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Genetic Engineering and In Vitro Manipulation of Plant Cells — Technical Advances

  • Y. P. S. Bajaj
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)

Abstract

It is envisaged that the next agricultural revolution will be through in vitro technology. Tremendous progress made during the last decade has amply demonstrated that refinement of routine in vitro culture techniques and development of new biotechnologies such as somatic cell hybridization, transformation through Agrobacterium, direct gene transfer, electrofusion, electroporation, flow cytometry, uptake of nuclei, chromosomes, DNA, chloroplasts, bacteria, viruses, fungi, and the transfer of mitochondria by protoplasts and other recombinant DNA techniques have enabled the opening of new vistas for plant improvement (Potrykus et al. 1985 a; Bajaj 1986, 1989; Crossway et al. 1986a; Perani et al. 1986; Cocking and Davey 1987; Schell 1987). Protoplasts have been shown to be excellent tools for genetic manipulation, since entire plants can be regenerated from them under suitable culture conditions — a prerequisite for genetic manipulation studies. The subject of isolation and culture of protoplasts has already been dealt with in detail and now there are a large number of species in which entire plants can be routinely regenerated (see Bajaj 1989).

Keywords

Cytoplasmic Male Sterility Somatic Hybrid Protoplast Fusion Agrobacterium Rhizogenes Petunia Hybrida 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Abel PP, Nelson RS, De B, Hoffmann N, Rogers SG, Fraley RT, Bachy RN (1986) Delay of disease development in transgenic plants that express the tobacco mosaic virus coat protein gene. Science 232: 738–743PubMedCrossRefGoogle Scholar
  2. Afonso CL, Harkins KR, Thomas-Compton MA, Krejci AE, Galbraith DW (1985) Selection of somatic hybrid plants in Nicotiana through fluorescence-activated sorting of protoplasts. Biotechnology 3: 811–816CrossRefGoogle Scholar
  3. Alexander RG, Cocking EC, Jackson PJ, Jett JH (1985) The characterization and isolation of plant heterokaryons by flow cytometry. Protoplasma 128: 52–58CrossRefGoogle Scholar
  4. Anderson LK, Stack SM, Fox MH, Chuanshan Z (1985) The relationship between genome size and synaptonemal complex length in higher plants. Exp Cell Res 156: 367–378PubMedCrossRefGoogle Scholar
  5. Archer EK, Landgren CR, Bonnett HT (1982) Cytoplast formation and enrichment from mesophyll tissues of Nicotiana spp. Plant Sci Lett 25: 175–185CrossRefGoogle Scholar
  6. Armstead IP, Webb KJ (1987) Effect of age and genotype of tissue on genetic transformation of Lotus corniculatus by Agrobacterium tumefaciens. Plant Cell Tissue Organ Cult 9: 95–101CrossRefGoogle Scholar
  7. Asahi T, Kumashiro T, Kubo T (1988) Constitution of mitochondrial and chloroplast genomes in male sterile tobacco obtained by protoplast fusion of Nicotiana tabacum and N. debneyi. Plant Cell Physiol 29: 43–49Google Scholar
  8. Ashcroft RG, Preston C, Cleland RE, Critchley C (1986) Flow cytometry of isolated chloroplasts and thylakoids. Photobiochem Photobiophys 13: 1–14Google Scholar
  9. Austin S, Ehlenfeld MK, Baer MA, Helgeson JP (1986) Somatic hybrids produced by protoplast fusion between Solanum tuberosum and S. brevidens: phenotypic variation under field conditions. Theor Appl Genet 71: 682–690CrossRefGoogle Scholar
  10. Aviv D, Galun E (1986) Does pretreatment by rhodamine 6-G affect the mitochondrial composition of fusion-derived Nicotiana cybrids? Plant Cell Rep 3: 227–230CrossRefGoogle Scholar
  11. Aviv D, Bleichman S, Arzee-Gonen P, Galun S (1984a) Interspecific cytoplasmic hybrids in Nicotiana. Identification of plastomes and chondriomes in N sylvestris+N. rustica cybrids having N. sylves-tris nuclear genomes. Theor Appl Genet 67: 499–504CrossRefGoogle Scholar
  12. Aviv D, Arzee-Gonen P, Bleichman S, Galun E (1984b) Novel alloplasmic Nicotiana plants by “donor-recipient” protoplast fusion: cybrids having N tabacum or N. sylvestris nuclear genomes and either or both plastomes and chondriomes from alien species. Mol Gen Genet 196: 244–253CrossRefGoogle Scholar
  13. Ayers NM (1987) Inheritance of organelles in somatic cell hybrids and cybrids of Nicotiana. Ph D Thesis, Univ Nebraska, LincolnGoogle Scholar
  14. Bajaj YPS (1974) Isolation and culture studies on pollen tetrad and pollen mother cell protoplasts. Plant Sci Lett 3: 93–99CrossRefGoogle Scholar
  15. Bajaj YPS (1983) Haploid protoplasts. In: Giles KL (ed) Plant protoplasts. Academic Press, New York London, pp 113–141Google Scholar
  16. Bajaj YPS (ed) (1986) Biotechnology in agriculture and forestry, vol 2. Crops I. Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  17. Bajaj YPS (1987) In vitro biotechnologies for crop improvement. Int Symp Worksh Gene manipulation for plant improvement in developing countries, Kuala LumpurGoogle Scholar
  18. Bajaj YPS (ed) (1989) Biotechnology in agriculture and forestry, vol 8. Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  19. Bajaj YPS, Gill MS, Gosal SS (1987) Somatic hybridization — a biotechnological approach to plant breeding. In: Ganguli PK, Mandal AK (eds) Advances in plant breeding methods. Oxford and IBH Press, New DelhiGoogle Scholar
  20. Baldes R, Moos M, Geider K (1987) Transformation of soybean protoplasts from permanent suspen-sion cultures by cultivation with cells of Agrobacterium tumefaciens. Plant Mol Biol 9: 135–146CrossRefGoogle Scholar
  21. Barsby TL, Yarrow SA, Kemble RJ, Grant I (1987) The transfer of cytoplasmic male sterility to winter-type oilseed rape (Brassica napus L.) by protoplast fusion. Plant Sci 53: 243–248CrossRefGoogle Scholar
  22. Basiran N, Armitage P, Scott R, Draper J (1987) Genetic transformation of flax (Linum usitatis-simum) by Agrobacterium tumefaciens: Regeneration of transformed shoots via a callus phase. Plant Cell Rep 6: 396–399CrossRefGoogle Scholar
  23. Bates GW (1985) Electric fusion for optimum formation of protoplast heterokaryons in Nicotiana. Planta 165: 217–224CrossRefGoogle Scholar
  24. Belliard G, Vedel F, Pelletier G (1979) Mitochondrial recombination in cytoplasmic hybrids of Nicotiana tabacum by protoplast fusion. Nature (London) 281: 401–403CrossRefGoogle Scholar
  25. Bergounioux C, Perennes C, Miege C, Gadal P (1986) The effect of male sterility on protoplast divi-sion in Petunia hybrida. Cell cycle comparison by flow cytometry. Protoplasma 130: 138–144CrossRefGoogle Scholar
  26. Bilkey PC, Davey MR, Cocking EC (1982) Isolation, origin and properties of enucleated plant microplasts. Protoplasma 110: 147–151CrossRefGoogle Scholar
  27. Binding H, Jain SM, Fuiger J, Mordhorst G, Nehls R, Gressel J (1982) Somatic hybridization of an atrazine resistant biotype of Solanum nigrum with Solanum tuberosum. Theor Appl Genet 63: 273–277CrossRefGoogle Scholar
  28. Boeshore ML, Hanson MR, Izhar S (1985) A variant mitochondrial DNA arrangement specific to Petunia stable sterile somatic hybrids. Plant Mol Biol 4: 125–132CrossRefGoogle Scholar
  29. Bonnett HT, Eriksson T (1974) Transfer of algal chloroplasts into protoplasts of higher plants. Planta 120: 71–79CrossRefGoogle Scholar
  30. Bourgin JP, Missonier C, Goujaud J (1986) Direct selection of cybrids by streptomycin and valine resistance in tobacco. Theor Appl Genet 72: 11–14CrossRefGoogle Scholar
  31. Bradley PM (1978) Production of enucleated plant protoplasts of Allium cepa. Plant Sci Lett 13: 287–290CrossRefGoogle Scholar
  32. Braun AC (1943) Studies on tumor inception in crown gall disease. Am J Bot 30: 674–677CrossRefGoogle Scholar
  33. Brisson N, Paszkowski J, Penswick JR, Gronenborn B, Potrykus I, Hohn T (1984) Expression of a bacterial gene in plants using a viral vector. Nature (London) 310: 511–514CrossRefGoogle Scholar
  34. Brown S (1984) Analysis and sorting of plant material by flow cytometry. Physiol Veg 22: 341–349Google Scholar
  35. Brown S, Renaudin JP, Prevot C, Guern J (1984) Flow cytometry and sorting of plant protoplasts: technical problems and physiological results from a study of pH and alkaloids in Catharanthus roseus. Physiol Veg 22: 541–554Google Scholar
  36. Brown S, Jullien M, Coutos-Thevenat P, Muller Ph, Renaudin JP (1986) Present developments of flow cytometry in plant biology. Biol Cell 58: 173–178Google Scholar
  37. Burgess J (1984) The revolution that failed. New Sci 1429: 26–29Google Scholar
  38. Butenko R, Kuchko A, Komarnitsky I (1982) Some features of somatic hybrids between Solanum tuberosum and S. chacoense and its F1 sexual progeny. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 643–644Google Scholar
  39. Carlsberg I, Glimelius K, Eriksson T (1984) Nuclear DNA content during the initiation of callus for-mation from isolated protoplasts of Solanum tuberosum L. Plant Sci Lett 35: 225–230CrossRefGoogle Scholar
  40. Carlson PS (1973) The use of protoplasts for genetic research. Proc Natl Acad Sci USA 70: 598–602PubMedCrossRefGoogle Scholar
  41. Carlson PS, Smith HH, Dearing RD (1972) Parasexual interspecific plant hybridization. Proc Natl Acad Sci USA 69: 2292–2294PubMedCrossRefGoogle Scholar
  42. Cassells AC, Barlass M (1978) The initiation of TMV infection in isolated protoplasts by polyethylene glycol. Virology 87: 459–462PubMedCrossRefGoogle Scholar
  43. Chilton MD, Drummond MH, Merlo DJ, Sciaky D, Montoya AL, Gordon MP, Nester EW (1977) Stable incorporation of plasmid-DNA into higher plant cells: the molecular basis of crown gall tumorigenesis. Cell 11: 263–271PubMedCrossRefGoogle Scholar
  44. Christou P, Murphy JE, Swain WF (1987) Stable transformation of soybean by electroporation and root formation from transformed callus. Proc Natl Acad Sci USA 84: 3962–3966PubMedCrossRefGoogle Scholar
  45. Chuong PV, Beversdorf WD, Powell AD, Pauls KP (1988) Somatic transfer of cytoplasmic traits in Brassica napus L. by haploid protoplast fusion. Mol Gen Genet 211: 197–201CrossRefGoogle Scholar
  46. Clark E, Schnabelrauch L, Hanson MR, Sink KC (1986) Differential fate of plastid and mitochondria) genomes in Petunia somatic hybrids. Theor Appl Genet 72: 748–755CrossRefGoogle Scholar
  47. Cocking EC, Davey MR (1987) Gene transfer in cereals. Science 236: 1259–1262PubMedCrossRefGoogle Scholar
  48. Cocking EC, Pojnar E (1969) An electron microscopy study of the infection of isolated tomato fruit protoplasts by tobacco mosaic virus. J Gen Virol 4: 305–312CrossRefGoogle Scholar
  49. Conia J, Bergounioux C, Perennes C, Brown S, Muller Ph, Gadal P (1987) Chromosomes from protoplasts of Petunia hybrida, flow analysis and sorting. Biol Cell 58 (Suppl): 5aGoogle Scholar
  50. Constabel F, Kurz WGW, Kutney P (1982) Variation in cell cultures of periwinkle Catharanthus roseus. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 301–304Google Scholar
  51. Crossway A, Hauptli H, Houck CM, Irvine JM, Oakes JV, Perani LA (1986a) Micromanipulation techniques in plant biotechnology. Biotechniques 4: 320–334Google Scholar
  52. Crossway A, Oakes JV, Irvine JM, Ward B, Knauf VC, Showmaker CK (1986b) Integration of foreign DNA following microinjection of tobacco mesophyll protoplasts. Mol Gen Genet 202: 179–185CrossRefGoogle Scholar
  53. Cseplo A, Medgyesy P, Marton L (1986) In vitro induction, isolation and transfer of chloroplast mutations in Nicotiana. Proc Nuclear techniques and in vitro culture for plant improvement. IAEA, Vienna, pp 137–146Google Scholar
  54. Dandekar AK, Gupta PK, Durzan DJ, Knauf V (1987) Transformation and foreign gene expression in micropropagated Douglas-fir (Pseudosuga menziesii). Bio/Technology 5: 587–590CrossRefGoogle Scholar
  55. Davey MR (1977) Bacterial uptake and nitrogen fixation. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 551–562Google Scholar
  56. Davey MR, Cocking EC, Freeman J, Pearce N, Tudor I (1980) Transformation of Petunia protoplasts by isolated Agrobacterium plasmids. Plant Sci Lett 18: 307–313CrossRefGoogle Scholar
  57. De DN, Swain D (1983) Protoplasts, cytoplasts and subprotoplasts from ripening tomato fruits: their nature and fusion properties. In: Sen SK, Giles KL (eds) Plant cell culture in corp improvement. Plenum, New York, pp 201–208Google Scholar
  58. De la Pena A, Lörz H, Schell J (1987) Transgenic rye plants obtained by injecting DNA into young floral tillers. Nature (London) 325: 274–276CrossRefGoogle Scholar
  59. De Laat AMM, Blaas J (1984) Flow cytometric characterization and sorting of plant chromosomes. Theor Appl Genet 67: 463–467CrossRefGoogle Scholar
  60. De Laat AMM, Blaas J (1987) An improved method for protoplast microinjection suitable for transfer of entire plant chromosomes. Plant Sci 50: 161–169CrossRefGoogle Scholar
  61. De Vries SE, Tempelaar MJ (1987) Electrofusion and analysis of potato somatic hybrids. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 3. Potato. Springer, Berlin Heidelberg New York Tokyo, pp 211–222Google Scholar
  62. Deak M, Kiss GB, Koncz C, Dudits D (1986) Transformation of Medicago by Agrobacterium mediated gene transfer. Plant Cell Rep 5: 97–100CrossRefGoogle Scholar
  63. DeLuca M, McElroy WD (1978) Meth Enzymol 57: 3CrossRefGoogle Scholar
  64. D’Hont A, Quetier F, Teoule E, Dattee Y (1987) Mitochondrial and chloroplast DNA analysis of interspecific somatic hybrids of a Leguminoseae: Medicago (alfalfa). Plant Sci 53: 237–242CrossRefGoogle Scholar
  65. Dudits D, Praznonzky T (1985) Intergeneric gene transfer by protoplast fusion and uptake of isolated chromosomes. In: Zaitlin M, Day P, Hollaender A (ed) Biotechnology in plant science: Relevance to agriculture in the eighties. Acad Press, Orlando, pp 115–128Google Scholar
  66. Evans DA, Flick CE, Jensen RA (1981) Incorporation of disease resistance into sexually incompatible somatic hybrids of the genus Nicotiana. Science 213: 907–909PubMedCrossRefGoogle Scholar
  67. Fillatti JJ, Sellmer J, McCown B, Haissig B, Cornai L (1987) Agrobacterium mediated transformation and regeneration of Populus. Mol Gen Genet 206: 192–199Google Scholar
  68. Flavell R, Mathias R (1984) Prospects for transforming monocot crop plants. Nature (London) 307: 108–109CrossRefGoogle Scholar
  69. Fraley RT, Rogers SG, Horsch RB (1986) Genetic transformation in higher plants. CRC Crit Rev Plant Sci 4: 1–46CrossRefGoogle Scholar
  70. Fromm ME, Taylor LP, Walbot V (1985) Expression of genes electroporated into monocot and dicot plant cells. Proc Natl Acad Sci USA 82: 5824–5828PubMedCrossRefGoogle Scholar
  71. Fromm ME, Taylor LP, Walbot V (1986) Stable transformation of maize after gene transfer by electroporation. Nature (London) 319: 791–793CrossRefGoogle Scholar
  72. Galbraith DW, Shields BA (1982) The effect of inhibitors of cell wall synthesis on tobacco protoplast development. Physiol Plant 55: 25–30CrossRefGoogle Scholar
  73. Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051PubMedCrossRefGoogle Scholar
  74. Galbraith DW (1984) Flow cytometric analysis of the cell cycle. In: Vasil IK (ed) Cell culture and somatic cell genetics. Acad Press, Orlando, pp 765–777Google Scholar
  75. Garcia JA, Hille J, Vos P, Goldbach R (1987) Transformation of cowpea Vigna unguiculata with a full-length DNA copy of cowpea mosaic virus m-RNA. Plant Sci 48: 89–98CrossRefGoogle Scholar
  76. Gheysen G, Dhaese P, Van Montague M, Schell J (1985) DNA flux across genetic barriers: The crown gall phenomenon. In: Hohn B, Dennis ES (eds) Genetic flux in plants. Springer, Berlin Heidelberg New York Tokyo, pp 12–47Google Scholar
  77. Giles KL (1973) Complementation by protoplast fusion using mutant strain of maize. Plant Cell Physiol 13: 207–210Google Scholar
  78. Giles KL, Sarafis V (1971) On the survival and reproduction of chloroplasts outside the cell. Cytobios 4: 61–74Google Scholar
  79. Giles KL, Sarafis V (1972) Chloroplast survival and division in vitro. Nature New Biol 236: 56–58PubMedGoogle Scholar
  80. Gleba YY, Hoffmann F (1980) “Arabidobrassica”: A novel plant obtained by protoplast fusion. Planta 149: 112–117CrossRefGoogle Scholar
  81. Gleba YY, Sytnik KM (1984) Protoplast fusion, genetic engineering in higher plants. Springer, Berlin Heidelberg New York, pp 27–28Google Scholar
  82. Gleba YY, Kolesnik NN, Meshkene IV, Cherep NN, Parokonny AS (1984) Transmission genetics of the somatic hybridization process in Nicotiana I. Hybrid and cybrids among the regenerates from cloned protoplast fusion products. Theor Appl Genet 69: 121–128Google Scholar
  83. Gleddie S, Keller WA, Setterfield G (1986) Production and characterization of somatic hybrids between Solanum melongena L. and Solanum sisymbrifolium Lam. Theor Appl Genet 71: 613–621CrossRefGoogle Scholar
  84. Glimelius K (1987) Enrichment of somatic hybrids with flow cytometry. IAPTC Newslett 52: 2–9Google Scholar
  85. Glimelius K, Chen K, Bonnett HT (1981) Somatic hybridization in Nicotiana: Segregation of organellar traits among hybrid and cybrid progeny. Planta 153: 504–510CrossRefGoogle Scholar
  86. Glimelius K, Djupsjobacka M, Fellner-Feldegg H (1986) Selection and enrichment of plant protoplasts heterokaryons of Brassicaceae by flow sorting. Plant Sci 45: 133–141CrossRefGoogle Scholar
  87. Goodman RM, Hauptli H, Crossway A, Knauf CV (1987) Gene transfer in crop improvement. Science 236: 48–54PubMedCrossRefGoogle Scholar
  88. Gould JH, Palmer RL, Dugger WM (1986) Isolation and culture of anucleate protoplasts from cotton (Gossypium hirsutum) fiber: assessment of viability and analysis of regenerated wall polymers. Plant Cell Tissue Organ Cult 6: 61–72CrossRefGoogle Scholar
  89. Griesbach RJ, Sink KC (1983) Evacuolation of mesophyll protoplasts. Plant Sci Lett 30: 297–301CrossRefGoogle Scholar
  90. Grimsley N, Hohn B, Hohn T, Walden R (1986) Agroinfection, a novel route for plant viral infection. Proc Natl Acad Sci USAGoogle Scholar
  91. Grosser JW, Gmitter FG, Chandler JL (1988a) Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha. Theor Appl Genet 75: 397–401CrossRefGoogle Scholar
  92. Grosser JW, Gmitter FG, Chandler JL (1988a) Intergeneric somatic hybrid plants from sexually incompatible woody species: Citrus sinensis and Severinia disticha. Theor Appl Genet 75: 397–401CrossRefGoogle Scholar
  93. Grosser JW, Gmitter FG, Chandler JL (1988b) Intergeneric somatic hybrid plants of Citrus sinensis cv. Hamlin and Poncirus trifoliata cv. Plant Cell Rep 7: 5–8CrossRefGoogle Scholar
  94. Guerche P, Jouanin L, Tepfer D, Pelletier G (1987) Genetic transformation of oilseed rape (Brassica napus) by the Ri T-DNA of Agrobacterium rhizogenes and analysis of inheritance of the transformed phenotype. Mol Gen Genet 206: 382–386CrossRefGoogle Scholar
  95. Hain R, Stabel P, Czernilofski AP, Steinbiss HH, Herrera-Estrella L, Schell J (1985) Uptake, integration and genetic transmission of a selectable chimeric gene by plant protoplasts. Mol Gen Genet 199: 161–168CrossRefGoogle Scholar
  96. Handley LW, Nickels RS, Cameron MW, Moore PP, Sink KC (1986) Somatic hybrid plants between Lycopersicon esculentum and Solanum lycopersicoides. Theor Appl Genet 71: 691–697CrossRefGoogle Scholar
  97. Hepher A, Sherman A, Gates P, Boutler D (1985) Microinjection of gene vectors into pollen and ovaries as a potential means of transforming whole plants. In: Chapman GP, Mantell SH, Daniels RW (eds) The experimental manipulation of ovule tissues. Longman, New York, pp 52–63Google Scholar
  98. Herrera-Estrella L, Broeck G van den, Maenhaust R, Van Montagu M, Schell J, Timko M, Cashmore A (1984) Light-inducible and chloroplast associated expression of a chimaeric gene introduced into Nicotiana tabacum using a Ti plasmid vector. Nature (London) 310: 115–120CrossRefGoogle Scholar
  99. Herzenberg LA, Sweet RG (1976) Fluorescence-activated cell sorting. Sci Am 234: 108–117PubMedCrossRefGoogle Scholar
  100. Horsch RB, Fry JB, Hoffmann NL, Wallroth M, Eichholtz D, Rogers SG, Fragley RT (1985) A simple and general method for transferring genes into plants. Science 227: 1229–1231CrossRefGoogle Scholar
  101. Hurlbut P, Fellner-Feldegg H, Glimelius K (1985) Sorting of miniprotoplasts by flow cytometry. Hereditas Suppl 3: 142Google Scholar
  102. Ichikawa H, Tanno-Suenaga L, Imamura J (1987) Selection of Daucus cybrids based on metabolic complementation between X-irradiated D. capillifolius and iodoacetamide-treated D. carota by somatic cell fusion. Theor Appl Genet 74: 746–752CrossRefGoogle Scholar
  103. Ito K, Tatemichi Y, Shibata M (1986) Regeneration of poplar-like plants from fusion between Populus (hybrid) and Hibiscus. Proc VI Int Congr Plant tissue and cell culture, Univ Minnesota, MN, Abstr, p 389Google Scholar
  104. Jaenisch R, Mintz B (1974) Simian virus 40 DNA sequences in DNA of healthy adult mice derived from preimplantation blastocysts injected with viral DNA. Proc Natl Acad Sci USA 71: 1250–1254PubMedCrossRefGoogle Scholar
  105. Jordan MC, McHughen A (1988) Glyphosate-tolerant flax plants from Agrobacterium mediated gene transfer. Plant Cell Rep 7: 281–284CrossRefGoogle Scholar
  106. Kamada H, Okamura N, Satake M, Harada H, Shimomura K (1986) Alkaloid production by hairy root cultures in Atropa belladonna. Plant Cell Rep 5: 239–242CrossRefGoogle Scholar
  107. Kemble RJ, Barsby TL, Wong RSC, Shepard JF (1986) Mitochondria] DNA rearrangements in somatic hybrids of Solanum tuberosum and Solanum brevidens. Theor Appl Genet 72: 787–793CrossRefGoogle Scholar
  108. Klee H (1987) Agrobacterium-mediated plant transformation and its further applications to plant biology. Annu Rev Plant Physiol 38: 467–486CrossRefGoogle Scholar
  109. Kohler F, Golz C, Eapen S, Kohn H, Schieder 0 (1987) Stable transformation of moth bean Vigna acontifolium via direct gene transfer. Plant Cell Rep 6: 313–317Google Scholar
  110. Krens FA, Molendijk L, Wullems GJ, Schilperoort RA (1982) In vitro transformation of plant protoplasts with Ti-plasmid DNA. Nature (London) 296: 72–74CrossRefGoogle Scholar
  111. Kubo T (1985) Bull IWATA Tobacco. IWATA Exp Stn 17: 130–133Google Scholar
  112. Kumar A, Cocking EC (1987) Protoplast fusion: a novel approach to organelle genetics in higher plants. Am J Bot 74: 1289–1303CrossRefGoogle Scholar
  113. Langridge WHR, Li BJ, Szalay AA (1985) Electric field mediated stable transformation of carrot protoplasts with naked DNA. Plant Cell Rep 4: 355–359CrossRefGoogle Scholar
  114. Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214CrossRefGoogle Scholar
  115. Lesney MS, Callow PW, Sink KC (1986) A technique for bulk production of cytoplasts and miniprotoplasts from suspension culture-derived protoplasts. Plant Cell Rep 5: 115–118CrossRefGoogle Scholar
  116. Levings CS, Kim BD, Pring DR, Conde MF, Mans RJ (1980) Cytoplasmic reversion of cms-S, in maize: association with a transpositional event. Science 209: 345–347CrossRefGoogle Scholar
  117. Lonsdale DM (1987) Cytoplasmic male sterility: a molecular perspective. Plant Physiol Biochem 25: 265–271Google Scholar
  118. Lörz H (1984) Enucleation of protoplasts: Preparation of cytoplasts and miniprotoplasts. In: Vasil IK (ed) Cell culture and somatic cell genetics of plants, vol 1. Academic Press, New York London, pp 448–453Google Scholar
  119. Lörz H, Potrykus I (1978) Investigations on the transfer of isolated nuclei into plant protoplasts. Theor Appl Genet 53: 251–256Google Scholar
  120. Lörz H, Potrykus I (1980) Isolation of subprotoplasts for genetic manipulation studies. In: Ferenczy L, Farkas GL, Lazar G (eds) Advances in protoplast research. Pergamon, Oxford, pp 377–382Google Scholar
  121. Lörz H, Paszkowski J, Dierks-Ventling C, Potrykus I (1981) Isolation and characterization of cytoplasts and miniprotoplasts derived from protoplasts of cultured cells. Physiol Plant 53: 385–391CrossRefGoogle Scholar
  122. Lörz H, Baker B, Schell J (1985) Gene transfer to cereal cells mediated by protoplast transformation. Mol Gen Genet 199: 178–182CrossRefGoogle Scholar
  123. Lurquin PF (1984) Liposomes as carriers for the transfer of nucleic acids into higher plant protoplasts. In: Beers RF, Bassett EG, Jr (eds) Cell fusion, gene transfer and transformation. Raven, New York, pp 227–236Google Scholar
  124. Lynch PT, Isaac S, Collin HA (1986) Uptake of fungal protoplasts by plant protoplasts. Abstr VIth Int Congr Plant tissue and cell culture, Univ Minnesota, p 390Google Scholar
  125. Maliga P, Lörz H, Lazar G, Nagy F (1982) Cytoplast-protoplast fusion for interspecific chloroplast transfer in Nicotiana. Mol Gen Genet 185: 211–215CrossRefGoogle Scholar
  126. Manners JM (1987) Transformation of Stylosanthes spp. using Agrobacterium tumefaciens. Plant Cell Rep 6: 204–207CrossRefGoogle Scholar
  127. Mansfield JW, Hargreaves JA, Boyle FC (1974) Phytoalexin production by live cells in broad bean leaves infected with Botrytis cinerea. Nature (London) 252: 316–317CrossRefGoogle Scholar
  128. Matthews BF, Widholm JM (1986) Organelle DNA compositions and isoenzyme expression in an interspecific somatic hybrid of Daucus. Mol Gen Genet 198: 371–376CrossRefGoogle Scholar
  129. McCormick S, Niedermeyer J, Fry J, Banason A, Horsch R, Fraley R (1986) Leaf disc transformation of cultivated tomato (Lycopersicon esculentum) using Agrobacterium tumefaciens. Plant Cell Rep 5: 81–86CrossRefGoogle Scholar
  130. Meadows MR (1982) Characterization of cells and protoplasts of the B73 maize cell line. Plant Sci Lett 28: 337–348Google Scholar
  131. Medgyesy P, Golling R, Nagy F (1985) A light sensitive recipient for the effective transfer of chloroplast and mitochondrial traits by protoplast fusion in Nicotiana. Theor Appl Genet 70: 590–594CrossRefGoogle Scholar
  132. Melamed MR, Mullaney PF, Mendelsohn ML (1979) Flow cytometry and sorting. J. Wiley Sons, New YorkGoogle Scholar
  133. Melchers G, Sacristan MD, Holder AA (1978) Somatic hybrid plants of potato and tomato regenerated from fused protoplasts. Carlsberg Res Commun 43: 203–218CrossRefGoogle Scholar
  134. Menczel L, Galiba G, Nagy F, Maliga P (1982) Effect of radiation dosage on efficiency of chloroplast transfer by protoplast fusion in Nicotiana. Genetics 100: 43–52Google Scholar
  135. Miki BL, Reich TJ, Iyer VN (1986) Microinjection technology for cultured plant cells. Proc VI Int Congr Plant tissue and cell culture, Univ Minnesota, p 313Google Scholar
  136. Nagata T (1984) Interaction of liposomes and protoplasts as a model system of protoplasts fusion. In: Beers RF Jr, Bassett EG (eds) Cell fusion, gene transfer and transformation. Raven, New York, pp 217–226Google Scholar
  137. Nass MMK (1969) Uptake of isolated chloroplasts by mammalian cells. Science 165: 1128–1131PubMedCrossRefGoogle Scholar
  138. Negrutiu I, Shillito R, Potrykus I, Biasini G, Sala F (1987) Hybrid genes in the analysis of transformation conditions,I, Setting up a simple method for direct gene transfer in plant protoplasts. Plant Mol Biol 8: 363–373CrossRefGoogle Scholar
  139. Neuhaus G, Spangenberg G, Millelsten Sceid O, Schweiger HG (1987) Transgenic rapeseed plants obtained by the microinjection of DNA into microspore derived embryoids. Theor Appl Genet 75: 30–36CrossRefGoogle Scholar
  140. Neumann E, Schaefer-Ridder M, Wang Y, Hofschneider PH (1982) Gene transfer into mouse lyoma cells by electroporation in high electric fields. EMBO J 1: 841–845PubMedGoogle Scholar
  141. Nomura K, Komamine A (1986) Embryogenesis from microinjected single cells in a carrot cell suspension culture. Plant Sci 44: 53–58CrossRefGoogle Scholar
  142. O’Connell MA, Hanson MR (1986) Regeneration of somatic hybrid plants formed between Lycopersicon esculentum and Solanum rickii. Theor Appl Genet 72: 243–249Google Scholar
  143. Ohgawara D, Kobayashi S, Oghawara E, Uchimiya H, Ishii S (1985) Somatic hybrid plants obtained by protoplast fusion between Citrus sinensis and Poncirus trifoliata. Theor Appl Genet 71: 1–4CrossRefGoogle Scholar
  144. Okada K, Nagata T, Takebe I (1986) Introduction of functional RNA into plant protoplasts by electroporation. Plant Cell Physiol 27: 619–626Google Scholar
  145. Ooms G, Bossen ME, Burrell M, Karp A (1986) Genetic manipulation of potato with Agrobacterium rhizogenes. Potato Res 29: 367–379CrossRefGoogle Scholar
  146. Otsuki Y, Takebe I, Honda Y, Matsui C (1972) Ultrastructure of infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Virology 49: 188–194PubMedCrossRefGoogle Scholar
  147. Ou-Lee TM, Turgeon R, Wu R (1986) Expression of a foreign gene linked to either a plant-virus or a Drosophilla promoter, after electroporation of protoplasts of rice, wheat and sorghum. Proc Natl Acad Sci USA 83: 6815–6819PubMedCrossRefGoogle Scholar
  148. Ow DW, Wood KV, DeLuca M, de Wet JR, Helinski DR, Howell SH (1986) Transient and stable ex- pression of the firefly luciferase gene in plant cells and transgenic plants. Science 234: 856–859PubMedCrossRefGoogle Scholar
  149. Pasternak TP, Melnikov PV, Gleba YY, Sytnik KM, Argentova VV, Kaliev AB, Andrianov VM, Piruzyan ES (1986) Genetic transformation of higher plant cells by DNA microinjections. Proc USSR Acad Sci Ser Biol 2: 316Google Scholar
  150. Paszkowski J, Shillito RD, Mandak SM, Hohn T, Hohn B, Potrykus I (1984) Direct gene transfer to plants. EMBO J 3: 2717–2722PubMedGoogle Scholar
  151. Payne J, Hamill JD, Robins RJ, Rhodes MJC (1987) Production of hyoscyamine by `hairy root’ cultures in Datura stramonium. Planta Med 53: 474–478PubMedCrossRefGoogle Scholar
  152. Payne J, Hamill JD, Robins RJ, Rhodes MJC (1987) Production of hyoscyamine by `hairy root’ cultures in Datura stramonium. Planta Med 53: 474–478PubMedCrossRefGoogle Scholar
  153. Pelletier C, Primand C, Vedel F, Chetrie P, Remy R, Rousselle P, Renard M (1983) Intergeneric cytoplasmic hybridization in Cruciferae by protoplast fusion. Mol Gen Genet 191: 244–250CrossRefGoogle Scholar
  154. Perani L, Radke S, Wilke-Douglas M, Bossert M (1986) Gene transfer methods for crop improvement: Introduction of foreign DNA into plants. Physiol Plant 68: 566–570CrossRefGoogle Scholar
  155. Petit P (1986) Flow cytofluorometry analysis of isolated plant and animal mitochondria. Biol Cell 58 (Suppl): 15aGoogle Scholar
  156. Pinkel D (1981) On the possibility of automated scoring of pollen mutants. Environ Health Perspect 37: 133–136PubMedCrossRefGoogle Scholar
  157. Pirrie A, Power JB (1986) The production of fertile, haploid somatic hybrid plant (Nicotiana glutinosa (N)+N. tabacum (2N) via gametic: somatic protoplast fusion. Theor Appl Genet 72: 48–52CrossRefGoogle Scholar
  158. Potrykus I (1973) Transplantation of chloroplasts into protoplasts of Petunia. Z Pflanzenphysiol 70: 364–366Google Scholar
  159. Potrykus I, Hoffmann F (1973) Transplantations of nuclei into protoplasts of higher plants. Z Pflanzenphysiol 69: 287–289Google Scholar
  160. Potrykus I, Shillito RD, Saul MW, Paszkowski J (1985a) Direct gene transfer; State of the art and future potential. Plant Mol Biol Rep 3: 117–128CrossRefGoogle Scholar
  161. Potrykus I, Saul MW, Petruska J, Paszkowski J, Shillito RD (1985b) Direct gene transfer to cells of a graminaceous monocot. Mol Gen Genet 199: 183–188CrossRefGoogle Scholar
  162. Puite KJ, Roest S, Pijnacker LP (1986) Somatic hybrid plants after electrofusion of diploid Solanum tuberosum and S. phureja. Plant Cell Rep 5: 262–265CrossRefGoogle Scholar
  163. Reich TJ, Iyer VN, Haffner M, Holbrook LA, Miki BL (1986a) The use of fluorescent dyes in the microinjection of alfalfa protoplasts. Can J Bot 64: 1259–1267CrossRefGoogle Scholar
  164. Reich TJ, Iyer VN, Scobie B, Miki BL (1986b) A detailed procedure for the intranuclear microinjection of plant protoplasts. Can J Bot 64: 1255–1258CrossRefGoogle Scholar
  165. Reich TJ, Iyer VN, Miki BL (1986c) Efficient transformation of alfalfa protoplasts by the intranuclear microinjection of Ti plasmids. Bio/Technology 4: 1001–1003CrossRefGoogle Scholar
  166. Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JL (1988) Genetically transformed maize plants from protoplasts. Science 240: 204–207PubMedCrossRefGoogle Scholar
  167. Rhodes MJC, Robins RJ, Hamill JD, Parr AJ, Walton NJ (1987) Secondary product formation using Agrobacterium rhizogenes-transformed “hairy root” cultures. IAPTC Newslett 53: 2–15Google Scholar
  168. Riggs CD, Bates GW (1986) Stable transformation of tobacco by electroporation: Evidence for plasmid concentration. Proc Natl Acad Sci USA 83: 5602–5606PubMedCrossRefGoogle Scholar
  169. Robertson D, Palmer JD, Earle ED, Mutschler MA (1987) Analysis of organelle genomes in a somatic hybrid derived from cytoplasmic male-sterile Brassica oleracea and atrazine-resistant B. campestris. Theor Appl Genet 74: 303–309CrossRefGoogle Scholar
  170. Rohme D, Fox H, Herrmann B, Frischauf AM, Edstrom JE, Mains P, Silver LM, Lehrach H (1984) Molecular clones of the mouse complex derived from microdissected metaphase chromosomes. Cell 35: 783–788CrossRefGoogle Scholar
  171. Saxena PK, Fowke LC, King J (1985a) An efficient procedure for isolation of nuclei from plant protoplasts. Protoplasma 128: 184–189CrossRefGoogle Scholar
  172. Saxena PK, Liu Y, Mii M, Fowke LC, King J (1985b) High nuclear yield from protoplasts of several plants. J Plant Physiol 121: 193–197Google Scholar
  173. Saxena PK, Mii M, Crosby WL, Fowke LC, King J (1986) Transplantation of isolated nuclei into plant protoplasts. Planta 168: 29–35CrossRefGoogle Scholar
  174. Saxena PK, Liu Y, King J (1987) Nuclear transplantation into protoplasts: Optimal conditions for induction and determination of nuclear uptake. J Plant Physiol 128: 451–460Google Scholar
  175. Schell J St (1987) Transgenic plants as tools to study the molecular organization of plant genes. Science 237: 1176–1183CrossRefGoogle Scholar
  176. Scheurich P, Zimmermann U, Schnabl H (1981) Electrically stimulated fusion of different plant cell protoplasts. Plant Physiol 67: 849–853PubMedCrossRefGoogle Scholar
  177. Sederoff R, Stomp AM, Chilton WS, Moore LW (1986) Gene transfer into Loblolly pine by Agrobacterium tumefaciens. Bio/Technology 4: 647–649CrossRefGoogle Scholar
  178. Shah DM, Horsch RB, Klee HJ, Kishore GM, Winter JA, Turner NE, Hironaka CM, Sanders PR, Gasser CS, Aykent S, Siegel NR, Rogers SG, Fraley RT (1986) Engineering herbicide tolerance in transgenic plants. Science 233: 478–481PubMedCrossRefGoogle Scholar
  179. Shahin EA, Sukhapinda K, Simpson RB, Spivey R (1986 a) Transformation of cultivated tomato by a binary vector in Agrobacterium rhizogenes: transgenic plants with normal phenotypes harbor binary vector T-DNA, but no Ri-plasmid T-DNA. Theor Appl Genet 72: 770–777Google Scholar
  180. Shahin EA, Spielmann A, Sukhapinda K, Simpson RB, Yashar M (1986b) Transformation of cultivated alfalfa using disarmed Agrobacterium tumefaciens. Crop Sci 26: 1235–1239CrossRefGoogle Scholar
  181. Shillito RD, Saul MW, Paszkowski J, Muller M, Potrykus I (1985) High efficiency direct gene transfer to plants. Bio/Technology 3: 1099–1103CrossRefGoogle Scholar
  182. Shillito RD, Saul MW, Paszkowski J, Potrykus I (1986) Direct gene transfer to plants. IAPTC Newslett 48: 5–15Google Scholar
  183. Shvidkaya LG, Gleba YY (1974) Plating mesophyll protoplasts, isolated from different photosynthetic mutants of Nicotiana tabacum L. 3rd Int Congr Plant cell tissue culture, Abstr 195, Leicester UnivGoogle Scholar
  184. Spangenberg G, Schweiger H-G (1986) Controlled electrofusion of different types of protoplasts and subprotoplasts including cell reconstitution in Brassica napus L. Eur J Cell Biol 41: 51–56Google Scholar
  185. Sree Ramulu K, Verhoeven HA, Gilissen LJW, Dijkhuis P, van der Valk HCPM (1987) Induction of micronuclei and their application for transfer of single, specific chromosomes in plant cells. Abstr 7th Int Protoplast Symp, Wageningen, p 33Google Scholar
  186. Steinbiss HH, Stabel P (1983) Protoplast derived tobacco cells can survive capillary microinjection of the fluorescent dye Lucifer yellow. Protoplasma 116: 223–227CrossRefGoogle Scholar
  187. Sundberg E, Glimelius K (1986) A method for production of interspecific hybrids within Brassiceae via somatic hybridization, using resynthesis of Brassica napus as a model. Plant Sci 43: 155–162CrossRefGoogle Scholar
  188. Szabados L, Hadlaczky GY, Dudits D (1981) Uptake of isolated plant chromosomes by plant protoplasts. Planta 151: 141–145CrossRefGoogle Scholar
  189. Tagu D, Bergounioux C, Perennes C, Brown S, Muller P, Gadal P (1987) Analysis by flow cytometry of the primary events of Petunia hybrida protoplast transformation. Plant Sci 51: 215–223CrossRefGoogle Scholar
  190. Takebe I (1983) Protoplasts in plant virus research. Int Rev Cytol Suppl 16: 89–111Google Scholar
  191. Tan M-L MC (1987) Somatic hybridization and cybridization in Solanaceous species. Academisch Proefschrift. Vrije Univ te AmsterdamGoogle Scholar
  192. Tao W, Wilkinson J, Stanbridge EJ, Berns MW (1987) Direct gene transfer into human cultured cells facilitated by laser micropuncture of the cell membrane. Proc Natl Acad Sci USA 84: 4180–4184PubMedCrossRefGoogle Scholar
  193. Tempelaar MJ, Jones MGK (1985) Fusion characteristics of plant protoplasts in electric fields. Planta 165: 205–216CrossRefGoogle Scholar
  194. Terada R, Kyozuka J, Nishibayashi S, Shimamoto K (1987) Plantlet regeneration from somatic hybrids of rice (Oryza sativa L.) and barnyard grass (Echinochloa oryzicola Vasing). Mol Gen Genet 210: 39–43CrossRefGoogle Scholar
  195. Toriyama K, Hinata K, Kameya T (1987a) Production of somatic hybrid plants ‘Brassicamoricandia’, through protoplast fusion between Moricandia arvensis and Brassica oleracea. Plant Sci 48: 123–128CrossRefGoogle Scholar
  196. Toriyama K, Kameya T, Hinata K (1987b) Selection of a universal hybridizer in Sinapis turgida Del. and regeneration of plantlets from somatic hybrids with Brassica species. Planta 170: 308–313CrossRefGoogle Scholar
  197. Trulson AJ, Simpson RB, Shahin EA (1986) Transformation of cucumber (Cucumis sativus L.) plants with Agrobacterium rhizogenes. Theor Appl Genet 73: 11–15CrossRefGoogle Scholar
  198. Uchimiya H, Fushimi T, Hashimoto H, Harada H, Syono K, Sugawara Y (1986) Expression of a foreign gene in callus derived from DNA-treated protoplasts of rice (Oryza sativa L.). Mol Gen Genet 204: 204–207CrossRefGoogle Scholar
  199. Vardi A, Breiman A, Galun E (1987) Citrus cybrids: Production by donor recipient protoplast-fusion and verification by mitochondrial-DNA restriction profiles. Theor Appl Genet 75: 51–58CrossRefGoogle Scholar
  200. Vlachova M, Metz BA, Schell J, de Bruijn FJ (1987) The tropical legume Sesbania rostrata: tissue culture, plant regeneration and infection with Agrobacterium tumefaciens and rhizogenes strains. Plant Sci 50: 213–223CrossRefGoogle Scholar
  201. Wallin A, Glimelius K, Eriksson T (1978) Enucleation of plant protoplasts by cytochalasin B. Z Pflanzenphysiol 87: 330–340Google Scholar
  202. Wallin A, Glimelius K, Eriksson T (1979) Formation of hybrid cells by transfer of nuclei via fusion of miniprotoplasts from cell lines of nitrate reductase deficient tobacco. Z Pflanzenphysiol 91: 89–94Google Scholar
  203. Watts JW, King JM (1984) A simple method for large scale electrofusion and culture of plant protoplasts. Biosci Rep 4: 335–342PubMedCrossRefGoogle Scholar
  204. Watts JW, King JM, Stacey NJ (1987) Inoculation of protoplasts with viruses by electroporation. Virology 157: 40–46PubMedCrossRefGoogle Scholar
  205. Webb KJ (1986) Transformation of forage legumes using Agrobacterium tumefaciens. Theor Appl Genet 72: 53–58CrossRefGoogle Scholar
  206. Wenzel G, Schieder O, Przewozny T, Sopory SK, Melchers G (1979) Comparison of single cell culture-derived Solanum tuberosum L. plants and model for their application in breeding programs. Theor Appl Genet 55: 49–55CrossRefGoogle Scholar
  207. White DWR, Greenwood D (1987) Transformation of the forage legume Trifolium repens L. using binary Agrobacterium vectors. Plant Mol Biol 8: 461–469CrossRefGoogle Scholar
  208. Wilke-Douglas M, Perani L, Radke S, Bossert M (1986) The application of recombinant DNA technology toward crop improvement. Physiol Plant 68: 560–565CrossRefGoogle Scholar
  209. Wright RL, Somers DA, McGraw RL (1986) Somatic hybridization between birdsfoot trefoil (Lotus corniculatus) and L. coimbrensis. Proc Vlth Int Congr Plant tissue and cell culture, Univ Minnesota, p 86Google Scholar
  210. Wullems GJ, Molendijk L, Ooms G, Schilperoort RA (1981) Differential expression of crown gall tumor markers in transformants obtained after in vitro Agrobacterium tumefaciens-induced transformation of cell wall regenerating protoplasts derived from Nicotiana tabacum. Proc Natl Acad Sci USA 78: 4344–4348PubMedCrossRefGoogle Scholar
  211. Zachrisson A, Bornman CH (1986) Electromanipulation of plant protoplasts. Physiol Plant 67: 507–516CrossRefGoogle Scholar
  212. Zaenen I, van Lerebeke N, Teuchy H, Montague M van, Schell J (1974) Supercoiled circular DNA in crown gall-inducing Agrobacterium strains. J Mol Biol 86: 109–127PubMedCrossRefGoogle Scholar
  213. Zambryski P, Herrera-Estrella L, De Block M, Montague M van, Schell J (1984) The use of the Ti plasmid of Agrobacterium to study the transfer and expression of foreign DNA in plant cells: new vectors and methods. In: Setlow J, Hollaender A (eds) Genetic engineering, principles and methods. Plenum, New York, pp 253–278Google Scholar
  214. Zelcer A, Aviv D, Galun E (1978) Interspecific transfer to cytoplasmic male sterility by fusion between protoplast of normal Nicotiana sylvestris and X-ray irradiated protoplasts of male sterile N. tabacum. Z Pflanzenphysiol 90: 397–407Google Scholar
  215. Zimmermann U, Scheurich P (1981) High frequency fusion of plant protoplasts by electric fields. Planta 151: 26–32CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Y. P. S. Bajaj
    • 1
  1. 1.Punjab Agricultural UniversityLudhianaIndia

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