Expression of Epidermal Growth Factor Receptors on Renal Cell Carcinomas

  • W. Heckl
  • K. Becker
  • U. Wacker
  • B. Halliger
Conference paper


The epidermal growth factor (EGF), a low molecular weight polypeptide, originally isolated from mouse submandibular glands and from human urine stimulates the proliferation of a variety of cells in cultures of both epithelial and mesenchymal origin (Cohen and Carpenter 1975; Gregory 1975). The EGF binds as well as the transforming growth factor-α (TGF-α) to the EGF-receptor located on the cell membranes of normal and transformed cells. This EGF-receptor is a 170000-dalton protein with an extracellular and an intracellular domain. The intracellular region of the EGF-receptor possesses tyrosine kinase activity, and there is autophosphorylation of tyrosine residues located near the carboxy terminus (Carpenter 1983; Downward et al. 1984b).


Acetone Tyrosine Polypeptide Dehydration Hematoxylin 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Carpenter G (1983) The biochemistry and physiology of the receptor-kinase for epidermal growth factor. Mol Cell Endocrinol 31: 1–19PubMedCrossRefGoogle Scholar
  2. Cohen S, Carpenter G (1975) Human epidermal growth factor: isolation and chemical and biological properties. Proc Natl Acad Sci USA 72: 1317–1321PubMedCrossRefGoogle Scholar
  3. Downward J, Yarden Y, Mayes E, Scarce G, Totty N, Stockwell P, Ullrich A, Schlessinger J, Water- field MD (1984a) Close similarity of epidermal growth factor receptor and v-erb B oncogene protein sequences. Nature 307: 521–527PubMedCrossRefGoogle Scholar
  4. Downward J, Parker P, Waterfield MD (1984b) Autophosphorylation sites on the epidermal growth factor receptor. Nature 311: 483–485PubMedCrossRefGoogle Scholar
  5. Gregory H (1975) Isolation and structure of urogastrone and its relation to epidermal growth factor. Nature 257: 325–327PubMedCrossRefGoogle Scholar
  6. Neal DE, Bennett MK, Hall RR, Marsh C, Abel PD, Sainsbury JRC, Harris AL (1985) Epidermal- growth-factor receptors in human bladder cancer: comparison of invasive and superficial tumors. Lancet i: 366–368Google Scholar
  7. Pruss RM, Herschman HR (1977) Variants of BT-3 cells lacking mitogenic response to epidermal growth factor. Proc Natl Acad Sci USA 74: 3918–3921PubMedCrossRefGoogle Scholar
  8. Roberts AB, Anzano MA, Wakefield LM, Roche NS, Stern DF, Sporn MB (1985) Type ß transforming growth factor: a bifunctional regulator of cellular growth. Proc Natl Acad Sci USA 82: 119–123PubMedCrossRefGoogle Scholar
  9. Sainsbury JRC, Fardon JR, Sherbet GV, Harris AL (1985) Epidermal-growth-factor receptors and oestrogen receptors in human breast cancer. Lancet i: 364–366Google Scholar
  10. Schreiber AB, Libermann TA, Lax I, Yarden Y, Schiessinger J (1983) Biological role of epidermal growth factor-receptor clustering. Investigation with monoclonal antireceptor antibodies. J Biol Chem 258: 846–853PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • W. Heckl
  • K. Becker
  • U. Wacker
  • B. Halliger
    • 1
  1. 1.Department of UrologyUniversity of Würzburg Medical SchoolWürzburgGermany

Personalised recommendations