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Soybean: Plant Regeneration and Somaclonal Variation

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Legumes and Oilseed Crops I

Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 10))

Abstract

The soybean, a legume of Eastern Asian origin, is a major source of edible vegetable oils and of high protein feed supplements for livestock. The demand for soybean, being the most produced, traded, and utilized meal and oil in the world, has resulted in increased soybean production, and research on this crop has also increased proportionally (Smith and Huyser 1987).

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References

  • Alexander DE, Silvera L, Collins FI, Rodgers RC (1967) Analysis of oil content of maize by wide-line NMR. J Am Oil Chem Soc 44: 555–558

    Article  PubMed  CAS  Google Scholar 

  • Allington WB, Chamberlain DW (1948) Brown stem rot of soybean. Phytopathol 38: 793–802

    Google Scholar 

  • Baley JM, King J, Gamborg OL (1972 a) The effect of the source of inorganic nitrogen on growth and enzymes of nitrogen assimilation in soybean and wheat cells in suspension cultures. Planta 105: 15–24

    Google Scholar 

  • Baley JM, King J, Gamborg OL (1972 b) The ability of amino compounds and conditioned medium to alleviate the reduced nitrogen requirement of soybean cells grown in suspension cultures. Planta 105: 25–32

    Google Scholar 

  • Barwale UB (1987) Somaclonal variation in soybeans. Ph D Thesis, Univ Illinois Urbana-Champaign

    Google Scholar 

  • Barwale UB, Widholm JM (1987) Somaclonal variation in plants regenerated from cultures of soybean. Plant Cell Rep 6: 365–368

    Article  Google Scholar 

  • Barwale UB, Meyer MM, Widholm JM ( 1986 a) Screening of Glycine max (L.)Merr. and G. soja Sieb. and Zucc. genotypes for multiple shoot formation at the cotyledonary node. Theor Appl Genet 72: 423–428

    Google Scholar 

  • Barwale UB, Kerns HA, Widholm JM (1986 b) Plant regeneration from callus cultures of several soybean genotypes via embryogenesis and organogenesis. Planta 167: 473–480

    Google Scholar 

  • Beversdorf WD, Bingham ET (1977) Degrees of differentiation obtained in tissue cultures of Glycine species. Crop Sci 17: 307–311

    Article  CAS  Google Scholar 

  • Bingham ET, McCoy TJ (1986) Somaclonal variation in alfalfa. Plant Breed Rev 4: 123–152

    Google Scholar 

  • Capelle SC, Mok DWS, Kirchner SC, Mok MC (1983) Effects of thidiazuron on cytokinin autonomy and the metabolism of N6-(delta2-isopentyl) [8-14C] adenosine in callus tissues of Phaseolus lunatus L. Plant Physiol 73: 796–802

    Article  PubMed  CAS  Google Scholar 

  • Cheng TY, Saka H, Voqui-Dinh TH (1980) Plant regeneration from soybean cotyledonary node segments in culture. Plant Sci Lett 19: 91–99

    Article  CAS  Google Scholar 

  • Christianson ML, Warnick DA, Carlson PS (1983) A morphogenetically competent soybean suspension culture. Science 222: 632–634

    Article  PubMed  CAS  Google Scholar 

  • Cowan JC (1973) Processing and products. In: Caldwell BE (ed) Soybeans: improvement, production, and uses. Am Soc Agron Madison, WI, pp 619–664

    Google Scholar 

  • D’Amato F, Bennici A, Cionini PG, Baroncelli S, Lupi MC (1980) Nuclear fragmentation followed by mitosis as mechanisms for wide chromosome number variation in tissue culture: its implications for plantlet regeneration. In: Sala F, Parisi R, Cella R, Ciferri D (eds) Plant cell cultures: results and perspective. Elsevier Amsterdam, pp 67–72

    Google Scholar 

  • Gamborg OL, Shyluk JP (1970) The culture of plant cells with ammonium salts as the sole nitrogen source. Plant Physiol 45: 598–600

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Miller RA, Ojima K (1968) Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50: 151–158

    Article  PubMed  CAS  Google Scholar 

  • Gamborg OL, Davies BP, Stahlhut RW (1983) Cell division and differentiation in protoplasts from cell cultures of Glycine species and leaf tissue of soybean. Plant Cell Rep 2: 213–215

    Article  Google Scholar 

  • Ghazi TD, Cheema HV, Nabors MW (1986) Somatic embryogenesis and plant regeneration from embryogenic callus of soybean, Glycine max L. Plant Cell Rep 5: 452–456

    Google Scholar 

  • Gonzales RA, Das PK, Widholm JM (1984) Characterization of cultured tobacco cell lines resistant to ethionine, a methionine analog. Plant Physiol 74: 640–644

    Article  PubMed  CAS  Google Scholar 

  • Grant J (1984) Plant regeneration from cotyledonary tissue of Glycine canescens, a perennial wild relative of soybean. Plant Cell Tissue Organ Cult 3: 169–173

    Article  Google Scholar 

  • Gray LE, Chamberlain DW (1975) Evidence for toxin production by a strain of Cephalosporium gregata. Phytopathol 65: 89–90

    Article  Google Scholar 

  • Gray LE, Guan YQ, Widholm JM (1986) Reaction of soybean callus to culture filtrates of Phielophora gregata. Plant Sci 47: 45–55

    Article  Google Scholar 

  • Graybosch RA, Edge ME, Delannay X (1987) Somaclonal variation in soybean plants regenerated from the cotyledonary node tissue culture system. Crop Sci 27: 803–806

    Article  Google Scholar 

  • Guangchu Y, Zhiyin Z, Zhen X, Li C, Xuezhan L, Fengyun B (1984) Studies on the anther culture and its androgenesis in vitro of soybean (Glycine max L. Merr.). Int Symp Genetic Manipulation in Crops. Oct 22–26, Beijing, China

    Google Scholar 

  • Hammatt N, Kim H-I, Davey MR, Nelson RS, Cocking EC (1987) Plant regeneration from cotyledon protoplasts of Glycine canescens and G. clandestina. Plant Sci 48: 129–135

    Article  Google Scholar 

  • Hammond EG, Fehr WR, Synder HE (1972) Improving soybean quality by plant breeding. IBID 49: 33–35

    CAS  Google Scholar 

  • Hildebrandt DF, Phillips GC, Collins GB (1986) Soybean (Glycine max (L.) Merr.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 2. Crops I. Springer, Berlin Heidelberg New York, pp 283–308

    Google Scholar 

  • Hymowitz T, Collins FI, Sedgwick VE, Clark RW (1970) The oil and fatty acid content of some accessions of Glycine wightii. Trop Agric (Trinidad) 48: 265

    Google Scholar 

  • Hymowitz T, Dudley JW, Collins FI, Brown CM (1974) Estimation of protein and oil concentration in corn, soybean and oat seed by near-infrared light reflectance. Crop Sci 14: 713–715

    Article  Google Scholar 

  • Hymowitz T, Chalmers NL, Costanza SH, Saam MM (1986) Plant regeneration from leaf explants of Glycine clandestina Wendl. Plant Cell Rep 3: 192–194

    Article  Google Scholar 

  • Ivers DR, Palmer RG, Fehr WR (1974) Anther culture in soybeans. Crop Sci 14: 891–893

    Article  Google Scholar 

  • Kameya T, Widholm JM (1981) Plant regeneration from hypocotyl sections of Glycine species. Plant Sci Lett 21: 289–294

    Article  CAS  Google Scholar 

  • Kao KN, Keller WA, Miller RA (1970) Cell division in newly formed cells from protoplasts of soybean. Exp Cell Res 62: 338–340

    Article  PubMed  CAS  Google Scholar 

  • Kartha KK, Gamborg OL, Constabel F (1974) Regeneration of pea ( Pisum sativum L.) plants from shoot apical meristems. Z Pflanzenphysiol 72: 172–176

    Google Scholar 

  • Kerns HR, Barwale UB, Meyer MM Jr, Widholm JM (1986) Correlation of cotyledonary node shoot proliferation and somatic embryoid development in suspension cultures of soybean (Glycine max L. Merr. ). Plant Cell Rep 5: 140–143

    Google Scholar 

  • Kimball SL, Bingham ET (1973) Adventitious bud development of soybean hypocotyl sections in culture. Crop Sci 13: 758–760

    Article  Google Scholar 

  • Larkin PJ, Scowcroft WR (1981) Somaclonal variation - A novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60: 197–214

    Article  Google Scholar 

  • Lazzeri PA, Hildebrand DF, Collins GB (1985) A procedure for plant regeneration from immature cotyledon tissue of soybean. Plant Mol Biol Rep 3: 160–167

    Article  Google Scholar 

  • Li BJ, Langridge WHR, Szalay AA (1985) Somatic embryogenesis and plantlet regeneration in soybean Glycine max. Plant Cell Rep 4: 344–347

    Article  CAS  Google Scholar 

  • Lippmann B, Lippmann G (1984) Induction of somatic embryos in cotyledonary tissue of soybean, Glycine max L. Merr. Plant Cell Rep 3: 215–218

    Google Scholar 

  • Lu DY, Cooper-Bland S, Pental D, Cocking EC, Davey MR (1983) Isolation and sustained division of protoplasts from cotyledons of seedlings and immature seeds of Glycine max L. Z Pflanzenphysiol 111: 389–394

    CAS  Google Scholar 

  • Mathews VHM, Rao PS, Bhatia CR (1986) Somaclonal variation in cotyledonary plants of mung bean. Z Pflanzenzucht 96: 169–173

    Google Scholar 

  • Mok MC, Mok DWS, Armstrong DJ, Shudo K, Isogai Y, Okamato T (1982) Cytokinin activity of N-phenyl-N’-1,2,3-thiadiazol-5-ylurea ( Thidiazuron ). Phytochem 21: 1509–1511

    Google Scholar 

  • Mott RL, Cordts JM, Larson AM (1984) Nitrogen and growth regulator effects on shoot and root growth of soybean in vitro. Plant Tissue Cult Meet, Knoxville, Tenn

    Google Scholar 

  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473–497

    Article  CAS  Google Scholar 

  • Newell CA, Luu HT (1985) Protoplast culture and plant regeneration in Glycine canescens FJ Herm. Plant Cell Tissue Org Cult 4: 145–149

    Article  Google Scholar 

  • Ohira K, Yamaya T, Ojima K (1975) Studies on the greening of cultured soybean and ruta cells. 1. Pigmentation as influenced by the composition of the medium. Tohoku J Agric Res 25: 136–178

    Google Scholar 

  • Ojima K, Ohira K (1977) Solubilization and utilization of Fe in the medium by suspension cultured cells of rice and soybean. J Sci Soil Manure 48: 96–100

    CAS  Google Scholar 

  • Orf JH, Hymowitz T, Pull SP, Pueppke SG (1978) Inheritance of a soybean seed lectin. Crop Sci 18: 899–900

    Article  CAS  Google Scholar 

  • Oswald TH, Smith AE, Phillips DV (1977) Callus and plantlet regeneration from cell culture of ladino clover and soybean. Physiol Plant 39: 129–134

    Article  CAS  Google Scholar 

  • Palmer RG, Heer HE (1973) A root tip squash technique for soybean chromosomes. Crop Sci 13: 389–391

    Article  Google Scholar 

  • Phillips GC, Collins GB (1981) Induction and development of somatic embryos from suspension cultures of soybean. Plant Cell Tissue Organ Cult 1: 123–129

    Article  CAS  Google Scholar 

  • Ranch JP, Palmer RG (1987) A ploidy variant regenerated from embryogenic tissue cultures of soybean. Soybean Genet Newl 14: 116–163

    Google Scholar 

  • Ranch JP, Oglesby L, Zielinski AC (1985) Plant regeneration from embryo-derived tissue cultures of soybeans. In Vitro 21: 653–658

    Google Scholar 

  • Ranch JP, Oglesby L, Zielinski AC (1986) Plant regeneration from tissue cultures of soybean by somatic embryogenesis. In: Vasil I (ed) Plant regeneration and genetic variability. Cell Culture and Somatic Cell Genetics of Plants, vol 3. Academic Press, Orlando, pp 97–110

    Google Scholar 

  • Rubluo A, Kartha KK, Mroginski LA, Dyck J (1984) Plant regeneration from pea leaflets cultured in vitro and genetic stability of régénérants. J Plant Physiol 117: 119–130

    CAS  Google Scholar 

  • Saka H, Voqui-Dinh TH, Cheng TY (1980) Stimulation of multiple shoot formation on soybean stem nodes in culture. Plant Sci Lett 19: 193–201

    Article  CAS  Google Scholar 

  • Schwenk FW (1981) Callus formation from mechanically isolated mesophyll cells of soybean and sweet potato. Plant Sci Lett 23: 147–151

    Article  Google Scholar 

  • Schwenk FW, Pearson CA, Roth MR (1981) Soybean mesophyll protoplasts. Plant Sci Lett 23: 153–155

    Article  CAS  Google Scholar 

  • Smith KJ, Huyser W (1987) World distribution and significance of soybean. In: Wilcox JR (ed) Soybeans: improvement, production, and uses, 2nd edn. American Soc Agron, Madison, WI, USA, pp 1–21

    Google Scholar 

  • Takahashi R, Tsuchiya T, Moriya I (1964) Heritable mixaploidy in barley. III. On a dwarf mutant from a cultivar, Taisho-mugi. Nogaku Kenkyu 50: 123–129

    Google Scholar 

  • Tricoli DM, Hein MB, Carnes MG (1986) Culture of soybean mesophyll protoplasts in alginate beads. Plant Cell Rep 5: 334–337

    Article  Google Scholar 

  • Vodkin LO, Rhodes PR, Goldberg RB (1983) A lectin gene insertion has the structural features of a transposable element. Cell 34: 1023–1031

    Article  PubMed  CAS  Google Scholar 

  • Widholm JM (1983) Isolation and characterization of mutant plant cell cultures. In: Sen SK, Giles KL (eds) Plant cell culture in crop improvement. Plenum Press, New York, pp 71–86

    Google Scholar 

  • Widholm JM, Rick S (1983) Shoot regeneration from Glycine canescens tissue cultures. Plant Cell Rep 2: 19–20

    Google Scholar 

  • Wright MS, Koehler SM, Hinchee MA, Carnes MG (1986) Plant regeneration by organogenesis in Glycine max. Plant Cell Rep 5: 150–154

    Article  CAS  Google Scholar 

  • Wright MS, Ward DV, Hinchee MA, Carnes MG, Kaufman RJ (1987) Regeneration of soybean (Glycine max L. Merr.) from cultured primary leaf tissue. Plant Cell Rep 6: 83–89

    Google Scholar 

  • Xu ZH, Davey MR, Cocking EC (1982) Callus formation from root protoplasts of Glycine max (soybean). Plant Sci Lett 24: 111–115

    Article  CAS  Google Scholar 

  • Yuyu J, Dupu L, Ximing L, Guilan Z (1984) Studies on induction of pollen plants in soybean (Glycine max [L.] Merr.). Int Symp Genetic Manipulation in Crops, Oct 22–26, Beijing China, pp 22–26

    Google Scholar 

  • Zieg RG, Outka DE (1980) The isolation, culture and callus formation of soybean pod protoplasts. Plant Sci Lett 18: 105–114

    Article  Google Scholar 

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Barwale, U.B., Widholm, J.M. (1990). Soybean: Plant Regeneration and Somaclonal Variation. In: Bajaj, Y.P.S. (eds) Legumes and Oilseed Crops I. Biotechnology in Agriculture and Forestry, vol 10. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-74448-8_6

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  • DOI: https://doi.org/10.1007/978-3-642-74448-8_6

  • Publisher Name: Springer, Berlin, Heidelberg

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