Biological Effects of Recombinant Human Tumor Necrosis Factor on Human Bladder Cancer Cells
The tumor necrosis factor (TNF) was detected by Carswell et al. (1975) in the serum of animals injected sequentially with bacillus Calmette-Guerin or Corynebacterium parvum and endotoxin. This treatment induces proliferation of macrophages, which are stimulated to secrete TNF by endotoxin (Beutler et al. 1986). TNF causes hemorrhagic necrosis of certain transplantable mouse and human tumors with no apparent effect on the host (Haranata et al. 1984). Partially purified preparations of human TNF as well as recombinant TNF have been shown to exhibit selective cytotoxic effects in vitro against several tumor cell lines with no cytotoxic effect on normal fibroblasts (Wang et al. 1985; Sugarman et al. 1985) but growth-enhancing effects on normal diploid skin (Detroit 551), lung (WI38), and foreskin (FS-4) fibroblastic cultures (Viket et al. 1986). Although the mechanism of selective toxicity of TNF to tumor cells is unknown, the selectivity is supposed to be at the TNF-receptor level.
KeywordsToxicity Bacillus Polypeptide Adriamycin Actinomycin
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