Cryopreservation of Germplasm of Legumes and Oilseed Crops

  • Y. P. S. Bajaj
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 10)


The seeds of legumes and most of the oilseed crops are “orthodox” and present no problem for long-term storage; however (1) “recalcitrant” species, in which seed is short-lived due to degeneration of the embryo, and where the germplasm cannot be conserved, require cryopreservation, (2) for the long-term preservation of elite and useful variants obtained by in vitro cell culture, innovative methods need to be evolved. Cryostorage of in vitro cultures has been recommended as one of the unconventional approaches for the long-term conservation of genetic stocks and for international exchange of germplasm (Bajaj 1976a, 1979a,b, 1983a, 1984a,b, 1985, 1986b). In this chapter, literature on the cryopreservation of tissue cultures of legumes and oilseed crops is reviewed, and prospects for this technology in the long-term conservation of germplasm are discussed.


Somatic Embryo Immature Embryo Oilseed Crop Somatic Hybrid Cell Cicer Arietinum 
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  1. Bajaj YPS (1976 a) Gene preservation through freeze storage of plant cell, tissue and organ cultures. Acta Hortic 63:75–84Google Scholar
  2. Bajaj YPS (1976b) Regeneration of plants from cell suspensions frozen at −20°, −70° and −196°C.Google Scholar
  3. Physiol Plant 37:263-268 Bajaj YPS (1979 a) Technology and prospects of cryopreservation of germplasm. Euphytica 28:267–285Google Scholar
  4. Bajaj YPS ( 1979 b) Establishment of germplasm banks through freeze-storage of plant tissue culture and their implications in agriculture. In: Sharp WR, Larsen PO, Paddock EF, Raghavan V (eds) Plant cell and tissue culture - principles and applications. Ohio State Univ Press, Columbus, pp 745–774Google Scholar
  5. Bajaj YPS (1979 c) Freeze preservation of meristems of Arachis hypogaea and Cicer arietinum. Indian J Exp Biol 17:1405–1407Google Scholar
  6. Bajaj YPS (1982a) Survival of anther-, and ovule-derived cotton callus frozen in liquid nitrogen. Curr Sci 51: 139–140Google Scholar
  7. Bajaj YPS (1982b) Cryopreservation of germplasm of potato, cassava, peanut and chickpea. In: Fujiwara A (ed) Plant Tissue Culture 1982. Maruzen, Tokyo, pp 799–800Google Scholar
  8. Bajaj YPS ( 1983 a) Cryopreservation and international exchange of germplasm. In: Sen SK, Giles KL (eds) Plant Cell Culture in Crop Improvement. Plenum Press, New York, pp 19–41Google Scholar
  9. Bajaj YPS (1983 b) Production of normal seeds from plants regenerated from meristems of Arachis hypogaea and Cicer arietinum cryopreserved for 20 months. Euphytica 32:425–430Google Scholar
  10. Bajaj YPS (1983 c) Regeneration of plants from pollen-embryos of Arachis, Brassica and Triticum spp. cryopreserved for one year. Curr Sci 52: 484–485Google Scholar
  11. Bajaj YPS (1983 d). Survival of somatic hybrid protoplasts of wheat x pea, and rice x pea subjected to −196°C. Indian J Exp Biol 21: 120–122Google Scholar
  12. Bajaj YPS (1984a) Cryopreservation of germplasm of crops - Present status and prospects. In: Novak FJ, Havel L, Dolezel J (eds). Int Symp Plant tissue and cell culture - application to crop improvement; Czech Acad Sci, Prague, pp 607–616Google Scholar
  13. Bajaj YPS (1984b) Biotechnology of the conservation of germplasm and its implications in agriculture and forestry. Proc Int Symp Genetic manipulations of crops. Acad Sin, BeijingGoogle Scholar
  14. Bajaj YPS (1984c) Induction of growth in frozen embryos of coconut and ovules of citrus. Curr Sci 53: 1215–1216Google Scholar
  15. Bajaj YPS (1985) Cryopreservation of embryos. In: Kartha KK (ed) Cryopreservation of plant cells and organs. CRC Boca Raton, pp 227–242Google Scholar
  16. Bajaj YPS ( 1986 a) Biotechnology in agriculture and forestry. Vols I, 2, Springer, Berlin Heidelberg New York TokyoGoogle Scholar
  17. Bajaj YPS (1986b) In vitro preservation of genetic resouces - techniques and problems. Proc Int Symp Nuclear techniques and in-vitro culture for plant improvement, IAEA/FAO, Vienna, pp 43–57Google Scholar
  18. Bajaj YPS (1988) Regeneration of plants from frozen (-196°C) protoplasts of Atropa belladonna L., Datura innoxia Mill and Nicotiana tabacum L. Indian J Exp Biol 26: 289–292Google Scholar
  19. Bajaj YPS (1989) Cryopreservation of plant protoplasts. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry 8. Plant protoplasts and genetic engineering I. Springer, Berlin Heidelberg New York Tokyo, pp 97–106Google Scholar
  20. Bajaj YPS, Gosal SS (1982) Induction of genetic variability in grain-legumes through tissue culture. In: Rao AN (ed) Proc Int. Symp Tissue culture of economically important crops. COSTED, Singapore, pp 25–41Google Scholar
  21. Bajaj YPS, Labana KS, Dhanju MS (1980) Induction of pollen-embryos and pollen-callus in anther cultures of Arachis hypogaea and A. glabrata. Protoplasma 103: 397–399CrossRefGoogle Scholar
  22. Bajaj YPS, Ram AK, Labana KS, Singh H (1981) Regeneration of genetically variable plants from the anther-derived callus of Arachis hypogaea and A. villosa. Plant Sci Lett 23: 35–39CrossRefGoogle Scholar
  23. Engelmann F, Duval Y (1986) Cryoconservation d’embryons somatiques de palmier à huile ( Elaeis guineensis Jacq. ): Résultats et perspectives d’application. Oleagineux 41: 169–172Google Scholar
  24. Finkle BJ, Ulrich JM, Rains DW, Tisserat BB, Schaeffer GW (1979) Survival of alfalfa, rice and date-palm callus after liquid nitrogen freezing ( Abstr ). Cryobiology 16: 583Google Scholar
  25. Gosal SS, Bajaj YPS (1983) Interspecific hybridization between Vigna mungo and Vigna radiate through embryo culture. Euphytica 32: 129–137CrossRefGoogle Scholar
  26. Haskins RH, Kartha KK (1980) Freeze-preservation of pea meristems: cell survival. Can J Bot 58: 833–840CrossRefGoogle Scholar
  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol Plant 15: 473–497CrossRefGoogle Scholar
  28. Steponkus PL, Dowgert MF, Ferguson JH, Levin RL (1983) Cryomicroscopy of isolated plant protoplasts. Cryobiology 21: 209CrossRefGoogle Scholar
  29. Sugawara Y, Takeuchi M (1986) A rapid method for the determination of cell survival in shoot apex after freezing and thawing. Int Congr Plant tissue cell cult, abstr. Univ Minnesota, Minneapolis, p 233Google Scholar
  30. Takeuchi M, Matsushima H, Sugawara Y (1982) Totipotency of protoplasts after long-term freeze preservation. In: Fujiwara A (ed) Plant tissue culture 1982. Maruzen, Tokyo, pp 797–798Google Scholar
  31. Weber G, Roth JE, Schweiger HG (1983) Storage of cell suspensions and protoplasts of Glycine max (L.) Merr., Brassica napus (L.). Datura innoxia (Mill.), and Daucus carota ( L.) by freezing. Z Pflanzenphysiol 109: 29–39Google Scholar
  32. Withers LA (1983) Germplasm storage in plant biotechnology. In: Mantell SH, Smith H (ed) Plant biotechnology. Cambridge Univ Press, Cambridge, pp 187–218Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Y. P. S. Bajaj
    • 1
  1. 1.Punjab Agricultural UniversityLudhianaIndia

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