Genetic Engineering and In Vitro Manipulation of Plant Cells — Technical Advances

  • Y. P. S. Bajaj
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 9)


It is envisaged that the next agricultural revolution will be through in vitro technology. Tremendous progress made during the last decade has amply demonstrated that refinement of routine in vitro culture techniques and development of new biotechnologies such as somatic cell hybridization, transformation through Agrobacterium, direct gene transfer, electrofusion, electroporation, flow cytometry, uptake of nuclei, chromosomes, DNA, chloroplasts, bacteria, viruses, fungi, and the transfer of mitochondria by protoplasts and other recombinant DNA techniques have enabled the opening of new vistas for plant improvement (Potrykus et al. 1985 a; Bajaj 1986, 1989; Crossway et al. 1986a; Perani et al. 1986; Cocking and Davey 1987; Schell 1987). Protoplasts have been shown to be excellent tools for genetic manipulation, since entire plants can be regenerated from them under suitable culture conditions — a prerequisite for genetic manipulation studies. The subject of isolation and culture of protoplasts has already been dealt with in detail and now there are a large number of species in which entire plants can be routinely regenerated (see Bajaj 1989).


Cytoplasmic Male Sterility Somatic Hybrid Protoplast Fusion Agrobacterium Rhizogenes Petunia Hybrida 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • Y. P. S. Bajaj
    • 1
  1. 1.Punjab Agricultural UniversityLudhianaIndia

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