77:2163 —2167Google Scholar
  22. Syōno K, Hasezawa S (1985) Spheroplast — As a tool of gene transfer. In: Cheremisinoff PN, Ouellette RP (eds) Biotechnology — Application and research. Technomic Pub, Lancaster: 463 — 476Google Scholar
  23. Takebe I, Otsuki Y, Aoki S (1968) Isolation of tobacco mesophyll cells in intact and active state. Plant Cell Physiol 9:115 —124Google Scholar
  24. Tanaka N, Fukunaga Y, Hasezawa S, Syōno K, Matsui C (1984) Endocytic uptake of Escherichia coli spheroplasts by Neurospora crassa slime cells. — An ultrastructural study. Appl Microbial Biotechnol 19: 296–299CrossRefGoogle Scholar
  25. Tanaka N, Ikegami M, Hohn T, Matsui C, Watanabe I (1984) E. coli spheroplast-mediated transfer of cloned cauliflower mosaic virus DNA into plant protoplasts. Mol Gen Genet 195: 378–380Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • S. Hasezawa
  • K. Syōno
    • 1
  1. 1.Department of Pure and Applied Sciences, College of Arts and SciencesUniversity of TokyoKomaba, Meguro-ku, Tokyo, 153Japan

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