Transformation in Vinca rosea L.: An Example of Spheroplast Method for Gene Transfer

  • S. Hasezawa
  • K. Syōno
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)


Higher plant cells have firm cell walls that have been obstacles for cell manipulations. The method for removing cell walls by commercial enzymes was developed (Takebe et al. 1968). The spheres of cytoplasm isolated as a result were called protoplasts. Protoplasts are also obtained from Gram-positive or -negative bacteria by lysozyme, an enzyme for digesting bacterial cell walls. However, in many cases of Gram-negative bacteria, lysozyme treatments yield incomplete bacterial protoplasts accompanied by the remains of cell walls, designated spheroplasts (Bird-sell and Cota-Robles 1967).


Crown Gall Plant Protoplast Octo Pine Mannitol Solution Lysozyme Treatment 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • S. Hasezawa
  • K. Syōno
    • 1
  1. 1.Department of Pure and Applied Sciences, College of Arts and SciencesUniversity of TokyoKomaba, Meguro-ku, Tokyo, 153Japan

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